Stress Metabolism V. Abscisic Acid and Nitrogen Metabolism in Barley and Lolium Temulentum L.

Aspinall, D.; Singh, TN; Paleg, L. G.

doi:10.1071/bi9730319

Cited by 44 publications

(11 citation statements)

References 11 publications

(14 reference statements)

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“…We have observed a substantial enrichment (based on the intensity of Coomassie staining) of one polypeptide with an apparent molecular mass of 55 to 57 kD. Proteins that may contribute to occurrence of bands in this region are, for example, the 13-subunit of coupling factor 1 (13) have observed the appearance of one 31-kD polypeptide that correlates with modification of lightharvesting Chl a/b complex in maize leaves exposed to chilling temperatures. According to these authors, accumulation of this protein could be an important factor for the determination of chill-induced decrease in the PSII photochemistry and carbon assimilation quantum yield (2).…”

Section: Gel Electrophoresismentioning

confidence: 86%

Changes in the Polypeptide Patterns of Barley Seedlings Exposed to Jasmonic Acid and Salinity

Maslenkova¹,

Miteva²,

Попова³

1992

Plant Physiol.

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Soluble and thylakoid membrane proteins of jasmonic acid (JA)-treated and salt-stressed barley (Hordeum vulgare L.) seedlings were investigated using 15% sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis. High JA concentrations induced marked quantitative and qualitative changes in polypeptide profiles conceming mainly the proteins with approximately equal mobility, as in NaCI-stressed plants. The most obvious increase in thylakold polypeptide band intensity was at 55 to 57 kilodaltons (kD). The relative share of some polypeptides with apparent molecular masses above 66 kD and of polypeptides with lower molecular masses In the region of 20.5 to 15 kD was enhanced. At the same time, one new band at 31 to 31.5 kD was well expressed at 25 and 250 micromolar JA concentrations and became discemible in the 100 micromolar NaCI-treated plants. The Intensity of some polypeptides of soluble proteins (molecular masses of 60, 47, 37, 30, and 23.4 kD) increased with increasing JA concentration, whereas the intensities of other polypeptide bands (55, 21.4, and 15 kD) decreased. Enhanced levels of 60-, 47-, 34-, and 30-kD polypeptides and reduced levels of 55-and 15-kD polypeptides were present in NaCI-treated plants. The appearance of one new polypeptide, of 25.1 kD, was observed only in NaCI-treated plants. At 100 millimolar NaCI, an eighffold increase In proline content was observed while at 250 micromolar JA, the proline content was threefold over the control. It is hypothesized that exogenously applied jasmonates act as stress agents. As such, they provoke alterations in the proline content and they can modulate typical stress responses by induction of stress proteins.

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Section: Gel Electrophoresismentioning

confidence: 86%

Changes in the Polypeptide Patterns of Barley Seedlings Exposed to Jasmonic Acid and Salinity

Maslenkova¹,

Miteva²,

Попова³

1992

Plant Physiol.

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“…For instance, proline accumulates in cultured tomato cells subjected to water deficit induced by PEG (19) and in tobacco cells in response to NaCi stress (32, data not shown). Accumulation of proline in plants can be stimulated by NaCl, water deficit, or ABA treatment (1,19,28). Unique proteins, including a major 26 kD protein, accumulate in cells adapted to NaCl stress (15,30), and the synthesis of this major 26 kD protein appears to be stimulated by ABA.…”

Section: Resultsmentioning

confidence: 99%

“…ABA accumulation, which has been shown to be heritable in some plants (22), has been found to be correlated with heritable stress tolerance (27). ABA also has been shown to stimulate physiological responses believed to have stress-adaptive value (1,13,27). More direct evidence that ABA is involved in stress adaptation is provided by the findings that ABA can increase the tolerance of plant tissues to stresses caused by water deficit (25), chilling (6,10), freezing (11), and a herbicide (29 show that ABA stimulates the adaptation of tobacco cells to salt stress by accelerating their growth in the presence of NaCl and other salts.…”

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confidence: 99%

Abscisic Acid Accelerates Adaptation of Cultured Tobacco Cells to Salt

LaRosa¹,

Handa²,

Hasegawa³

et al. 1985

Plant Physiol.

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“…Treatment with ABA also causes proline to accumulate (2). The metabolic mechanism by which proline accumulates during drought stress has been studied in barley leaves which accumulate proline when subjected to any of these stresses and the results have been summarized (17).…”

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confidence: 99%

The Mechanism of Abscisic Acid-induced Proline Accumulation in Barley Leaves

Stewart¹

1980

Plant Physiol.

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Soluble proline accumulates in plant tissues under several types of stresses including drought (4,13,(16)(17)(18)(19), and references cited therein), salinity (8), and low temperature (7). Treatment with ABA also causes proline to accumulate (2). The metabolic mechanism by which proline accumulates during drought stress has been studied in barley leaves which accumulate proline when subjected to any of these stresses and the results have been summarized (17). In excised leaves, proline accumulates mainly because its synthesis from glutamic acid is stimulated (4). Inhibition of proline oxidation also contributes to the accumulation, as does impaired utilization for protein synthesis (18,19).ABA also accumulates in leaves under drought stress (20) and, in barley, ABA induces proline to accumulate in turgid leaves (2). The interaction between ABA treatment and PEG-induced water stress in barley has been studied and the results indicate that ABA-treated stressed plants accumulate more proline than those stressed without ABA (12). The time course for changes in ABA and proline levels in barley leaves during water stress and recovery from stress permit one to postulate that ABA could be the cause of proline accumulation under stress conditions (1). However, exogenous ABA does not cause proline to accumulate in turgid leaves of sunflower and tobacco, species which readily accumulate proline during drought stress (1).The experiments reported here were conducted to determine the metabolic basis for ABA-induced proline accumulation in barley leaves. The main effect of ABA on proline metabolism is stimulated synthesis from glutamate. MATERIALS AND METHODSBarley (Hordeum vulgare, cv. "Prior") seedlings were grown in soil in a growth chamber at 21 C and 2,500 ft-c. After emergence, plants were watered daily with modified Hoagland solution (9). Fully expanded second leaves were excised at the base of the leaf blade from 2-week-old plants which had been in the light for 24 h prior to excision. After excision, leaves were pretreated by placing the cut ends in a solution that was 50 mm sucrose and I mm glutamate. This pretreatment was found to be necessary for reproducible ABA-induced proline accumulation even though leaves were taken from plants which had been in the light for 24 h immediately preceding excision. Radioactive compounds,in 5 t1 of H20 were added through the cut end of the leaf. Details of the amount added and specific radioactivity are given in the figure legends. Leaves were incubated in vials with the cut end immersed in I ml of H20 (control) or I ml of 20 mg/l ABA. ABA was the (RS) cis-trans isomer obtained from Sigma Chemical Co. It was dissolved in H20 by continued stirring in darkness. Each measurement was an average of three replicate leaves. When "Clabeled precursors were fed, leaves were incubated for various times, killed by immersion in 95% v/v ethanol, and then thoroughly extracted with 80%o v/v ethanol. Extracts were dried, washed once with chloroform, and then taken up in water. After two-dimensiona...

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Stress Metabolism V. Abscisic Acid and Nitrogen Metabolism in Barley and Lolium Temulentum L.

Cited by 44 publications

References 11 publications

Changes in the Polypeptide Patterns of Barley Seedlings Exposed to Jasmonic Acid and Salinity

Changes in the Polypeptide Patterns of Barley Seedlings Exposed to Jasmonic Acid and Salinity

Abscisic Acid Accelerates Adaptation of Cultured Tobacco Cells to Salt

The Mechanism of Abscisic Acid-induced Proline Accumulation in Barley Leaves

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