Stress response in Cu2+ and Cd2+ exposed oysters (Crassostrea gigas): An immunohistochemical approach

Moraga, Dario; Meistertzheim, Anne-Leïla; Tanguy-Royer, Séverine; Boutet, Isabelle; Tanguy, Arnaud; Donval, Anne

doi:10.1016/j.cca.2005.05.014

Cited by 20 publications

(15 citation statements)

References 26 publications

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“…Examples are genes encoding heat shock 70 kDa protein 1A (Hsp70/HspA1A) in HeLa cells [32], prion protein (PrP) in neurons [33] and metallothionein 2A (MT2A) in hepatoblastoma cells [34]. These genes can be induced by copper not only in human cells but also in species like yeast, fungi, invertebrates and several others [35]–[37]. To test if the cells used in our study react similarly to elevated copper levels in the culture medium, isolated RNA of WI-38 HDFs incubated with copper salts (CuSO 4 or CuCl 2 ) or with sodium salts (Na 2 SO 4 or NaCl) was analyzed by real time RT-PCR (Fig.…”

Section: Resultsmentioning

confidence: 99%

Age-Related Cellular Copper Dynamics in the Fungal Ageing Model Podospora anserina and in Ageing Human Fibroblasts

et al. 2009

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In previous investigations an impact of cellular copper homeostasis on ageing of the ascomycete Podospora anserina has been demonstrated. Here we provide new data indicating that mitochondria play a major role in this process. Determination of copper in the cytosolic fraction using total reflection X-ray fluorescence spectroscopy analysis and eGfp reporter gene studies indicate an age-related increase of cytosolic copper levels. We show that components of the mitochondrial matrix (i.e. eGFP targeted to mitochondria) become released from the organelle during ageing. Decreasing the accessibility of mitochondrial copper in P. anserina via targeting a copper metallothionein to the mitochondrial matrix was found to result in a switch from a copper-dependent cytochrome-c oxidase to a copper-independent alternative oxidase type of respiration and results in lifespan extension. In addition, we demonstrate that increased copper concentrations in the culture medium lead to the appearance of senescence biomarkers in human diploid fibroblasts (HDFs). Significantly, expression of copper-regulated genes is induced during in vitro ageing in medium devoid of excess copper suggesting that cytosolic copper levels also increase during senescence of HDFs. These data suggest that the identified molecular pathway of age-dependent copper dynamics may not be restricted to P. anserina but may be conserved from lower eukaryotes to humans.

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Section: Resultsmentioning

confidence: 99%

Age-Related Cellular Copper Dynamics in the Fungal Ageing Model Podospora anserina and in Ageing Human Fibroblasts

et al. 2009

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“…It has been shown that Cd exposure results in elevated expression of metallothioneins (MTs) and heat-shock proteins (HSPs) in oysters (Roesijadi et al, 1997;Piano et al, 2004;Moraga et al, 2005). Due to the short half-life of MTs and HSPs (Andersen et al, 1978;Mehra and Bremner, 1985;Pelham, 1990), high synthesis rates would be required to maintain elevated steady-state levels of these proteins in Cd-exposed oysters.…”

Section: Discussionmentioning

confidence: 99%

Effects of acclimation temperature and cadmium exposure on cellular energy budgets in the marine molluskCrassostrea virginica: linking cellular and mitochondrial responses

Cherkasov

Biswas

Ridings

et al. 2006

Journal of Experimental Biology

129

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SUMMARY In order to understand the role of metabolic regulation in environmental stress tolerance, a comprehensive analysis of demand-side effects (i.e. changes in energy demands for basal maintenance) and supply-side effects (i.e. metabolic capacity to provide ATP to cover the energy demand) of environmental stressors is required. We have studied the effects of temperature (12, 20 and 28°C) and exposure to a trace metal, cadmium (50 μg l–1), on the cellular energy budget of a model marine poikilotherm, Crassostrea virginica (eastern oysters), using oxygen demand for ATP turnover, protein synthesis, mitochondrial proton leak and non-mitochondrial respiration in isolated gill and hepatopancreas cells as demand-side endpoints and mitochondrial oxidation capacity, abundance and fractional volume as supply-side endpoints. Cadmium exposure and high acclimation temperatures resulted in a strong increase of oxygen demand in gill and hepatopancreas cells of oysters. Cd-induced increases in cellular energy demand were significant at 12 and 20°C but not at 28°C,possibly indicating a metabolic capacity limitation at the highest temperature. Elevated cellular demand in cells from Cd-exposed oysters was associated with a 2–6-fold increase in protein synthesis and, at cold acclimation temperatures, with a 1.5-fold elevated mitochondrial proton leak. Cellular aerobic capacity, as indicated by mitochondrial oxidation capacity,abundance and volume, did not increase in parallel to compensate for the elevated energy demand. Mitochondrial oxidation capacity was reduced in 28°C-acclimated oysters, and mitochondrial abundance decreased in Cd-exposed oysters, with a stronger decrease (by 20–24%) in warm-acclimated oysters compared with cold-acclimated ones (by 8–13%). These data provide a mechanistic basis for synergism between temperature and cadmium stress on metabolism of marine poikilotherms. Exposure to combined temperature and cadmium stress may result in a strong energy deficiency due to the elevated energy demand on one hand and a reduced mitochondrial capacity to cover this demand on the other hand, which may have important implications for surviving seasonally and/or globally elevated temperatures in polluted estuaries.

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“…The gills of bivalves are often studied as models to assess and evaluate the effects of exposure to various toxins and heavy metals, in relation to their antioxidant and immunological properties [68,69]. As a contaminant, NaAsO 2 offered an all round disposition of damage, destruction of gill tissue architecture and a potent inhibitor of vital enzyme and biomolecule functions.…”

Section: Discussionmentioning

confidence: 99%

Toxicity of sodium arsenite in the gill of an economically important mollusc of India

Chakraborty¹,

Ray

2010

Fish & Shellfish Immunology

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Stress response in Cu2+ and Cd2+ exposed oysters (Crassostrea gigas): An immunohistochemical approach

Cited by 20 publications

References 26 publications

Age-Related Cellular Copper Dynamics in the Fungal Ageing Model Podospora anserina and in Ageing Human Fibroblasts

Age-Related Cellular Copper Dynamics in the Fungal Ageing Model Podospora anserina and in Ageing Human Fibroblasts

Effects of acclimation temperature and cadmium exposure on cellular energy budgets in the marine molluskCrassostrea virginica: linking cellular and mitochondrial responses

Toxicity of sodium arsenite in the gill of an economically important mollusc of India

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