Stress-Inducible Expression of an F-box Gene TaFBA1 from Wheat Enhanced the Drought Tolerance in Transgenic Tobacco Plants without Impacting Growth and Development

Kong, Xiangpei; Zhou, Shumei; Yin, Suhong; Zhao, Zhongxian; Han, Yangyang; Wang, Wei

doi:10.3389/fpls.2016.01295

Cited by 32 publications

(16 citation statements)

References 43 publications

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“…The results showed that this spraying rate had little influence on Microcoleus vaginatus and was suitable for their growth and amplification. Research found that low MDA content indicated that plants were under less stress, and high MDA content indicated more serious oxidative damage to the plant cell membrane [22,23], which was consistent with our results. Soluble protein makes up various antioxidant enzymes, and changes in their content reflect protein synthesis and the degradation of various information in the cell.…”

Section: Resultssupporting

confidence: 90%

Study of the Physiological Characteristics of Microcoleus vaginatus Combined with a Polymer Sand-Fixing Material Based on Attapulgite in the Laboratory

Lv¹,

Yan²,

Liu³

et al. 2020

Pol. J. Environ. Stud.

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Comprehensive sand-fixation technology has potential application prospects for desertification control and restoration. The feasibility of combining biological and chemical sand-fixation technology was explored by studying the growth state and physiological characteristics of Microcoleus vaginatus. Dried Microcoleus vaginatus and a polymer sand-fixation material based on attapulgite modified by 4 M H 2 SO 4 were combined as solids. The ratios of dried Microcoleus vaginatus to the polymer sand-fixation material by mass were 1:1, 1:2, 1:3 and 1:4. The amounts sprayed on the sand surface were 2 mg DW•cm-2 , 3 mg DW•cm-2 , 4 mg DW•cm-2 and 5 mg DW•cm-2. The results for chlorophyll a content, malondialdehyde content, soluble protein content and soluble sugar content showed that, under certain temperature and light conditions, the optimum environmental factors for the growth of Microcoleus vaginatus were a 1:3 ratio of dried Microcoleus vaginatus to the polymer sand-fixation material and a spraying rate of 3 mg DW•cm-2. The results could provide a theoretical basis for a new sand fixation technology; the method presented here serves as another approach for combating desertification in arid and semiarid areas.

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Section: Resultssupporting

confidence: 90%

Study of the Physiological Characteristics of Microcoleus vaginatus Combined with a Polymer Sand-Fixing Material Based on Attapulgite in the Laboratory

Lv¹,

Yan²,

Liu³

et al. 2020

Pol. J. Environ. Stud.

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“…Our previous studies showed that the wheat F-box gene TaFBA1 is involved in plant stress tolerance. It was shown that TaFBA1 overexpression improves drought, salt, and high-temperature stress tolerance of transgenic tobacco plants (Kong et al, 2016; Zhao et al, 2017; Li et al, 2018). We also observed that ABA could induce expression of the TaFBA1 gene in wheat (Zhou et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…We studied the functions of TaFBA1 in plant growth and stress tolerance by overexpressing it in tobacco. Study results showed that overexpression of TaFBA1 improves drought, salt, heat, and oxidative stress tolerance in transgenic tobacco plants (Zhou et al, 2014; Zhou et al, 2015; Kong et al, 2016; Zhao et al, 2017; Li et al, 2018). Results also showed that seed germination in transgenic tobacco that overexpresses TaFBA1 is less sensitive to ABA; stoma movement of TaFBA1-overexpressing tobacco was also insensitive to drought stress in our laboratory.…”

Section: Introductionmentioning

confidence: 99%

Wheat F-box Protein TaFBA1 Positively Regulates Plant Drought Tolerance but Negatively Regulates Stomatal Closure

Yang

et al. 2019

Front. Plant Sci.

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The phytohormone abscisic acid (ABA) regulates plant growth and development, as well as responses to various stresses, such as salt and drought. The wheat TaFBA1 gene, which encodes an F-box protein, was previously identified in our laboratory by homologous cloning. We previously found that TaFBA1 expression was induced by ABA and drought stress. In this study, wild-type (WT), TaFBA1 over-expressing (OEs), TaFBA1 homologous gene mutants, and TaFBA1 recovery (Rs) Arabidopsis plants were used. We found that the germination rate, the cotyledon greening rate, the root length, and the photosynthetic performance of TaFBA1 OE plants were better than those of WT under drought and ABA conditions, but mutant plants showed the opposite trend, and overexpression of TaFBA1 in mutants can recover their phenotype. In addition, TaFBA1 was found to be a negative regulator of ABA-induced stoma movement; mRNA transcription of certain ABA signaling-related genes was lower in TaFBA1 OE plants than in WT plants following ABA treatment. Further, we found that TaFBA1 can interact with RCAR1 (an ABA receptor) and ABI5. BiFC assay showed that TaFBA1 may interact with RCAR1 in the plasma membrane. In addition, accumulation of ROS and MDA in TaFBA1 OE plants was lower than that in the WT plants after ABA and drought treatments. Based on these results, we suggest that TaFBA1-regulated ABA insensitivity may be dependent on regulating ABA-mediated gene expression through interacting with RCAR1 and ABI5. Increased antioxidant competence and decreased ROS accumulation may be an important mechanism that underlies improved drought tolerance in TaFBA1 OE plants.

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“…In addition, our findings indicated that the ectopic expression of GmRR34 resulted in retarded growth in the transgenic plants under normal growth conditions ( Figure 1B-D). Previous studies also reported on similar phenotypic alteration in relation to improved drought tolerance/resistance when using constitutive 35S promoter to drive the expression of transgenes in various transgenic species, including Arabidopsis [40][41][42], tobacco [43,44], and tomato [45,46].…”

Section: Discussionmentioning

confidence: 64%

Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis

Nghia

Chuong

Hoang

et al. 2020

Plants

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Two-component systems (TCSs) have been identified as participants in mediating plant response to water deficit. Nevertheless, insights of their contribution to plant drought responses and associated regulatory mechanisms remain limited. Herein, a soybean response regulator (RR) gene RR34, which is the potential drought-responsive downstream member of a TCS, was ectopically expressed in the model plant Arabidopsis for the analysis of its biological roles in drought stress response. Results from the survival test revealed outstanding recovery ratios of 52%–53% in the examined transgenic lines compared with 28% of the wild-type plants. Additionally, remarkedly lower water loss rates in detached leaves as well as enhanced antioxidant enzyme activities of catalase and superoxide dismutase were observed in the transgenic group. Further transcriptional analysis of a subset of drought-responsive genes demonstrated higher expression in GmRR34-transgenic plants upon exposure to drought, including abscisic acid (ABA)-related genes NCED3, OST1, ABI5, and RAB18. These ectopic expression lines also displayed hypersensitivity to ABA treatment at germination and post-germination stages. Collectively, these findings indicated the ABA-associated mode of action of GmRR34 in conferring better plant performance under the adverse drought conditions.

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Stress-Inducible Expression of an F-box Gene TaFBA1 from Wheat Enhanced the Drought Tolerance in Transgenic Tobacco Plants without Impacting Growth and Development

Cited by 32 publications

References 43 publications

Study of the Physiological Characteristics of Microcoleus vaginatus Combined with a Polymer Sand-Fixing Material Based on Attapulgite in the Laboratory

Study of the Physiological Characteristics of Microcoleus vaginatus Combined with a Polymer Sand-Fixing Material Based on Attapulgite in the Laboratory

Wheat F-box Protein TaFBA1 Positively Regulates Plant Drought Tolerance but Negatively Regulates Stomatal Closure

Heterologous Expression of a Soybean Gene RR34 Conferred Improved Drought Resistance of Transgenic Arabidopsis

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