Stress Metabolism. VI.* Temperature Stress and the Accumulation of Proline in Barley and Radish

Chu, T. M.; Aspinall, D.; Paleg, L. G.

doi:10.1071/pp9740087

Cited by 88 publications

(51 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The measurements of growth related traits in 40 days old barley plants confirmed earlier reports by Chu et al (1974) that plant height was inhibited and leaf chlorophyll decreased by high temperature in barley; these effects were accentuated by accompanying water-stress in the leaves. These effects were confirmed for a range of temperatures extending to a maximum of 41°C.…”

Section: Discussionsupporting

confidence: 76%

Physiological and Biochemical Responses to Heat Stress on Barley Seedlings and Their Impact on Growth and Yield

2016

Egypt. J. Bot.

View full text Add to dashboard Cite

HE PRESENT study was carried out to investigate the effect of two … temperature degrees (30&40°C) on physiological and biochemical aspects of barley seedlings and their impact on growth and yield. Plant height, number of tillers, fresh weight, dry weight, flag leaf area were generally reduced following exposure to heat stress ranging between 2 and 8 hours., while relative water content was increased indicating attempts by plant to resist temperature elevation. The contents of chlorophyll a and chlorophyll b decreased significantly in leaves exposed to all treatments of heat stress while carotenoids decreased as the stress duration was prolonged. Correlation was evident between growth, some yield parameters (peduncle length, biological yield, spike length, number of spike per plant, kernels weight) and yield index. Changes in SDS protein profiles indicated the occurrence of a number of biochemical responses; some of them were positively associated with heat stress, while the others were negatively associated with heat stress.

show abstract

Section: Discussionsupporting

confidence: 76%

Physiological and Biochemical Responses to Heat Stress on Barley Seedlings and Their Impact on Growth and Yield

2016

Egypt. J. Bot.

View full text Add to dashboard Cite

show abstract

“…The sensitivity of sperm to osmotic stress during cryopreservation and thawing and thawing differ between individual bulls can classified bulls as good freezers or bad freezers implies the presence of certain membrane structure characteristics and that differences are genetically coded [4]. Crossbred of Holstein Friesian X Hariana diluted in Tris exposed to post-thawing hypo-osmotic swollen test results varied significantly between bulls [34].…”

Section: Discussionmentioning

confidence: 99%

Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

Mohammed¹,

Ahmed²

2017

Andrology

View full text Add to dashboard Cite

Objectives: As very few studies were done on the freezability of pure Egyptian cattle bull sperm. So, we designed this study to evaluate the individual variations in freezability of native bull semen extended in Tris based diluent and sodium citrate-based diluent. Methods:Semen was collected by artificial vagina, examined at once in farm laboratory. Only semen samples fit the minimum parameters are extended in two extenders first the universal one (TRIS) and second modified Sodium citrate extender by adding glycerol (CU-16) which created at Cornell University to evaluate the individual bull variation and interaction between extender and bull.Results: Post-Thawing individual sperm motility, live, abnormal, acrosome integrity percentage was evaluated in addition to Hypo-Osmotic Swelling test (HOS). The highest value for motility, live abnormal and acrosome percentage were 44.00 ± 1.12, 52.25 ± 1.60, 21.25 ± 0.81 and 62.80 ± 2.58 from bull 2, 1, 3 and 2, respectively for semen extended in TRIS, and 42.25 ± 1.61, 52.00 ± 1.76, 22.15 ± 0.85 and 57.40 ± 3.07, from bull 1, 1, 4, 3, respectively for semen extended in CU-16. The results of HOS were 59.25 ± 1.76 and 55.95 ± 2.13 from bull 1 extended in TRIS and CU-16, respectively. Conclusion:A significant variation (p<0.05) in tested parameter was clear when semen extended in TRIS extender with the absence of such variation when semen extended in CU-16 except in live sperm percentage. With ignoring the extender effect a clear significant variations were detected between bulls in all tested parameters.

show abstract

“…Dispensing the cell suspension as hanging droplets onto a Petri dish lid. 4. Dispensing the cell suspension into a 5-cm diameter Petri dish.…”

Section: Methodsmentioning

confidence: 99%

Proline: A Novel Cryoprotectant for the Freeze Preservation of Cultured Cells of Zea mays L

Withers¹,

King²

1979

Plant Physiol.

151

View full text Add to dashboard Cite

Proline is an effective cryoprotectant for the storage of cultured cells of Zea mays L. in liquid N2. Increased freeze tolerance can be achieved by pregrowth for 3 to 4 days in medium containing proline. Cells cryoprotected with proline have an increased recovery potential when compared with cells cryoprotected with dimethylsulfoxide and glycerol. They also show a reduced postthaw viability loss and greater tolerance of a range of postthaw culture conditions. It is suggested that the mechanism of action of proline may be similar to that in its putative role of conferring protection against natural stresses. It may be protecting the cell against solution effects caused by dehydration during freezing. These findings are discussed in relation to other freeze tolerance enhancing treatments.Freeze preservation in LN3 as a means of genome storage can now be applied to a very limited range of plant tissue cultures. The reasons for the present limitations are not fully understood (1,20,24).In biological systems, the two major sources of cryoinjury are ice damage and solution effects, the latter caused by the excessive concentration of intracellular solutes (9, 10). Protection against cryoinjury may be achieved by either freezing slowly to induce dehydration by extracellular freezing, thereby minimizing intracellular ice formation, or alternatively by freezing and thawing very rapidly to prevent dehydration while maintaining a small, innocuous, ice crystal size. The addition of cryoprotective compounds may reduce cryoinjury in either rapid or slow freezing. In the majority of freeze preservation protocols applied to plant cells, cryoprotectants (usually DMSO and/or glycerol) are used in combination with freezing at a slow rate (1, 24). The high water content of plant cells results in an enhanced susceptibility to cryoinjury. At the level of dehydration required to avoid solution effect stresses, it is still normally necessary to thaw very rapidly to minimize ice recrystallization damage (1,20,24).Recovery of cultures after thawing is dependent upon both a high postthaw viability and further survival through a recovery period preceding renewal of normal metabolic functions and cell division (15,20 In the present study, proline has been used as both a cryoprotectant and pregrowth additive (23) for the freeze preservation of cultured cells of maize. It will be demonstrated that this compound is highly effective in both modes of use and is superior in a number of respects to conventional cryoprotectants. MATERIALS AND METHODSA suspension culture of Zea mays L. (a cell line derived from cultivar B73 [inbred] and kindly supplied by Dr. Ingo Potrykus) was maintained in exponential growth under the conditions described by Potrykus et aL (13). Cells were harvested for freezing 3 or 4 days after subculturing. Additionally, a cell suspension was pregrown for 3 or 4 days in medium supplemented with 5 or 10%1o (w/v) L-proline. Cryoprotectants were prepared using analytical grades of L-proline, glycerol and DMSO, at double th...

show abstract

Stress Metabolism. VI.* Temperature Stress and the Accumulation of Proline in Barley and Radish

Cited by 88 publications

References 8 publications

Physiological and Biochemical Responses to Heat Stress on Barley Seedlings and Their Impact on Growth and Yield

Physiological and Biochemical Responses to Heat Stress on Barley Seedlings and Their Impact on Growth and Yield

Pure Egyptian Cattle Bulls Show both Individual Variation and Different Interaction with Extender in the Post-Thawing Sperm Parameters

Proline: A Novel Cryoprotectant for the Freeze Preservation of Cultured Cells of Zea mays L

Contact Info

Product

Resources

About