Stress of algicidal substances from a bacterium Exiguobacterium sp. h10 on Microcystis aeruginosa

Abstract

In this study, the effects of algicidal substances from an algicidal bacterium Exiguobacterium sp. h10 on the toxic cyanobacterium, Microcystis aeruginosa 7820, were first investigated. The algicidal mode of action was confirmed as an indirect attack through the production of algicidal substances. The characteristics of the algicidal substance were determined, especially the functional groups analysis that confirmed the algicidal substances were glycolipid mixtures. With the stress of algicidal substances, the algal chlorophyll a synthesis, cell structure and morphology were seriously damaged. This study proved that algicidal bacteria are promising sources of potential cyanobacterial bloom-control, and provided good procedures for the identification and analysis of an algicidal bacterium and substances.

Algicidal characterization and mechanism of Bacillus licheniformis Sp34 against Microcystis aeruginosa in Dianchi Lake

“…Sp34 could kill M. aeruginosa with an initial Chl-a concentration as high as 2.0 ± 0.2 mg/L at a cell density no less than 1.35 × 10 5 CFU/ml. In most research, the initial Chl-a concentration was low (0.5-1.0 mg/L) [11,40], while the Chl-a content during the blooms in | 1119 natural water bodies was usually 0.04-0.39 mg/L [41][42][43], but can reach almost 2.0 mg/L when the blooms become serious [44]. Therefore, the initial Chl-a concentration used in this study is closer to naturally harmful bloom situations, and application of Sp34 in severe bloom control is promising.…”

Bactericidal Effects of Exiguobacterium sp GM010 Pigment Against Food-Borne Pathogens

“…These results revealed, the isolated bacterium is belongs to the genus Exiguobacterium. Even though there are reports on Exiguobacterium species showing the algicidal (Li et al, 2016), antifungal (Selvakumar et al, 2009) and antibacterial (Shanthakumar et al, 2015) effect, there are no report on the antibacterial action of Exiguobacterium sp GM010 pigment and the mechanism of antibacterial action.…”

“…The characteristic functional groups of the algicidal substance produced by the Exiguobacterium sp. h10 mainly included carbonyl, amino, and hydroxyl groups (Li et al, 2016). Similarly 3,6,18-trione, 9,10-dihydro-12-hydroxyl-2methyl-5-(phenyl methyl) (5-alpha, 10-alpha)-dihydroergotamine and dipropyl-Spropyl ester molecules exhibit antibacterial action against clinical pathogens (Shanthakumar et al, 2015).…”

“…The Streptomyces sp was widely studied microbial species from Indian coastal waters as a source of antibiotics (Chandramohan, 1997;Valliappan et al, 2014). There are report on the algicidal (Li et al, 2016), antifungal (Selvakumar et al, 2009) and antibacterial (Shanthakumar et al, 2015) effect of Exiguobacterium species, however mechanism of antibacterial effect of Exiguobacterium pigment on food-borne pathogens was not reported. In this study pigment producing Exiguobacterium sp GM010 was isolated, and characterized.…”

Adsorption Strategy for Removal of Harmful Cyanobacterial Species Microcystis aeruginosa Using Chitosan Fiber

“…Various treatments have been utilized, including several physical, chemical and biological methods. The following methods/reagents have been used to address the problems caused by Cyano-HABs: ultra-sonication [10], waterwheel system for water aeration [11,12], loess spraying [12], algicidal materials [13], coagulants [14] and algicidal bacteria [15]. Spraying loess into water bodies has been primarily employed to control Cyano-HABs in South Korea [12].…”