Stress-induced growth rate reduction restricts metabolic resource utilization to modulate osmo-adaptation time

Bonny, Alain R; Kochanowski, Karl; Diether, Maren; El-Samad, Hana

doi:10.1016/j.celrep.2021.108854

Cited by 16 publications

(21 citation statements)

References 45 publications

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“…Transient G1 arrest was obviously presented in the WT cells under copper exposure. Different stress responses show remarkable diversity in their sensing regulation; however, a common feature of any stress response is cell-cycle slowdown or arrest, resulting in a decrease in growth rate [ 41 ]. Copper exposure was found to be no exception, presenting cell-cycle arrest in this study.…”

Section: Resultsmentioning

confidence: 99%

Involvement of the High-Osmolarity Glycerol Pathway of Saccharomyces Cerevisiae in Protection against Copper Toxicity

Ren

Han

et al. 2022

Antioxidants

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Although essential for life, copper is also potentially toxic in concentrations that surpass physiological thresholds. The high-osmolarity glycerol pathway of yeast is the main regulator of adaptive responses and is known to play crucial roles in the responses to various stressors. The objective of this research is to determine whether the HOG pathway could be activated and to investigate the possible interplay of the HOG pathway and oxidative stress due to copper exposure. In this research, we demonstrate that copper could induce oxidative stress, including the elevated concentrations of reactive oxygen species (ROS) and malondialdehyde (MDA). Increased combination with GSH, increased intracellular SOD activity, and the up-regulation of relevant genes can help cells defend themselves against oxidative toxicity. The results show that copper treatment triggers marked and prolonged Hog1 phosphorylation. Significantly, oxidative stress generated by copper toxicity is essential for the activation of Hog1. Activated Hog1 is translocated to the nucleus to regulate the expressions of genes such as CTT1, GPD1, and HSP12, among others. Furthermore, copper exposure induced significant G1-phase cell cycle arrest, while Hog1 partially participated in the regulation of cell cycle progression. These novel findings reveal another role for Hog1 in the regulation of copper-induced cellular stress.

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Section: Resultsmentioning

confidence: 99%

Involvement of the High-Osmolarity Glycerol Pathway of Saccharomyces Cerevisiae in Protection against Copper Toxicity

Ren

Han

et al. 2022

Antioxidants

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“…Das pathogenetische Gewicht der Hyperosmolarität ist von der osmotischen Toleranz der Augenoberfläche abhängig, der Osmotoleranz, welche einer osmotischen Pufferfunktion gleichkommt. Diese auch als adaptive Stressantwort ("adaptive stress responses") bezeichnete Reaktion von Zellen sind zum Überleben und Erhalt der Homeostase erforderlich [36] Hyperosmolarer führt zum Auftreten pro-inflammatorischer Zytokine wie tumor necrosis factoralpha (TNF-alpha), Interleukin-8, und Interleukin-6 [37]. Entzündungsmechanismen sind wiederum Teil jener Kernmechanismen, die eine Verschlechterung des Trockenen Auges als Krankheitzustand vorantreiben [38].…”

Section: Hintergrund Und Einsichtenunclassified

Attrition and osmokinetics – Two concepts for the pathogenesis of dry eye disease

Setten

2021

Spektrum Augenheilkd.

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ZusammenfassungDie neuen Erkenntnisse der Pathophysiolgie des Trockenen Auges erkennen das Zusammenspiel von Tränen, Augenoberfläche und Lidoberfläche als eine funktionelle Einheit an. Der Begriff der Benetzungsfähigkeit der Tränen in Abhängigkeit der mikrotektonischen Anatomie der Augenoberfläche relativiert die Anforderungen an Träne und Tränenersatzmittel. Das Model der Attrition, welches die Effekte der friktionsneutralisierenden Kapazität des Tränenfilms, der Reibung und die Bedeutung der Mechanotransduktionskapazität des Epithels zusammenfasst, wird eingeführt und dessen pathophysiologische Bedeutung erläutert. Attrition und Benetzung bestimmen zusammen grundlegende pathophysiologische Vorgänge in der Augenoberfläche wie Aktivierung von Nerven (subjektive Beschwerden) sowie Entzündung und beeinflussen damit die Dynamik der Pathophysiologie, und den Übergang von vorübergehenden Beschwerden zu einer manifesten Erkrankung des Trockenen Auges. Die Betrachtung der Osmolarität als numerischer statischer Grenzwert zur alleinigen Diagnose des Trockenen Auges ist klinisch nicht haltbar. Das neue, dynamische Model der Osmokinetik, zeigt dagegen eine Alternative auf, in der die Tageschwankungen und die Beachtung des durchschnittlichen Osmolaritätsniveaus gröβere Bedeutung gewinnen und damit der eigentlichen pathophysiologischen Bedeutung der Osmolarität gerechter wird.

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“…In order to survive, the stressed cells slow down their growth and replication and shift the energetic flux to its cell maintenance by improving defense mechanisms (Ho et al 2018). Changes in its structure and or metabolism like thickening the cell wall, synthesis of enzymes to degrade inhibitors, and recovery of ionic balance are actions to attenuate the effect of inhibitors, adaptations that are passed to progeny (Ravishankar et al 2020;Bonny et al 2021;Liu et al 2021). These systematic responses could be regulated by distinct transcription factors, among others, favoring pathways activated under particular conditions (Hohmann and Mager 2003).…”

Section: Physiologic and Kinetic Analysis For Adapted And Parental Strainsmentioning

confidence: 99%

Induction of resistance mechanisms in Rhodotorula toruloides for growth in sugarcane hydrolysate with high inhibitor content

Lopes

Bonturi

Miranda

2021

Appl Microbiol Biotechnol

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The oleaginous yeast Rhodotorula toruloides is a potential lipid producer for biodiesel production. However, this yeast shows growth inhibition due to harmful compounds when cultivated in hemicellulose hydrolysate. Here, we present a comparative analysis of colony selection and heterologous adaptive laboratory enhancement (ALE) strategies for obtaining robust strains. We implemented these ALE strategies for R. toruloides in a culture medium containing sugarcane hemicellulose hydrolysate. Our comparison study showed that the strain obtained with heterogeneous ALE strategy (Rth) reached a µ max of 55% higher than the parental strain. It also exhibited higher biomass production (6.51 g/l) and lipid content (60%). ALE with colony selection strategy (Rtc) had a fitness gain in terms of shortening of the lag phase (9 h) when compared to Rth and parental strain (11.67, 12.33 h, respectively). When cultivated in Eucalyptus urograndis hemicellulose hydrolysate, the Rth strain achieved a high lipid content, 64%. Kinetics studies showed a strong effect of acetic acid as a repressor of xylose consumption during R. toruloides cultivation. Key points• Distinct adaptive laboratory strategies resulted in strains with different physiologies.• Heterologous adaptive laboratory enhancement provided the best results (fitness gain of 55% in µmax).• The Rth strain achieved a lipid content of 64.3% during cultivation in eucalyptus hemicellulose hydrolysate.

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Stress-induced growth rate reduction restricts metabolic resource utilization to modulate osmo-adaptation time

Cited by 16 publications

References 45 publications

Involvement of the High-Osmolarity Glycerol Pathway of Saccharomyces Cerevisiae in Protection against Copper Toxicity

Involvement of the High-Osmolarity Glycerol Pathway of Saccharomyces Cerevisiae in Protection against Copper Toxicity

Attrition and osmokinetics – Two concepts for the pathogenesis of dry eye disease

Induction of resistance mechanisms in Rhodotorula toruloides for growth in sugarcane hydrolysate with high inhibitor content

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