Stress erythropoiesis in atherogenic mice

Abstract

Bone marrow erythropoiesis is mainly homeostatic and a demand of oxygen in tissues activates stress erythropoiesis in the spleen. Here, we show an increase in the number of circulating erythrocytes in apolipoprotein E−/− mice fed a Western high-fat diet, with similar number of circulating leukocytes and CD41+ events (platelets). Atherogenic conditions increase spleen erythropoiesis with no variations of this cell lineage in the bone marrow. Spleens from atherogenic mice show augmented number of late-stage erythroblasts and biased differentiation of progenitor cells towards the erythroid cell lineage, with an increase of CD71+CD41CD34−CD117+Sca1−Lin− cells (erythroid-primed megakaryocyte-erythroid progenitors), which is consistent with the way in which atherogenesis modifies the expression of pro-erythroid and pro-megakaryocytic genes in megakaryocyte-erythroid progenitors. These data explain the transiently improved response to an acute severe hemolytic anemia insult found in atherogenic mice in comparison to control mice, as well as the higher burst-forming unit-erythroid and colony forming unit-erythroid capacity of splenocytes from atherogenic mice. In conclusion, our work demonstrates that, along with the well stablished enhancement of monocytosis during atherogenesis, stress erythropoiesis in apolipoprotein E−/− mice fed a Western high fat diet results in increased numbers of circulating red blood cells.

Hypothyroidism confers tolerance to cerebral malaria

“…S4C). Among the different late erythroblasts, gated as described in ( 32 ) and fig. S5 (A and B), infected hypothyroid mice showed a reduced number of Ery B with respect to the euthyroid mice, and Ery C supported in both groups the major increase at day 6 after infection (fig.…”

“…Unstained cells were used as a negative control to establish the flow cytometer voltage settings, and single-color positive controls were used to adjust compensation. To identify the different splenic cells, without treatment with lysis solution, cells were subjected to flow cytometry analysis as described ( 32 , 64 ). The absolute number of cells was calculated by adding Perfect-Count Microspheres (CYTPCM-100, Cytognos) to the flow cytometry samples.…”

The immunoregulatory function of peripheral blood CD71+ erythroid cells in systemic-onset juvenile idiopathic arthritis

“…Stress erythropoiesis resembles fetal erythropoiesis in many ways 39 . In a murine model of stress erythropoiesis, monocytes were recruited to the spleen and formed an erythropoiesis niche, called erythroblastic islands, with erythroid progenitors 40 , 41 . Splenic erythroid progenitors share surface markers with burst-forming unit-erythroid progenitors (BFU-Es) from murine fetal liver 42 .…”

New Clues to Cardiovascular Disease: Erythrocyte Lifespan

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