1998
DOI: 10.1016/s0168-9452(98)00169-1
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Stress proteins co-expressed in suberized and lignified cells and in apical meristems

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Cited by 49 publications
(34 citation statements)
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“…The abundance of P450 monooxygenases and of oxidases reflects the high complexity of synthesizing the cork polymeric matrix and indicates that cork cell metabolism must generate reactive oxygen species in high amounts. This corresponds to previous observations showing that cork cells suffer from fairly high oxidative stress (Pla et al, 1998(Pla et al, , 2000. Here, we found that ascorbate peroxidase, a crucial enzyme for H 2 O 2 detoxifying in plant cells (Davletova et al, 2005), was strongly up-regulated in cork.…”
Section: Linkages Between Aliphatic and Aromatic Unitssupporting
confidence: 92%
“…The abundance of P450 monooxygenases and of oxidases reflects the high complexity of synthesizing the cork polymeric matrix and indicates that cork cell metabolism must generate reactive oxygen species in high amounts. This corresponds to previous observations showing that cork cells suffer from fairly high oxidative stress (Pla et al, 1998(Pla et al, , 2000. Here, we found that ascorbate peroxidase, a crucial enzyme for H 2 O 2 detoxifying in plant cells (Davletova et al, 2005), was strongly up-regulated in cork.…”
Section: Linkages Between Aliphatic and Aromatic Unitssupporting
confidence: 92%
“…The QsHsp10.4-CI coding region was excised from the phage clone kgt11-QsHsp10.4-CI by EcoRI digestion, blunt-ending and BsmFI digestion. Then it was cloned in the vector resulting from BsmI digestion, blunt-ending and BsmFI digestion of pBluescriptHsp17.4 (Pla et al 1998). This intermediate plasmid was digested with EcoRI and XhoI and the insert was subsequently cloned into the pET29a expression vector, previously digested with the same restriction enzymes.…”
Section: Cloning Of Hsp-cismentioning
confidence: 99%
“…130 lg of protein was loaded in each gel. In both oneand two-dimensional (2D) electrophoresis, proteins were transferred onto polyvinylidenedifluoride (PVDF) membranes (Millipore) and incubated with polyclonal antibody raised against recombinant QsHsp17.4-CI protein in rabbit (Pla et al 1998). Bound antibodies were detected using peroxidase-conjugated goat anti-rabbit IgG (GAR-Po; Nordic Immunology) and a chemiluminescence system (BM Chemiluminescence Western Blotting Substrate; Boehringer Mannheim).…”
Section: Electrophoresis and Immunological Detection Of Proteinsmentioning
confidence: 99%
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