Stress Induction and Response, Inactivation, and Recovery of Vegetative Microorganisms by Pulsed Electric Fields

Schottroff, Felix; Krottenthaler, Anna; Jäger, Henry

doi:10.1007/978-3-319-32886-7_183

Cited by 6 publications

(16 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pulsed electric fields (PEFs) are used in a variety of different food processing applications, such as stress induction in microorganisms, mass transfer enhancement for food products or shelf life extension by microbial inactivation. Especially for the latter purpose, this technology bears a great potential, as it enables the efficient inactivation of vegetative microorganisms at distinctly lower process temperatures, in contrast to conventional heat treatments ( Schottroff et al, 2017 ).…”

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

“…Thus, at a constant voltage and pulse width, the energy per pulse decreases with a decreasing conductivity, due to a decreasing current. In order to obtain a constant total specific energy input, the frequency would have to be increased or the mass flow would have to be decreased ( Schottroff et al, 2017 ). Moreover, the pH of the treated product may exert strong effects on the inactivation efficiency by PEF.…”

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

“…Depending on microbial and media factors as well as treatment intensity, only one of these subpopulations might be present, but also several subpopulations can co-exist. During storage, the status of some of the individual populations can also merge into each other ( Schottroff et al, 2017 ). From a mechanistic point of view, a loss of the cell’s metabolic activity as well as the membrane’s barrier properties refers to the inactivated, e.g., dead, subpopulation, whereas an active state of these two microbial constituents refers to the living state.…”

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

“…If the membrane is perforated but the metabolic activity is still active, the sublethal condition is present. In this case, the injured population can subsequently develop into a dead or alive subpopulation ( Ulmer et al, 2002 ; Jaeger et al, 2009 ; Schottroff et al, 2017 ). The occurrence of sublethal injury depends on a variety of different factory, including product properties, microbial species, and treatment severity ( García et al, 2003 , 2005a ; Zhao et al, 2011 ).…”

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

“…It is well known that less severe preservation intensities result in non-injured, injured, and inactivated bacterial populations ( Silva et al, 2012 ; Wu, 2014 ; Schottroff et al, 2017 ), and the VBNC state can also be induced by preservation treatments ( Oliver, 2010 ; Ayrapetyan and Oliver, 2016 ). Thus, the detection of injured and VBNC bacteria is of distinct relevance for industrial food production, to avoid false-positive or false-negative results ( Wu, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Sublethal Injury and Viable but Non-culturable (VBNC) State in Microorganisms During Preservation of Food and Biological Materials by Non-thermal Processes

et al. 2018

Self Cite

View full text Add to dashboard Cite

The viable but non-culturable (VBNC) state, as well as sublethal injury of microorganisms pose a distinct threat to food safety, as the use of traditional, culture-based microbiological analyses might lead to an underestimation or a misinterpretation of the product’s microbial status and recovery phenomena of microorganisms may occur. For thermal treatments, a large amount of data and experience is available and processes are designed accordingly. In case of innovative inactivation treatments, however, there are still several open points with relevance for the investigation of inactivation mechanisms as well as for the application and validation of the preservation processes. Thus, this paper presents a comprehensive compilation of non-thermal preservation technologies, i.e., high hydrostatic pressure (HHP), pulsed electric fields (PEFs), pulsed light (PL), and ultraviolet (UV) radiation, as well as cold plasma (CP) treatments. The basic technological principles and the cellular and molecular mechanisms of action are described. Based on this, appropriate analytical methods are outlined, i.e., direct viable count, staining, and molecular biological methods, in order to enable the differentiation between viable and dead cells, as well as the possible occurrence of an intermediate state. Finally, further research needs are outlined.

show abstract

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Sublethal Injury and Viable but Non-culturable (VBNC) State in Microorganisms During Preservation of Food and Biological Materials by Non-thermal Processes

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

Nanosecond Pulsed Electric Field Lab‐on‐Chip Integrated in Super‐Resolution Microscope for Cytoskeleton Imaging

Havelka

Chafai

Krivosudský

et al. 2019

Adv Materials Technologies

View full text Add to dashboard Cite

Nanosecond pulsed electric field offers novel opportunities in bionanotechnology and biomedicine enabling ultrafast physical control of membrane, and protein‐based processes for the development of novel bionanomaterials and biomedical theranostic methods. However, the mechanisms of nanosecond pulsed electric field action at the nano‐ and molecular scale are not fully understood due to lack of appropriate research tools. In order to overcome this challenge, a technological platform for the exploration of these mechanisms in live biological samples is provided here. This paper describes step by step the proposed chip platform, including the design, fabrication, installation, and testing of the chip. The developed chip is capable of delivering hundreds of volts of nanosecond electric pulses compared to conventional chips using few volts. Moreover, the chip is fully integrated into a super‐resolution microscope. Later on, the chip function is demonstrated by affecting microtubule architecture in living cells. Therefore, the chip‐based technological advancement enables the assessment of pulsed electric field effects on bionanostructures and observing their effects in real‐time. The results contribute to the chip‐based high‐frequency bioelectronics technology for modulating the function of biological matter at the nanoscale level.

show abstract

Modulation of pulsed electric field induced oxidative processes in protein solutions by pro- and antioxidants sensed by biochemiluminescence

Červinková,

Vahalová,

Poplová

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Technologies based on pulsed electric field (PEF) are increasingly pervasive in medical and industrial applications. However, the detailed understanding of how PEF acts on biosamples including proteins at the molecular level is missing. There are indications that PEF might act on biomolecules via electrogenerated reactive oxygen species (ROS). However, it is unclear how this action is modulated by the pro- and antioxidants, which are naturally present components of biosamples. This knowledge gap is often due to insufficient sensitivity of the conventionally utilized detection assays. To overcome this limitation, here we employed an endogenous (bio)chemiluminescence sensing platform, which enables sensitive detection of PEF-generated ROS and oxidative processes in proteins, to inspect effects of pro-and antioxidants. Taking bovine serum albumin (BSA) as a model protein, we found that the chemiluminescence signal arising from its solution is greatly enhanced in the presence of as a prooxidant, especially during PEF treatment. In contrast, the chemiluminescence signal decreases in the presence of antioxidant enzymes (catalase, superoxide dismutase), indicating the involvement of both and electrogenerated superoxide anion in oxidation-reporting chemiluminescence signal before, during, and after PEF treatment. We also performed additional biochemical and biophysical assays, which confirmed that BSA underwent structural changes after treatment, with PEF having only a minor effect. We proposed a scheme describing the reactions leading from interfacial charge transfer at the anode by which ROS are generated to the actual photon emission. Results of our work help to elucidate the mechanisms of action of PEF on proteins via electrogenerated reactive oxygen species and open up new avenues for the application of PEF technology. The developed chemiluminescence technique enables label-free, in-situ and non-destructive sensing of interactions between ROS and proteins. The technique may be applied to study oxidative damage of other classes of biomolecules such as lipids, nucleic acids or carbohydrates.

show abstract

Stress Induction and Response, Inactivation, and Recovery of Vegetative Microorganisms by Pulsed Electric Fields

Cited by 6 publications

References 47 publications

Sublethal Injury and Viable but Non-culturable (VBNC) State in Microorganisms During Preservation of Food and Biological Materials by Non-thermal Processes

Sublethal Injury and Viable but Non-culturable (VBNC) State in Microorganisms During Preservation of Food and Biological Materials by Non-thermal Processes

Nanosecond Pulsed Electric Field Lab‐on‐Chip Integrated in Super‐Resolution Microscope for Cytoskeleton Imaging

Modulation of pulsed electric field induced oxidative processes in protein solutions by pro- and antioxidants sensed by biochemiluminescence

Contact Info

Product

Resources

About