Stress induces depletion of Cdc25p and decreases the cAMP producing capability in Saccharomyces cerevisiae

Wang, Lili; Renault, Georges; Garreau, Hervé; Jacquet, Michel

doi:10.1099/mic.0.27162-0

Cited by 26 publications

(18 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Like Hsf1, Msn2/4 is hyperphosphorylated in response to heat shock, but this modification is inhibited by cAMP, suggesting that it is not mediated by PKA (Garreau et al 2000). Heat shock, oxidative and ethanol stress moderately reduce cAMP levels via destabilization of the Ras activator, Cdc25, leading to speculation that cAMP/PKA may yet be the nexus of ESR signaling and that additional phosphorylation events may act as subordinate modulators of the response (Wang et al 2004). Activation of the cell wall integrity pathway by heat shock has been reviewed elsewhere, but importantly is insulated from Hsf1 or Msn2/4 activation and is not affected by cAMP/PKA (Levin 2005).…”

Section: Control and Regulation Of The Heat Shock Responsementioning

confidence: 99%

The Response to Heat Shock and Oxidative Stress inSaccharomyces cerevisiae

2012

View full text Add to dashboard Cite

A common need for microbial cells is the ability to respond to potentially toxic environmental insults. Here we review the progress in understanding the response of the yeast Saccharomyces cerevisiae to two important environmental stresses: heat shock and oxidative stress. Both of these stresses are fundamental challenges that microbes of all types will experience. The study of these environmental stress responses in S. cerevisiae has illuminated many of the features now viewed as central to our understanding of eukaryotic cell biology. Transcriptional activation plays an important role in driving the multifaceted reaction to elevated temperature and levels of reactive oxygen species. Advances provided by the development of whole genome analyses have led to an appreciation of the global reorganization of gene expression and its integration between different stress regimens. While the precise nature of the signal eliciting the heat shock response remains elusive, recent progress in the understanding of induction of the oxidative stress response is summarized here. Although these stress conditions represent ancient challenges to S. cerevisiae and other microbes, much remains to be learned about the mechanisms dedicated to dealing with these environmental parameters.

show abstract

Section: Control and Regulation Of The Heat Shock Responsementioning

confidence: 99%

The Response to Heat Shock and Oxidative Stress inSaccharomyces cerevisiae

2012

View full text Add to dashboard Cite

show abstract

“…Although the NLS is controlled by four phosphorylation sites (Gorner et al 1998), we considered only one phosphorylation-dephosphorylation event in our simplified model because this is sufficient to couple the shuttling of Msn2 to the oscillatory dynamics of the cAMP-PKA system. The effect of stress is likely manifold: regardless of its precise nature-be it chemical, light emitted from the microscope, or carbon source limitation-we assumed that it controls the cAMP-PKA pathway through the inactivation of GEF (Wang et al 2004), and enhances the activity of phosphatases acting on Msn2, as can be deduced from the experimental results showing that Msn2 still responds to stress by entering the nucleus in the absence of any variation in cAMP (Garmendia-Torres et al 2007). …”

Section: Presentation Of the Deterministic Modelmentioning

confidence: 99%

Stochastic modelling of nucleocytoplasmic oscillations of the transcription factor Msn2 in yeast

Gonze

Jacquet

Goldbeter

2008

J. R. Soc. Interface.

View full text Add to dashboard Cite

Stress induces oscillatory nucleocytoplasmic shuttling of the transcription factor Msn2 in yeast. The subcellular localization of Msn2 is controlled by the cAMP-dependent protein kinase, PKA. Recent experimental observations corroborated by a deterministic computational model for the cAMP-PKA pathway in yeast suggest that the oscillatory dynamics of Msn2 results from the periodic activation of PKA associated with stress-induced oscillations in the level of cAMP. The model accounts for the occurrence of oscillations of Msn2 in a window bounded by two critical values of the stress intensity. In contrast to the rather irregular oscillatory behaviour observed within single yeast cells by means of fluorescence measurements, the deterministic model can only produce a regular pattern of oscillations. To investigate whether the experimentally observed variability could be explained by molecular noise due to the small number of molecules involved in the oscillatory mechanism, we examine a stochastic version of the model for periodic nucleocytoplasmic shuttling of Msn2 coupled to oscillations in the cAMP-PKA pathway. The results of stochastic simulations compare well to the irregular oscillations observed experimentally in the nucleocytoplasmic shuttling of Msn2 in individual yeast cells. The stochastic model retains the property of oscillations within a range bounded by two critical values of stress intensity. We determine the dynamic behaviour as a function of this control parameter and show that the effect of noise markedly depends on the distance from the bifurcation points in the domain of oscillatory behaviour. Finally, we assess the role played by thresholds due to zero-order ultrasensitivity in phosphorylation-dephosphorylation cycles, both in the cAMP-PKA pathway and in the reactions controlling nucleocytoplasmic shuttling of Msn2. In regard to these thresholds, stochastic simulations show that large-amplitude variations of Msn2 associated with large-amplitude oscillations in cAMP can occur outside the domain of sustained oscillations predicted by the deterministic approach.

show abstract

“…It is known that sexual development of Volvox is not only induced by the sex-inducer but also in response to heat and oxidative stress (Kirk and Kirk 1986;Nedelcu 2005;Nedelcu and Michod 2003) and also light plays a critical role in the success of this sexual induction (Starr et al 1980). In this regard, it is remarkable that cAMP has been shown to be involved in responses to heat and oxidative stress (Taminato et al 2002;Wang et al 2004). Provided that histidine kinase rhodopsins of V. carteri with adenyl cyclase activity can produce cAMP in a light-dependent manner in response to environmental signal inputs, it is likely that they participate in sexual development (Fig.…”

Section: Histidine Kinase Rhodopsinsmentioning

confidence: 99%