Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat

Vendruscolo, Eliane Cristina Gruszka; Schuster, Ivan; Pileggi, Marcos; Scapim, Carlos Alberto; Molinari, Hugo Bruno Correa; Marur, Celso Jamil; Vieira, Luiz Gonzaga Esteves

doi:10.1016/j.jplph.2007.05.001

Cited by 408 publications

(219 citation statements)

References 42 publications

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“…Similar results were found by Vendruscolo et al (2007) with transgenic plants of Triticum aestivum under water deficit.…”

Section: Stomatal Conductancesupporting

confidence: 86%

Consequences of the water deficit on water relations and symbiosis in Vigna unguiculata cultivars

Lobato¹,

Costa²,

Neto³

et al. 2009

PLANT SOIL ENVIRON

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The study aimed at evaluating and comparing changes provoked by the water deficit on water relations and nitrogen fixation in two Vigna unguiculata cultivars, as well as at indicating which cultivar is more tolerant under water deficiency. The experimental design used was entirely randomized in factorial scheme, with 2 cultivars (Pitiuba and Pérola) and 2 water regimes (control and stress). The parameters evaluated were the leaf relative water content, stomatal conductance, transpiration rate, nodule number, nodule dry matter, nitrate reductase enzyme activity, ureide concentration and leghemoglobin in nodule. The stomatal conductance of the Pitiuba and Pérola cultivars under water deficit were 0.20 and 0.01 mmol H 2 O/m 2 /s, respectively. The nitrate reductase activity of the plants under stress was significantly reduced in both cultivars. The leghemoglobin in the Pitiuba and Pérola cultivars under water stress had the concentrations of 58 and 41 g/kg dry matter, respectively. The parameters investigated in this study suggest that the Pitiuba cultivar under water deficit suffers from smaller changes, when compared with Pérola cultivar.

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“…Similar results were found by Vendruscolo et al (2007) with transgenic plants of Triticum aestivum under water deficit.…”

Section: Stomatal Conductancesupporting

confidence: 86%

Consequences of the water deficit on water relations and symbiosis in Vigna unguiculata cultivars

Lobato¹,

Costa²,

Neto³

et al. 2009

PLANT SOIL ENVIRON

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“…Water stress resulted in an increase in proline accumulation in sorghum (Yadav et al, 2005). The similar results were observed in sorghum (Zaifnejad et al, 1997;Al-Karaki et al, 1996) wheat (Nayyar, 2003;Vendruscolo et al, 2007); soybean (Heerden and Kruger, 2002). Proline content increased in both drought stress and with TDM treatments when compared to control.…”

Section: Prolinesupporting

confidence: 81%

Triazole compounds alters the antioxidant and osmoprotectant status in drought stressed Helianthus annuus L. plants

Rabert

Manivannan

Somasundaram

et al. 2014

Emir. J. Food Agric

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A pot-culture experiment was conducted to estimate the ameliorating effect of triazole compounds Hexaconazole, Tebuconazole and Propiconazole on drought stress in sunflower (Helianthus annuus L.). The plants were subjected to drought (DID) stress after four days of interval from the 30th day of sowing (DAS). One-day-interval irrigation was kept as control. The plant samples were collected and separated into root, stem and leaf for estimating the amino acid (AA), proline (PRO) and glycine betaine (GB) contents and the activities of antioxidant enzymes. Drought stress and triazole treatments increased AA, PRO and GB contents, superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) activities when compared to control. From the results of this investigation, it can be concluded that the application of triazole compounds caused a partial amelioration of the adverse effects of drought stress by its influence on antioxidant potentials in H. annuus.

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“…In previous studies, researchers have attempted to focus on P5CS expression patterns in higher plants and they found increasing levels of constitutive transcript abundance compared with the genes VaP5CS (de Carvalho et al, 2013), OsP5CS (Igarashi et al, 1997), PvP5CS (Chen et al, 2009), JcP5CS (Wang et al, 2011), AtP5CS1 (Zhuang et al, 2011) and MsP5CS1 (Fichman et al, 2014). Induction of transcript expression of P5CS gene has also been reported in response to abiotic stresses (Neffar et al, 2011) and it was found to encourage proline synthesis and improved stress tolerance (Vendruscolo et al, 2007;Patade et al, 2013). This however depends on regulation of its transcript expression and further metabolism to meet the plant's needs .…”

Section: Discussionmentioning

confidence: 95%

Producing Transgenic Thompson Seedless Grape (Vitis vinifera) Plants using Agrobacterium tumefaciens

Metwali¹,

Soliman²,

Almaghrabi³

et al. 2016

IJAB

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Under osmotic stress plant avoid it by different modification in their metabolisms such as increasing the synthesis of mRNA of delta 1-pyrroline-5-carboxylate synthase (P5CS), where P5CS is a target gene for increasing proline production and expect to improve the resistance to abiotic stress. To test this hypothesis, grape (Vitis vinifera L.) cv. Thompson Seedless was conducted to develop a protocol for high frequency regeneration and Agrobacterium mediated P5CS gene transfer. Callus induction was achieved by culture leaf explants on Nitsch and Nitsch (NN) basal medium including 2.0 mg L -1 2,4-di-chloro-phenoxy-aceticacid + 0.5 mg L -1 Thiadiazuron solidified with 2.5 g L -1 phytagel. While for in vitro proliferation of plant, the calli were cultured on NN medium supplemented with 3.0 mg L -1 Zeatin riboside + 0.5 mg L -1 Thiadiazuron. Agrobacterium-mediated transformation using the strain LB4404 harbouring the binary vector pBI121 with the P5CS gene under CaMV 35S promoter and the bar gene as a plant selectable marker was used for transforming grape explants. A P5CS specific band (2100 bp) was amplified from DNA extracted only from the transgenic grape plants and 24% for P5CS gene was positive for the bar gene. Over expression of the abiotic stress P5CS gene was enhanced synthesis of proline to over 6 times higher in transgenic plants compared to controls. The successful transformation of genetically diverse grape cv. Thompson Seedless indicated that the transformation system may have general application to an even wider range of grapes cultivars.

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Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat

Cited by 408 publications

References 42 publications

Consequences of the water deficit on water relations and symbiosis in Vigna unguiculata cultivars

Consequences of the water deficit on water relations and symbiosis in Vigna unguiculata cultivars

Triazole compounds alters the antioxidant and osmoprotectant status in drought stressed Helianthus annuus L. plants

Producing Transgenic Thompson Seedless Grape (Vitis vinifera) Plants using Agrobacterium tumefaciens

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