Stress Induced Callus Initiation and Shoot Regeneration in  Ovary Culture of Marigold (Tagetes spp)

eshwari, Than; Aswath, C.; Chaithra, N

doi:10.20546/ijcmas.2018.703.372

Cited by 2 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To achieve this, optimization of plant growth hormone concentrations is a key strategy. Thaneshwari, (2018), observed high callus induction (89.66%) when ovaries of marigold (Tagetes spp) were cultured on MS media fortified with 4.44 µM BAP+2.26 µM 2, 4-D. Similarly, Yarali and Yanmaz, (2017) induced callus efficiently from onion (Allium cepa L.) using a combination of 2, 4-D and BAP.…”

Section: The Effect Of Pgrs On Callus Inductionmentioning

confidence: 92%

Factors Affecting Callus Induction from Anther and Ovary of Okra (Abelmoschus esculentus L.)

Ibrahim¹,

Abbas²,

Aldabbagh³

et al. 2023

IJARe

View full text Add to dashboard Cite

Background: Okra [Abelmoschus esculentus (L.) Moench.] is a nutrient-rich vegetable crop widely grown in the tropics and sub-tropics mainly for its edible pods. The haploid technique has been used in plant breeding for the improvements of plants and to develop new varieties in relatively a short time. Hence, we have optimized several factors such as plant growth regulators (PGR), sucrose concentration, cold treatment, type of media and culture conditions for callus induction from the anther and ovary of okra (557 F1 hybrid). Methods: The flower buds of different sizes were collected to determine various stages of development and then subjected to cold pre-treatments. The explants were then cultured on various combinations of PGRs i.e., naphthyloxy acetic (NOA), Indole acetic acid (IAA), 2, 4-Dichlorophenoxy acetic acid (2, 4-D), Benzyl amino purine (BAP), isopentenyl adenine 2iP, Kinetin (KIN) and Thidiazuron (TDZ). Result: The optimum developmental stage of microspore for callus initiation was achieved from flower buds of okra and its size was about 11 mm long. Flower buds that emerged one week after the flowering showed significantly higher percentage of callus induction. The optimum stage for ovary and ovule culture were one or two days prior to anthesis and the flower buds stage was 35±1mm. In conclusion, our study investigated the effect of several factors that affects callus induction in okra and optimized cultured conditions for future haploid development for okra.

show abstract

Section: The Effect Of Pgrs On Callus Inductionmentioning

confidence: 92%

Factors Affecting Callus Induction from Anther and Ovary of Okra (Abelmoschus esculentus L.)

Ibrahim¹,

Abbas²,

Aldabbagh³

et al. 2023

IJARe

View full text Add to dashboard Cite

show abstract

“…4% sucrose concentration on MS media was found to be optimum for callus induction. Thaneshwari et al, (2018a) also studied the effect of stress on callus initiation and shoot regeneration in ovary culture of marigold and found that pretreatment of flower buds at 45 °C for 2hr resulted in best callus induction and shoot differentiation when cultured on MS media supplemented with 4 % sucrose, 4.44 µM BAP and 4.52 µM 2, 4-D and incubated in dark for 4 weeks at 25 °C. Minimum days to callus initiation and highest shoot differentiation rate were also reported when the flower buds pretreated at 4 °C for 24 hr shoot (Thaneshwari et al, 2018b).…”

Section: Marigoldmentioning

confidence: 99%