Stress response in Rhodococcus strains

Pátek, Miroslav; Grulich, Michal; Nešvera, Jan

doi:10.1016/j.biotechadv.2021.107698

Cited by 63 publications

(35 citation statements)

References 160 publications

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“…A recent review [ 20 ] discussing various stress effects on Rhodococcus populations summarizes physiological and phenotypic reactions of bacterial cells in response to environmental stresses, in addition to the responses to the effects of toxic metals, antibiotics, and other organic compounds. Previously, separate studies also considered effects of stress reactions of rhodococci on their persistent potential [ 7 , 16 , 22 ].…”

Section: Adaptive Cell Modifications Of Rhodococci Exposed To Hydrocarbons and Other Environmental Pollutantsmentioning

confidence: 99%

“…The number of publications is growing on new chemical agents degradable by rhodococci ( Figure 1 ); and Rhodococcus genome projects have been launched [ 4 , 5 , 6 ]. The ability of rhodococci to decompose recalcitrant xenobiotics and resist their toxic effects, in addition to Rhodococcus survival strategies under the combined actions of ecotoxicants and other exogenous harmful factors, are well documented [ 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…They tolerate high (100-250 mM or more) concentrations of toxic metals and metalloids, and organic solvents (from 20 to 80 vol.%). The strains are active in a wide range of temperatures (from 4-15 to 40 • C and above), acidity (pH from 2.0 to 9.0), and humidity (from 15 to 50%), and are able to grow at high (2-6%) salt concentrations [14,20,26,[28][29][30]. These Rhodococcus strains are the core of the Regional Specialised Collection of Alkanotrophic Microorganisms (acronym IEGM, available online at: http://www.iegmcol.ru (accessed on 20 July 2021)).…”

Section: Introductionmentioning

confidence: 99%

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Responses to Ecopollutants and Pathogenization Risks of Saprotrophic Rhodococcus Species

et al. 2021

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Under conditions of increasing environmental pollution, true saprophytes are capable of changing their survival strategies and demonstrating certain pathogenicity factors. Actinobacteria of the genus Rhodococcus, typical soil and aquatic biotope inhabitants, are characterized by high ecological plasticity and a wide range of oxidized organic substrates, including hydrocarbons and their derivatives. Their cell adaptations, such as the ability of adhering and colonizing surfaces, a complex life cycle, formation of resting cells and capsule-like structures, diauxotrophy, and a rigid cell wall, developed against the negative effects of anthropogenic pollutants are discussed and the risks of possible pathogenization of free-living saprotrophic Rhodococcus species are proposed. Due to universal adaptation features, Rhodococcus species are among the candidates, if further anthropogenic pressure increases, to move into the group of potentially pathogenic organisms with “unprofessional” parasitism, and to join an expanding list of infectious agents as facultative or occasional parasites.

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Section: Adaptive Cell Modifications Of Rhodococci Exposed To Hydrocarbons and Other Environmental Pollutantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Responses to Ecopollutants and Pathogenization Risks of Saprotrophic Rhodococcus Species

et al. 2021

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“…Therefore, it is important to analyze and understand functions of the plant-associated members of this genus, such as RL1 isolated from Eruca sativa leaves. Additionally, as mentioned in the introduction, the genus Rhodococcus is well-known for stress tolerant strains ( Pátek et al, 2021 ). For these reasons, we wanted to elucidate genomic properties with a special focus on functional analyses of stress tolerance and interaction with plants to understand the possible functions that the plant-associated R. qingshengii RL1 could provide within the plant holobiont and also compare it to the closely related strains djl6 and BG43.…”

Section: Discussionmentioning

confidence: 99%

“…Members of the genus Rhodococcus are resistant to various stresses ( Dabrock et al, 1994 ; Weyens et al, 2013 ; Pátek et al, 2021 ) and are able to degrade and metabolize a large spectrum of toxic compounds ( Kamble et al, 2013 ; Lincoln et al, 2015 ; Pham et al, 2015 ; Ceniceros et al, 2017 ; Gupta et al, 2019 ; Gorbunova et al, 2020 ). These traits make the genus Rhodococcus interesting for bioremediation applications ( Leigh et al, 2006 ; Płociniczak et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-Based Characterization of Plant-Associated Rhodococcus qingshengii RL1 Reveals Stress Tolerance and Plant–Microbe Interaction Traits

et al. 2021

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Stress tolerant, plant-associated bacteria can play an important role in maintaining a functional plant microbiome and protecting plants against various (a)biotic stresses. Members of the stress tolerant genus Rhodococcus are frequently found in the plant microbiome. Rhodococcus qingshengii RL1 was isolated from Eruca sativa and the complete genome was sequenced, annotated and analyzed using different bioinformatic tools. A special focus was laid on functional analyses of stress tolerance and interactions with plants. The genome annotation of RL1 indicated that it contains a repertoire of genes which could enable it to survive under different abiotic stress conditions for e.g., elevated mercury concentrations, to interact with plants via root colonization, to produce phytohormones and siderophores, to fix nitrogen and to interact with bacterial signaling via a LuxR-solo and quorum quenching. Based on the identified genes, functional analyses were performed in vitro with RL1 under different growth conditions. The R. qingshengii type strain djl6 and a closely related Rhodococcus erythropolis BG43 were included in the experiments to find common and distinct traits between the strains. Genome based phylogenetic analysis of 15 available and complete R. erythropolis and R. qingshengii genome sequences revealed a separation of the R. erythropolis clade in two subgroups. First one harbors only R. erythropolis strains including the R. erythropolis type strain. The second group consisted of the R. qingshengii type strain and a mix of R. qingshengii and R. erythropolis strains indicating that some strains of the second group should be considered for taxonomic re-assignment. However, BG43 was clearly identified as R. erythropolis and RL1 clearly as R. qingshengii and the strains had most tested traits in common, indicating a close functional overlap of traits between the two species.

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Development of synthetic small regulatory RNA for Rhodococcus erythropolis

Wang,

Hou,

Yang

et al. 2024

Biotechnology Journal

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Rhodococci have been regarded as ideal chassis for biotransformation, biodegradation, and biosynthesis for their unique environmental persistence and robustness. However, most species of Rhodococcus are still difficult to metabolically engineer due to the lack of genetic tools and techniques. In this study, synthetic sRNA strategy was exploited for gene repression in R. erythropolis XP. The synthetic sRNA based on the RhlS scaffold from Pseudomonas aeruginosa functions better in repressing sfgfp expression than those based on E. coli MicC, SgrS, and P. aeruginosa PrrF1‐2 scaffold. The RhlS‐based sRNAs were applied to study the influence of sulfur metabolism on biodesulfurization (BDS) efficiency in R. erythropolis XP and successfully identified two genes involved in sulfur metabolism that affect the BDS efficiency significantly. The RhlS‐based synthetic sRNAs show promise in the metabolic engineering of Rhodococcus and promote the industrial applications of Rhodococcus in environmental remediation and biosynthesis.

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Stress response in Rhodococcus strains

Cited by 63 publications

References 160 publications

Responses to Ecopollutants and Pathogenization Risks of Saprotrophic Rhodococcus Species

Responses to Ecopollutants and Pathogenization Risks of Saprotrophic Rhodococcus Species

Genome-Based Characterization of Plant-Associated Rhodococcus qingshengii RL1 Reveals Stress Tolerance and Plant–Microbe Interaction Traits

Development of synthetic small regulatory RNA for Rhodococcus erythropolis

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