Stress responses of honey bees to organic acid and essential oil treatments against varroa mites

Güneş, Nazmiye; Aydın, Levent; Belenli, Deniz; Hranıtz, John M.; Mengilig, Sami; Selova, Semih

doi:10.1080/00218839.2017.1291229

Cited by 28 publications

(17 citation statements)

References 30 publications

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“…The expression of HSP70 under stress conditions has already been described in insects [ 54 ]. For instance, FA treatment in honey bees resulted in increased HSP70 levels after treatment with high (85%) and low (30%) concentrations compared to the untreated control [ 55 ]. Additionally, hypoxia leads to the expression of several HSP genes in Sarcophaga crassipalpis and Drosophila melanogaster , including HSP70 [ 56 , 57 ] and also a direct influence of FA on HSP expression in Saccharomyces cerevisiae has already been demonstrated (Lee, Park et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor

et al. 2021

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The ectoparasite Varroa destructor Anderson and Trueman is the most important parasites of the western honey bee, Apis mellifera L. The most widely currently used treatment uses formic acid (FA), but the understanding of its effects on V. destructor is limited. In order to understand the mechanism of action of FA, its effect on Varroa mites was investigated using proteomic analysis by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). V. destructor was collected from honey bee colonies with natural mite infestation before and 24 h after the initiation of FA treatment and subjected to proteome analysis. A total of 2637 proteins were identified. Quantitative analysis of differentially expressed candidate proteins (fold change ≥ 1.5; p ≤ 0.05) revealed 205 differentially expressed proteins: 91 were induced and 114 repressed in the FA-treated group compared to the untreated control group. Impaired protein synthesis accompanied by increased protein and amino acid degradation suggest an imbalance in proteostasis. Signs of oxidative stress included significant dysregulation of candidate proteins of mitochondrial cellular respiration, increased endocytosis, and induction of heat shock proteins. Furthermore, an increased concentration of several candidate proteins associated with detoxification was observed. These results suggest dysregulated cellular respiration triggered by FA treatment as well as an increase in cellular defense mechanisms, including induced heat shock proteins and detoxification enzymes.

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Section: Discussionmentioning

confidence: 99%

Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor

et al. 2021

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“…They are a diverse group of chemicals, characterized by their phenolic structures and include flavonoids and phenolic acids (nonflavonoids [ Table 1 , Table 2 ]) ( Cianciosi et al, 2018 ). These biological compounds are transmitted from nectar to honey and are important components of honey's health-promoting properties ( Gunes et al 2017 ). Phenolic compounds are found at high levels in honey and may contribute to its antibacterial activity.…”

Section: Parameters Related To the Antimicrobial Activity Of Honeymentioning

confidence: 99%

The antibacterial activities of honey

Almasaudi

2021

Saudi Journal of Biological Sciences

187

142

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Honey is a powerful antimicrobial agent with a wide range of effects. Various components contribute to the antibacterial efficacy of honey: the sugar content; polyphenol compounds; hydrogen peroxide; 1,2-dicarbonyl compounds; and bee defensin-1. All of these elements are present at different concentrations depending on the source of nectar, bee type, and storage. These components work synergistically, allowing honey to be potent against a variety of microorganisms including multidrug resistant bacteria and modulate their resistance to antimicrobial agents. The effectiveness and potency of honey against microorganisms depends on the type of honey produced, which is contingent on its botanical origin, the health of the bee, its origin, and processing method. The application of antibiotics with honey yielded better antimicrobial potential and synergistic effects were noted against biofilms. In medicine, honey has been used in the treatment of surface wounds, burns, and inflammation, and has a synergistic effect when applied with antibiotics. Tissue repair is enhanced by the low pH of honey (3.5–4): causing a reduction in protease activity on the wound site, elevating oxygen release from hemoglobin and stimulating fibroblast and macrophage activity. Furthermore, H 2 O 2 has antiseptic effects, and it disinfects the wound site and stimulates production of vascular endothelial growth factor. The use of honey will clean wounds or burn areas from free radicals and reduces scarring and contractures. The anti-inflammatory and antibacterial potential of honey will keep the injured area moist and as such prevents it from deterioration and fibrosis. Honey can promote fast healing and reduce scarring and is very convenient for plastic surgery. Skin maceration is protected by honey due to its high osmolarity and because it keeps the injury moist. In non-infected areas, honey still reduced pain and inflammation. In general, the use of honey in medical settings has reduced economic loss and provided proven economic benefits by lowering direct costs in comparison to conventional treatments and by using less antibiotics, faster healing and less hospitalization stay. This review is intended to provide an overview of the antibacterial activities of honey and its applications.

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“…However, at one of the Portuguese sites, three colonies experienced a sudden large weight loss (around 3 to 4 kg in a few days) following treatment with ApiGuard. It has been reported that thymol can be released too rapidly at high temperatures and result in toxicity effects on the brood and adults, strong agitation of the colony, and in some cases, bees may abandon their hive (absconding) (Giaomelli et al, 2016;Gunes et al, 2017). In Denmark, a member of the consortium experienced a loss of 9-11 colonies due to varroa in 2019, in spite of treatment with ApiGuard.…”

Section: Varroa Treatmentmentioning

confidence: 99%

Research project on field data collection for honey bee colony model evaluation

Capela

Kryger

et al. 2021

EFSA Supporting Publications

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As part of the MUST-B project, a research project on field data collection for honey bee colony model evaluation was carried out in 2018-2020. In a preparatory phase (2018), methods for monitoring of honey bee colonies were tested, field operators trained, and experimental colonies established. The main field experiment was conducted in 2019-2020, during which bee colonies in six experimental apiaries were closely monitored in both Denmark and Portugal. An experimental spraying (spraying of Pirimor G in 6 ha of flowering oilseed rape) was carried out at one of the sites in Denmark in 2019. During the two-year experiment, climate variables were recorded continuously, and availability of floral resources was mapped regularly in the landscapes surrounding each apiary (within an area of 1.5 km radius). Adult bee population, brood and provision were assessed approximately every three weeks in experimental colonies. Furthermore, the weight of colonies was logged continuously during the field seasons by automatic hive scales. At four sites, foraging activity was monitored continuously in 1-2 colonies in 2019 and 2020. Spatial foraging was decoded from honey bee waggle dances observed once per month in four apiaries, at the same time as floral mapping. Finally, samples for analysis of diseases (varroa, Nosema and viruses), pesticide residues and botanical composition of pollen were collected. All data were organized in a relational database. Whereas previous studies have monitored similar aspects of honey bee colony development and health, the current dataset is unique in encompassing a large number of variables measured simultaneously. In particular, the current study emphasized a detailed data collection on population dynamics and development for the testing and calibration of the ApisRAM model developed in the MUST-B project. Methods used encompassed manual and automatic monitoring. Recommendations for future data collection include an assessment of variables currently collected with confidence and variables in need of further development.

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Stress responses of honey bees to organic acid and essential oil treatments against varroa mites

Cited by 28 publications

References 30 publications

Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor

Influence of formic acid treatment on the proteome of the ectoparasite Varroa destructor

The antibacterial activities of honey

Research project on field data collection for honey bee colony model evaluation

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