Stress Management Affects Outcomes in the Pathophysiology of an Endometriosis Model

Appleyard, Caroline B.; Cruz, Myrella L.; Hernández, Siomara; Thompson, Kenira J.; Bayona, Manuel; Flores, Idhaliz

doi:10.1177/1933719114542022

Cited by 33 publications

(57 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Prior studies in our laboratory found that psychological stress can exacerbate endometriosis symptoms in this model (9). Stress is known to be involved in the etiology of a variety of disease states (39, 40), yet few studies have examined how physical activity can ameliorate the effects of stress.…”

Section: Discussionmentioning

confidence: 69%

“…Animals were handled (5 min/d) for 7 days prior to beginning the experiments in order to reduce manipulation stress, and vaginal cytological smears carried out daily to verify reproductive cycles (9). Experiments were carried out at the same time of day (9 am to 12 pm) to minimize the influence of circadian rhythms.…”

Section: Methodsmentioning

confidence: 99%

“…After performing a laparotomy, the peritoneal cavity was systematically examined for the presence of implants and the original sutures, and their longest length and width measured with a digital caliper. Classification of lesions was carried out as previously described (9, 27) whereby lesions were assigned the following grades: <2 mm in length = grade 2; ≥2 mm but <4.5 mm = grade 3; ≥4.5 mm but <6.0 mm = grade 4; ≥6.0 mm = grade 5. If a lesion did not develop, a grade of 1 was assigned.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Impact of Physical Activity on Pain Perception in an Animal Model of Endometriosis

Hernández

Cruz

Torres-Reverón

et al. 2015

Journal of Endometriosis and Pelvic Pain Disorders

Self Cite

View full text Add to dashboard Cite

Background Symptoms of endometriosis, such as pain and infertility, are considered significant sources of stress. In many chronic conditions, exercise can act as a stress buffer and influence pain perception. We tested the impact of swimming exercise on pain perception and pain receptors in an animal model of endometriosis. Methods Endometriosis (Endo) was induced in female rats by suturing uterine horn tissue next to the intestinal mesentery. Sham rats received sutures only. Rats were exposed to swimming exercise for 7 consecutive days, while no-exercise rats were left in the home cage. Fecal pellets were counted after swimming as an index of anxiety, and serum corticosterone levels measured. Pain perception was assessed using the hot plate test for hyperalgesia and Von Frey test for allodynia. Mu-opioid receptor (MOR) and neurokinin-1 receptor expression in the spinal cord was measured by immunofluorescence. Results Fecal pellet counts were higher in those animals that swam (p<0.05), but no significant difference in corticosterone was found. Although Endo-exercise rats had higher colonic damage (p<0.05) with more cellular infiltration, the lesions were smaller than in Endo–no exercise rats (p<0.05). Exercise did not ameliorate the hyperalgesia, whereas it improved allodynia in both groups. MOR expression was significantly higher in Endo-exercise vs. Endo–no exercise rats (p<0.01), similar to Sham–no exercise levels. Conclusions Our results point toward beneficial effects of swimming exercise during endometriosis progression. Physical interventions might be investigated further for their ability to reduce perceived stress and improve outcomes in endometriosis.

show abstract

Section: Discussionmentioning

confidence: 69%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Impact of Physical Activity on Pain Perception in an Animal Model of Endometriosis

Hernández

Cruz

Torres-Reverón

et al. 2015

Journal of Endometriosis and Pelvic Pain Disorders

Self Cite

View full text Add to dashboard Cite

show abstract

“…Animals were part of a larger set of rats from our previous publication. 28 Rats were randomly assigned to either sham surgery or surgical induction of endometriosis (endo). All animals were maintained in a bio-bubble facility room on a 12-hour light-dark cycle and at constant temperature (23 C).…”

Section: Animal Modelmentioning

confidence: 99%

Endometriosis Is Associated With a Shift in MU Opioid and NMDA Receptor Expression in the Brain Periaqueductal Gray

et al. 2016

Self Cite

View full text Add to dashboard Cite

Studies have examined how endometriosis interacts with the nervous system, but little attention has been paid to opioidergic systems, which are relevant to pain signaling. We used the autotransplantation rat model of endometriosis and allowed to progress for 60 days. The brain was collected and examined for changes in endogenous opioid peptides, mu opioid receptors (MORs), and the N-methyl-D-aspartate subunit receptor (NR1) in the periaqueductal gray (PAG), since both of these receptors can regulate PAG activity. No changes in endogenous opioid peptides in met-and leu-enkephalin or b-endorphin levels were observed within the PAG. However, MOR immunoreactivity was significantly decreased in the ventral PAG in the endometriosis group. Endometriosis reduced by 20% the number of neuronal profiles expressing MOR and reduced by 40% the NR1 profiles. Our results suggest that endometriosis is associated with subtle variations in opioidergic and glutamatergic activity within the PAG, which may have implications for pain processing.

show abstract

“…Endometriosis was surgically induced as previously described elsewhere (29,30). Briefly, rats were anesthetized with isoflurane and four pieces of the right uterine horn were auto transplanted to 4 different blood vessels in the intestinal mesentery.…”

Section: Methodsmentioning

confidence: 99%

Antagonizing the corticotropin releasing hormone receptor 1 with antalarmin reduces the progression of endometriosis

Torres-Reverón

Rivera-Lopez

Flores

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

Endometriosis is a disorder in which endometrial tissue is found outside the uterus causing pain, infertility and stress. Finding an effective and long-term treatment for endometriosis still remains one of the most significant challenges in the field. Corticotropin releasing hormone (CRH) is one of the main signaling peptides within the hypothalamic pituitary adrenal (HPA) axis released in response to stress. CRH can affect nervous and visceral tissues such as the uterus and gut via activation of two types of CRH receptors: CRHR1 and CRHR2. Our aim was to determine if blocking CRHR1 with antalarmin will reduce endometriosis progression. First, we induced endometriosis in female rats by suturing uterine horn tissue next to the intestinal mesentery and allowed to progress for 7 days. We determined that after 7 days, there was a significant increase in CRHR1 within endometriotic vesicles as compared to normal uterus. A second group of rats received endometriosis but also antalarmin (20 mg/kg, i.p.) during the first 7 days after surgery. As separate group of sham surgery rats served as controls. Endometriosis was allowed to progress until 60 days after surgery. At time of sacrifice, rats were tested for anxiety behaviors and endometriotic vesicles, and uterus were collected. Rats with endometriosis that received antalarmin significantly reduced the size (67% decrease) and number (30% decrease) of endometriotic vesicles. Antalarmin also prevented the increase in CRH and CRHR1 within endometriotic vesicles but not of glucocorticoid receptor. Behaviorally, endometriosis increased anxiety in the zero-maze test but antalarmin did not modify it. Our data provides the first demonstration for the effective use on CRHR1 antagonist for the treatment of endometriosis with promising effects for long-term therapy of this debilitating disease.

show abstract

Stress Management Affects Outcomes in the Pathophysiology of an Endometriosis Model

Cited by 33 publications

References 38 publications

Impact of Physical Activity on Pain Perception in an Animal Model of Endometriosis

Impact of Physical Activity on Pain Perception in an Animal Model of Endometriosis

Endometriosis Is Associated With a Shift in MU Opioid and NMDA Receptor Expression in the Brain Periaqueductal Gray

Antagonizing the corticotropin releasing hormone receptor 1 with antalarmin reduces the progression of endometriosis

Contact Info

Product

Resources

About