Stress-induced dendritic internalization and nuclear translocation of the neurokinin-3 (NK3) receptor in vasopressinergic profiles of the rat paraventricular nucleus of the hypothalamus

Miklos, Zachary; Flynn, Francis W.; Lessard, Andrée

doi:10.1016/j.brainres.2014.09.043

Cited by 7 publications

(3 citation statements)

References 65 publications

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“…In the present study, we confirm that, in the rat, and as reported previously (31), neurokinin receptor NK3R is densely expressed in vasopressin and oxytocin cells in the SON and PVN. We also confirm that, in the PVN, NK3R is expressed by most vasopressin cells but by only some oxytocin cells (32); however, in the SON, it appeared that all neurones, including all oxytocin cells, express NK3R. NKB was expressed in fibres in close juxtaposition with vasopressin cells in both the SON and PVN.…”

Section: Discussionsupporting

confidence: 81%

A Direct Neurokinin B Projection from the Arcuate Nucleus Regulates Magnocellular Vasopressin Cells of the Supraoptic Nucleus

Pineda

Sabatier

Ludwig

et al. 2016

J Neuroendocrinology

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Central administration of neurokinin B (NKB) agonists stimulates immediate early gene expression in the hypothalamus and increases secretion of vasopressin from the posterior pituitary through a mechanism that depends on the activation of neurokinin receptor 3 receptors (NK3R). Here we report that, in the rat, immunoreactivity for NK3R is expressed in magnocellular vasopressin and oxytocin neurones in the supraoptic nucleus (SON) and paraventricular nucleus (PVN) of the hypothalamus, and that NKB immunoreactivity is expressed in fibres in close juxtaposition with vasopressin neurones at both of these sites.Retrograde tracing in the rat showed that some NKB-expressing neurones in the arcuate nucleus project to the SON, and in mice, using an anterograde tracing approach, we found that kisspeptin-expressing neurones of the arcuate nucleus, which are known to co-express NKB, project to the SON and PVN. Finally, we show that i.c.v. injection of the NK3R 2 agonist senktide potently increases the electrical activity of vasopressin neurones in the SON in vivo with no significant effect detected on oxytocin neurons. The results suggest that NKBcontaining neurones in the arcuate nucleus regulate the secretion of vasopressin from magnocellular neurones in rodents, and we discuss the possible significance of this.

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Section: Discussionsupporting

confidence: 81%

A Direct Neurokinin B Projection from the Arcuate Nucleus Regulates Magnocellular Vasopressin Cells of the Supraoptic Nucleus

Pineda

Sabatier

Ludwig

et al. 2016

J Neuroendocrinology

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“…The reason for this diverse alteration of Kv7.3 levels between membrane and cytosolic fraction is not clear. It has been shown that acute stress alters receptor trafficking including NMDA and AMPA receptors (Yuen et al, 2011) as well as the neurokinin-3 receptor (Miklos et al, 2014). Thus, the inconsistent alteration of Kv7.3 levels may be caused by acute stress-induced protein degradation of Kv7.3 during the protein recycling process.…”

Section: Discussionmentioning

confidence: 99%

Acute stress diminishes M-current contributing to elevated activity of hypothalamic-pituitary-adrenal axis

et al. 2017

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Acute stress stimulates corticotrophin-releasing hormone (CRH)-expressing neurons in the hypothalamic paraventricular nucleus (PVN), which is an essential component of hypothalamic-pituitary-adrenal (HPA) axis. However, the cellular and molecular mechanisms remain unclear. The M-channel is a voltage-dependent K+ channel involved in stabilizing the neuronal membrane potential and regulating neuronal excitability. In this study, we tested our hypothesis that acute stress suppresses expression of Kv7 channels to stimulate PVN-CRH neurons and the HPA axis. Rat PVN-CRH neurons were identified by expressing enhanced green fluorescent protein driven by Crh promoter. Acute restraint stress attenuated the excitatory effect of Kv7 blocker XE-991 on the firing activity of PVN-CRH neurons and blunted the increase in plasma corticosterone (CORT) levels induced by microinjection of XE-991 into the PVN. Furthermore, acute stress significantly decreased the M-currents in PVN-CRH neurons and reduced PVN expression of Kv7.3 subunit in the membrane. In addition, acute stress significantly increased phosphorylated AMP-activated protein kinase (AMPK) levels in the PVN tissue. Intracerebroventricular injection of the AMPK inhibitor dorsomorphin restored acute stress-induced elevation of CORT levels and reduction of membrane Kv7.3 protein level in the PVN. Dorsomorphin treatment increased the M-currents and reduced the firing activity of PVN-CRH neurons in acutely stressed rats. Collectively, these data suggest that acute stress diminishes Kv7 channels to stimulate PVN-CRH neurons and the HPA axis potentially via increased AMPK activity.

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“…One of the important links of the neuroendocrine system of the hypothalamus is the paraventricular nucleus (PVN) (Toshinai et al, 2014;Bains et al, 2015). These structures are the vegetative centers of coordination of functions and consist of a number of neuronal populations, subnuclei, which differ in structural-functional features and the nature of the neural connections with various parts of the nervous and neuroendocrine systems (Miklos et al, 2014;. Disruptions of the light regime (prolonged illumination, constant darkness) cause immediate changes in the expression of the c-fos gene in the PVN.…”

Section: Introductionmentioning

confidence: 99%

The effects of melatonin on the activity of the c-fos gene in the structures of the hypothalamic paraventricular nucleus under prolonged lighting

Bulyk

Абрамов

Bulyk

et al. 2018

Regul. Mech. Biosyst.

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The effect of constant illumination as a stress factor on the state of the "early response" c-fos gene in the lateral magnocellular subnuclei of the paraventricular nucleus (lmPVN) of the hypothalamus of rats at different time intervals (day and night) has been studied. To identify c-Fos in histological sections of the hypothalamus, a secondary (indirect) immunofluorescence method is used. Expression of the product of this gene, the c-Fos protein, in animals kept under normal conditions of alternation of illumination and darkness, shows a clear-cut circadian character. There was a significant decrease in the area of the immunopositive sites of structures at night by 19.4% compared to daytime measurements. The average values of the areas of such immunopositive subnuclei sites varied also in subgroups of rats under light stimulation conditions, in which samples of lmPVN were selected for study at 2 pm and 2 am, but the differences between groups did not reach the level of reliability. Under conditions of light stress, the c-Fos concentration index decreased by 29.4% during the day, and by 16.5% at night in relation to similar values in the intact group. In animals that were in the standard light mode, the c-Fos protein concentration index was significantly higher during the day than at night. In the intact group, the night value of the c-Fos concentration index averaged only 71.5% of the daily value. In rats subjected to constant illumination, the day and night values of the c-Fos concentration index did not differ reliably. To correct stress-induced changes in the activity of the "early response" c-fos gene in the rat hypothalamus, melatonin (0.5 mg/kg body weight of the animal) was used. Injections of melatonin to rats exposed to constant illumination normalized the circadian rhythm of the area of the material immunoreactive to c-Fos, compared to the group of animals that did not receive melatonin. When using melatonin against the background of constant illumination, a sharp increase of the protein concentration in the lmPVN subnuclei of hypothalamus in daytime and less pronounced at night intervals was detected. Injections of melatonin to animals were also reflected in the daily dynamics of the index of c-Fos protein content in the lmPVN subnuclei under conditions of constant illumination. In the daytime observation period, the index was almost twice the experimental data on stressed animals without the introduction of the hormone, bringing it closer to normal. Prospects for further research in this sphere will allow us to better understand the place and role of subnuclei of paraventricular nuclei of the hypothalamus in the mechanisms of the formation of circadian rhythms of the rat brain.

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Stress-induced dendritic internalization and nuclear translocation of the neurokinin-3 (NK3) receptor in vasopressinergic profiles of the rat paraventricular nucleus of the hypothalamus

Cited by 7 publications

References 65 publications

A Direct Neurokinin B Projection from the Arcuate Nucleus Regulates Magnocellular Vasopressin Cells of the Supraoptic Nucleus

A Direct Neurokinin B Projection from the Arcuate Nucleus Regulates Magnocellular Vasopressin Cells of the Supraoptic Nucleus

Acute stress diminishes M-current contributing to elevated activity of hypothalamic-pituitary-adrenal axis

The effects of melatonin on the activity of the c-fos gene in the structures of the hypothalamic paraventricular nucleus under prolonged lighting

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