Stress induced biofilm formation in Propionibacterium acidipropionici and use in propionic acid production

Cavero-Olguin, Victor Hugo; Hatti‐Kaul, Rajni; Cardenas-Alegría, Oscar Victor; Gutierrez-Valverde, Marcelo; Alfaro-Flores, Adalid; Romero-Calle, Danitza Xiomara; Alvarez-Aliaga, Maria Teresa

doi:10.1007/s11274-019-2679-9

Cited by 19 publications

(14 citation statements)

References 57 publications

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“…which is abundant in MUF, was shown to boost production of EPS in other bacteria (Bauer et al, 2009;Sardari et al, 2017;Cirrincione et al, 2018). Adaptation to acid and salt has also been shown to increase EPS production in P. acidipropionici (Cavero-Olguin et al, 2019), as has the exposure to moderate acid pH (5.0) of Lactobacillus helveticus (Torino et al, 2001). Moreover, the marked impact of the growth medium on the biosynthesis of extractable surface layer proteins (SlpA, SlpB, SlpE, LspA), which was here enhanced in the cow's milk medium, may also play a role in morphological changes affecting P. freudenreichii.…”

Section: Discussionmentioning

confidence: 98%

Differential Adaptation of Propionibacterium freudenreichii CIRM-BIA129 to Cow’s Milk Versus Soymilk Environments Modulates Its Stress Tolerance and Proteome

et al. 2020

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Propionibacterium freudenreichii is a beneficial bacterium that modulates the gut microbiota, motility and inflammation. It is traditionally consumed within various fermented dairy products. Changes to consumer habits in the context of food transition are, however, driving the demand for non-dairy fermented foods, resulting in a considerable development of plant-based fermented products that require greater scientific knowledge. Fermented soymilks, in particular, offer an alternative source of live probiotics. While the adaptation of lactic acid bacteria (LAB) to such vegetable substrates is well documented, little is known about that of propionibacteria. We therefore investigated the adaptation of Propionibacterium freudenreichii to soymilk by comparison to cow’s milk. P. freudenreichii grew in cow’s milk but not in soymilk, but it did grow in soymilk when co-cultured with the lactic acid bacterium Lactobacillus plantarum. When grown in soymilk ultrafiltrate (SUF, the aqueous phase of soymilk), P. freudenreichii cells appeared thinner and rectangular-shaped, while they were thicker and more rounded in cow’s milk utltrafiltrate (MUF, the aqueous phase of cow milk). The amount of extractable surface proteins (SlpA, SlpB, SlpD, SlpE) was furthermore reduced in SUF, when compared to MUF. This included the SlpB protein, previously shown to modulate adhesion and immunomodulation in P. freudenreichii. Tolerance toward an acid and toward a bile salts challenge were enhanced in SUF. By contrast, tolerance toward an oxidative and a thermal challenge were enhanced in MUF. A whole-cell proteomic approach further identified differential expression of 35 proteins involved in amino acid transport and metabolism (including amino acid dehydrogenase, amino acid transporter), 32 proteins involved in carbohydrate transport and metabolism (including glycosyltransferase, PTS), indicating metabolic adaptation to the substrate. The culture medium also modulated the amount of stress proteins involved in stress remediation: GroEL, OpuCA, CysK, DnaJ, GrpE, in line with the modulation of stress tolerance. Changing the fermented substrate may thus significantly affect the fermentative and probiotic properties of dairy propionibacteria. This needs to be considered when developing new fermented functional foods.

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Section: Discussionmentioning

confidence: 98%

Differential Adaptation of Propionibacterium freudenreichii CIRM-BIA129 to Cow’s Milk Versus Soymilk Environments Modulates Its Stress Tolerance and Proteome

et al. 2020

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“…This article is protected by copyright. All rights reserved acidipropionici has been reported to produce extracellular polymers (Van Schalkwyk et al, 2003) and can be induced to form biofilms under stress (Cavero-Olguin et al, 2019). Pseudomonas aeruginosa is also known to form biofilms and indeed, sequences for alginate synthesis and regulatory genes, which are associated with biofilm formation (Hentzer et al, 2001), were found only in Building Control samples (unpublished data).…”

Section: Accepted Articlementioning

confidence: 96%

“…Laundry water is enriched in anionic surfactants. In a survey of four sites, Christova-Boal et al (1996) found laundry water to have a higher pH than shower/handwash water, likely due to greater amounts of detergents present. Laundry water also contained several orders of magnitude fewer total coliforms than shower/handwash water, although the abundances of faecal coliforms and faecal streptococci were in a similar range to the shower/ handwash water.…”

Section: Laundry Watermentioning

confidence: 99%

“…The majority of the metagenomics sequences from the building control samples were assigned to the Acidipropionibacterium, which contain known fermenters of dairy and have actually been used for fermentation of sewage sludge (Li et al 2013). Additionally, A. acidipropionici has been reported to produce extracellular polymers (Van Schalkwyk et al 2003) and can be induced to form biofilms under stress (Cavero-Olguin et al 2019). Pseudomonas aeruginosa is also known to form biofilms, and indeed, sequences for alginate synthesis and regulatory genes, which are associated with biofilm formation (Hentzer et al 2001), were found only in building control samples (S. Keely, unpublished data).…”

Section: Greywater Treatment Systemsmentioning

confidence: 99%

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Human‐ and infrastructure‐associated bacteria in greywater

et al. 2021

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Greywater, the wastewater from sinks, showers and laundry, is an understudied environment for bacterial communities. Most greywater studies focus on quantifying pathogens, often via proxies used in other wastewater, like faecal indicator bacteria; there is a need to identify more greywater-appropriate surrogates, like Staphylococcus sp. Sequencing-based studies have revealed distinct communities in different types of greywater as well as in different parts of greywater infrastructure, including biofilms on pipes, holding tanks and filtration systems. The use of metagenomic sequencing provides high resolution on both the taxa and genes present, which may be of interest in cases like identifying pathogens and surrogates relevant to different matrices, monitoring antibiotic resistance genes and understanding metabolic processes occurring in the system. Here, we review what is known about bacterial communities in different types of greywater and its infrastructure. We suggest that wider adoption of environmental sequencing in greywater research is important because it can describe the entire bacterial community along with its metabolic capabilities, including pathways for removal of nutrients and organic materials. We briefly describe a metagenomic dataset comparing different types of greywater samples in a college dormitory building to highlight the type of questions these methods can address. Metagenomic sequencing can help further the understanding of greywater treatment for reuse because it allows for identification of new pathogens or genes of concern.

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“…Recent studies have shown that the production of biofilm and exopolysaccharides (EPS) facilitates immobilization of Propionibacterium freudenreichii and Propionibacterium acidipropionici . The formation of biofilm and EPS can be induced by triggering factors such as NaCl and citric acid ( 102 ).…”

Section: Process Parameters Influencing Microbial Production and Prodmentioning

confidence: 99%

Propionic Acid

Ranaei

Pilevar

Khaneghah

et al. 2020

Food technol. biotechnol. (Online)

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During the past years, there has been a growing interest in the bioproduction of propionic acid by Propionibacterium. One of the major limitations of the existing models lies in their low productivity yield. Hence, many strategies have been proposed in order to circumvent this obstacle. This article provides a comprehensive synthesis and review of important biotechnological aspects of propionic acid production as a common ingredient in food and biotechnology industries. We first discuss some of the most important production processes, mainly focusing on biological production. Then, we provide a summary of important propionic acid producers, including Propionibacterium freudenreichii and Propionibacterium acidipropionici, as well as a wide range of reported growth/production media. Furthermore, we describe bioprocess variables that can have impact on the production yield. Finally, we propose methods for the extraction and analysis of propionic acid and put forward strategies for overcoming the limitations of competitive microbial production from the economical point of view. Several factors influence the propionic acid concentration and productivity such as culture conditions, type and bioreactor scale; however, the pH value and temperature are the most important ones. Given that there are many reports about propionic acid production from glucose, whey permeate, glycerol, lactic acid, hemicelluloses, hydrolyzed corn meal, lactose, sugarcane molasses and enzymatically hydrolyzed whole wheat flour, only few review articles evaluate biotechnological aspects, i.e. bioprocess variables.

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Stress induced biofilm formation in Propionibacterium acidipropionici and use in propionic acid production

Cited by 19 publications

References 57 publications

Differential Adaptation of Propionibacterium freudenreichii CIRM-BIA129 to Cow’s Milk Versus Soymilk Environments Modulates Its Stress Tolerance and Proteome

Differential Adaptation of Propionibacterium freudenreichii CIRM-BIA129 to Cow’s Milk Versus Soymilk Environments Modulates Its Stress Tolerance and Proteome

Human‐ and infrastructure‐associated bacteria in greywater

Propionic Acid

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