Stress-Induced Ribonucleases

Ivanov, Pavel; Anderson, Paul

doi:10.1007/978-3-642-21078-5_5

Cited by 6 publications

(6 citation statements)

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“…We have shown previously that mature cytoplasmic tRNAs are a source of a small RNAs which we termed tRNA-derived stress-induced RNAs (tiRNAs) 21 . tiRNAs are produced in response to various cellular stresses (e.g., oxidative stress, UV irradiation, and nutrient starvation) through RNA cleavage mediated by angiogenin (ANG), a stress-responsive member of the ribonuclease (RNase) A superfamily 22 , 23 . ANG cleaves mature tRNAs within the anticodon loop to produce 5ʹ and 3ʹ tiRNAs 24 .…”

Section: Introductionmentioning

confidence: 99%

Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs

et al. 2017

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RNA G-quadruplex (RG4) structures are involved in multiple biological processes. Recent genome-wide analyses of human mRNA transcriptome identified thousands of putative intramolecular RG4s that readily assemble in vitro but shown to be unfolded in vivo. Previously, we have shown that mature cytoplasmic tRNAs are cleaved during stress response to produce tRNA fragments that function to repress translation in vivo. Here we report that these bioactive tRNA fragments assemble into intermolecular RG4s. We provide evidence for the formation of uniquely stable tetramolecular RG4 structures consisting of five tetrad layers formed by 5ʹ-terminal oligoguanine motifs of an individual tRNA fragment. RG4 is required for functions of tRNA fragments in the regulation of mRNA translation, a critical component of cellular stress response. RG4 disruption abrogates tRNA fragments ability to trigger the formation of Stress Granules in vivo. Collectively, our data rationalize the existence of naturally occurring RG4-assembling tRNA fragments and emphasize their regulatory roles.

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Section: Introductionmentioning

confidence: 99%

Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs

et al. 2017

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“…The class I RNases has been reported that induced by Pi deficiency, senescence, wounding and osmotic stresses including drought [50]. RNase LE has a phosphatescavenging function in response to P i deficiency by hydrolyzing extracellular RNAs [51,52]. One of the consequences of drought stress in plants is the decrease in concentrations of nutrients especially P which caused by the decrease in P uptake of roots [53].…”

Section: Gene Regulatory Networkmentioning

confidence: 99%

Transcription Factors and microRNA Genes Regulatory Network Construction Under Drought Stress in Sesame (Sesamum indicum L.)

Baghery

Kazemitabar

Dehestani

et al. 2020

Preprint

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Background: Drought is one of the most common environmental stresses affecting crops yield and quality. Sesame is an important oilseed crop that most likely faces drought during its growth due to growing in semi-arid and arid areas. Plants responses to drought controlled by regulatory mechanisms. Despite this importance, there is little information about Sesame regulatory mechanisms against drought stress. Results: 458 drought-related genes were identified using comprehensive RNA-seq data analysis of two susceptible and tolerant sesame genotypes under drought stress. These drought-responsive genes were included secondary metabolites biosynthesis-related Like F3H, sucrose biosynthesis-related like SUS2, transporters like SUC2, and protectives like LEA and HSP families. Interactions between identified genes and regulators including TFs and miRNAs were predicted using bioinformatics tools and related regulatory gene networks were constructed. Key regulators and relations of Sesame under drought stress were detected by network analysis. TFs belonged to DREB (DREB2D), MYB (MYB63), ZFP (TFIIIA), bZIP (bZIP16), bHLH (PIF1), WRKY (WRKY30) and NAC (NAC29) families were found among key regulators. mRNAs like miR399, miR169, miR156, miR5685, miR529, miR395, miR396, and miR172 also found as key drought regulators. Furthermore, a total of 117 TFs and 133 miRNAs that might be involved in drought stress were identified with this approach. Conclusions: Most of the identified TFs and almost all of the miRNAs are introduced for the first time as potential regulators of drought response in Sesame. These regulators accompany with identified drought-related genes could be valuable candidates for future studies and breeding programs on Sesame under drought stress. Keywords: Sesamum indicum, Drought stress, Regulatory networks, miRNA, Transcription Factors.

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“…A variety of ribonucleases might degrade RNAs during chromatolysis such as those in the secreted, vertebrate ribonuclease family (Ivanov and Anderson, ; Nicholson, ) whose canonical member is bovine pancreatic RNase A (often known as Ribonuclease 1 or pancreatic RNase). In humans there are eight canonical Ribonucleases in this family.…”

Section: Which Ribonuclease(s) Might Cause Fragmentation Of Rough Endmentioning

confidence: 99%

“…Alternatively, perhaps ribonucleases that are associated with ribosomes (Datta and Ghosh, ; Bransgrove and Cosquer, ; Bates et al, ; Bates et al, ; Schoenberg and Maquat, ) become activated after injury (Allam et al, ). Ribonucleases are also known to change their subcellular distribution after injury or during stress (Ivanov and Anderson, ; Nicholson, ). Data obtained using non‐neural cells show that under conditions of stress, Ribonuclease 5 moves to the cytoplasm including to stress granules where it becomes activated and hemisects tRNAs into tiRNAs: this impairs translation of many proteins, although some proteins essential for cell survival continue to be manufactured (Pizzo et al, ).…”

Section: Which Ribonuclease(s) Might Cause Fragmentation Of Rough Endmentioning

confidence: 99%

Chromatolysis: Do injured axons regenerate poorly when ribonucleases attack rough endoplasmic reticulum, ribosomes and RNA?

Moon

2018

Developmental Neurobiology

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After axonal injury, chromatolysis (fragmentation of Nissl substance) can occur in the soma. Electron microscopy shows that chromatolysis involves fission of the rough endoplasmic reticulum. In CNS neurons (which do not regenerate axons back to their original targets) or in motor neurons or dorsal root ganglion neurons denied axon regeneration (e.g., by transection and ligation), chromatolysis is often accompanied by degranulation (loss of ribosomes from rough endoplasmic reticulum), disaggregation of polyribosomes and degradation of monoribosomes into dust-like particles. Ribosomes and rough endoplasmic reticulum may also be degraded in autophagic vacuoles by ribophagy and reticulophagy, respectively. In other words, chromatolysis is disruption of parts of the protein synthesis infrastructure. Whereas some neurons may show transient or no chromatolysis, severely injured neurons can remain chromatolytic and never again synthesize normal levels of protein; some may atrophy or die. Ribonuclease(s) might cause the following features of chromatolysis: fragmentation and degranulation of rough endoplasmic reticulum, disaggregation of polyribosomes and degradation of monoribosomes. For example, ribonucleases in the EndoU/PP11 family can modify rough endoplasmic reticulum; many ribonucleases can degrade mRNA causing polyribosomes to unchain and disperse, and they can disassemble monoribosomes; Ribonuclease 5 can control rRNA synthesis and degrade tRNA; Ribonuclease T2 can degrade ribosomes, endoplasmic reticulum and RNA within autophagic vacuoles; and Ribonuclease IRE1α acts as a stress sensor within the endoplasmic reticulum. Regeneration might be improved after axonal injury by protecting the protein synthesis machinery from catabolism; targeting ribonucleases using inhibitors can enhance neurite outgrowth and could be a profitable strategy in vivo. © 2018 Wiley Periodicals, Inc. Develop Neurobiol, 2018.

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Stress-Induced Ribonucleases

Cited by 6 publications

References 100 publications

Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs

Identification of functional tetramolecular RNA G-quadruplexes derived from transfer RNAs

Transcription Factors and microRNA Genes Regulatory Network Construction Under Drought Stress in Sesame (Sesamum indicum L.)

Chromatolysis: Do injured axons regenerate poorly when ribonucleases attack rough endoplasmic reticulum, ribosomes and RNA?

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