Stress resistance mechanisms in the indicator fungi from highly radioactive Chernobyl zone sites

Егорова, А. С.; Гесслер, Н. Н.; Ryasanova, L. P.; Kulakovskaya, T. V.; Belozerskaya, T. A.

doi:10.1134/s0026261715020034

Cited by 10 publications

(14 citation statements)

References 11 publications

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“…Прооксидантно-антиоксидантна система (ПАС) живого організму включає два взаємопротилежні процеси: генерацію активних форм кисню (АФК), що ініціюють вільнорадикальне перекисне окиснення (ВРПО) та сповільнення чи припинення процесу окиснення комплексом речовин антиоксидантного захисту (АОЗ) (Halliwell, 2006;Winquist, 2008;Egorova et al, 2015). Увага до зміни показників ПАС пояснюється їх вирішальним значенням у функціонуванні організму на клітинному та організменому рівнях.…”

Section: вступunclassified

Condition of the prooxidant-antioxidant system of some strains of Basidiomycetes

Fedotov¹

2017

Regul. Mech. Biosyst.

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The article deals with the calculation and comparison indications of the condition of the prooxidant-antioxidant system (PAS) of strains of Basidiomycetes under periodic surface cultivation on a glucose-peptone medium. The research material consisted of the mycelium and culture filtrate (CF) from 57 strains, 52 of them belonging to 7 species of the order Agaricales and 5 belonging to 5 species of the order Polyporales. The intensity of the processes of lipid peroxidation was determined by a modified spectrophotometric method for contents of active products to thiobarbituric acid. Total antioxidant activity (АОА) of the mycological material was evaluated by intensity of inhibition from accumulated products of lipid peroxide oxidation (LPO) in a model reaction of oxidation by Twin-80 oxygen of the air. From the data obtained, indicators of prooxidant activity (POA), indicators of reserve of substrate peroxidation (SPO) and the balance coefficient of the prooxidant-antioxidant system (CbPАS) were calculated. It was established that strains of Basidiomycetes are characterized by significant predominance of prooxidant activity characteristic of PAS in the culture filtrate in comparison with the mycelium indicator. The highest values of POA in the Culture Filtrate were observed on the 12-th day of cultivation for the strain Р-089 genus Pleurotus and strain Gl-2 genus Ganoderma, and for the mycelium on the 9-th day of cultivation for the strains Р-сіtr, Р-089, Р-er and Р-082 of the genus Pleurotus. There is a direct dependence between the indicators of POA in the CF and mycelium for each strain, this dependence and level of indication do not reflect their systematic placement. We distinguished a more significant prevalence of indicators of reserve of substrates peroxidation of mycelium for most strains, than for such indicators with CF The highest value of reserve SPO of mycelium was recorded for strains Р-447, Р-998, Р-039, Р-94, Р-2175, Р-сіtr, Р-er, D-140 of the genus Pleurotus and strains F-107, F-610 of the genus Flammulina. For the majority of the strains there was a clear prevalence of the values of the balance coefficients for PAS of mycelium over those of CF. Maximum values of the balance coefficient PAS of mycelium on the 9-th day were recorded for strains Т-10 (F. fomentarius) and D-140, Р-035, Р-01, Р-105 (P. ostreatus) and on the 12-th day for strains Sc-10 (S. commune), Р-kl, Р-14 (P. ostreatus), Р-er (P. eryngii) і 960 (A. cylindracea). The abovementioned strains are distinguished by the with high level of AOA in the mycelium on certain days of cultivation and can be recommended as producers of antioxidants of fungal origin.

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Section: вступunclassified

Condition of the prooxidant-antioxidant system of some strains of Basidiomycetes

Fedotov¹

2017

Regul. Mech. Biosyst.

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“…Постоянная экспозиция эпифитных микроорганизмов иони зирующим излучением влияет не только на состав микробных ценозов, но и приводит к изменениям экологофизиологических свойств бактерий, в том числе их чувствительности к другим стрессфакторам. Изменение свойств ми кроорганизмов может сопровождаться приобретением признаков, способных влиять на характер растительномикробного взаимодействия [5,11].…”

Section: чувствительность к ультрафиолетовому излучению грамотрицателunclassified

Sensitivity to Uv-C of Gramnegative Epiphytic Bacteria From Chernobyl Exclusion Zone

Zelena

Gladka

Шепелевич

et al. 2017

Microbiology&Biotechnology

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“…In addition to natural habitats that can be defined as "extreme" from anthropocentric criteria, many independent scientific works have demonstrated life to be present in artificial environments with adverse factors for beings, even high levels of IR. Thus, some extremophilic bacterial and archaeal species have been isolated from high-level radioactive waste sites at Savannah River in South Carolina and at Hanford in Washington [7,8], spent nuclear fuel storage pools at Sellafield, UK [9] and Madras Atomic Power Station, Kalpakkam [10], German salt dome Gorleben [11], from the Chernobyl Nuclear Power Plant buildings [12,13,14]. According to the most widely accepted theory, the origin of cross-resistance to ionizing radiation and other types of stress may be related to overlap between mechanisms of resistance to IR and other stressors [15].…”

Section: Introductionmentioning

confidence: 99%

DetR DB: A Database of Ionizing Radiation Resistance Determinants

Ryabova¹,

Козлова²,

Kadirov³

et al. 2020

Preprint

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Nuclear pollution is an urgent environmental issue as a consequence of rapid industrialization and nuclear accidents in the past. Remediation of nuclear polluted sites using microbial vital activity (bioremediation) is a promising approach to recover contaminated areas in an environmentally friendly and cost-saving way. At the same time, the number of known bacterial and archaeal species able to withstand extremely high doses of ionizing radiation is steadily growing every year, together with growing knowledge about mechanisms of radioresistance. This opens up new opportunities for developing new biotechnological solutions. However, these data are often not systemized and it can be difficult to access. Here, we present the Determinants of Radioresistance Database, or DetR DB (http://extremebiolab.com/detr-db/), gathering a comprehensive catalog of radioresistant microbes and their molecular and genetic determinants of enhanced ionizing radiation tolerance. The database provides search tools including taxonomy, common gene name, and BLAST. DetR DB will be a useful tool for research community by facilitating the extraction of the necessary information to help further analysis of radiation-resistant mechanisms.

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Stress resistance mechanisms in the indicator fungi from highly radioactive Chernobyl zone sites

Cited by 10 publications

References 11 publications

Condition of the prooxidant-antioxidant system of some strains of Basidiomycetes

Condition of the prooxidant-antioxidant system of some strains of Basidiomycetes

Sensitivity to Uv-C of Gramnegative Epiphytic Bacteria From Chernobyl Exclusion Zone

DetR DB: A Database of Ionizing Radiation Resistance Determinants

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