Stress down regulates milk yield in cows by plasmin induced β-casein product that blocks K+ channels on the apical membranes

Silanikove, Nissim; Shamay, Avi; Shinder, D.; Moran, Arie

doi:10.1016/s0024-3205(00)00808-0

Cited by 91 publications

(98 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The quality of milk for cheese production was tested by measuring curd firmness (CF) and rennet clotting time (RCT) using an Optigraph (Ysebaret, Frepillon, France) . Additional sub-sets of milk samples were defatted under cold conditions and analyzed according to previously described procedures: concentrations of casein, whey proteins, proteose peptones, lactoferrin (enzyme-linked immunosorbent assay -ELISA), albumin (ELISA), Na 1 and K 1 , activities of PA, PLG and PL (Silanikove et al, 2000 and2009b), activities of XO, LPO and catalase and the concentrations of nitrite (fluorometrically by the DAN reagent) and nitrate (colorimetrically by the Griess reaction). The formation of potential harmful products in milk was assessed by analyzing the concentration of Ntyr in whey proteins by ELISA, the concentration of carbonyls in whey protein and the formation of lipid peroxides (Lpx) ion milk lipids by colorimetric reactions .…”

Section: Analytical Proceduresmentioning

confidence: 99%

“…The simultaneous reduction in milk quality and milk secretion in infected glands is related to the activation of plasminogen activator (PA), plasminogen (PG) and plasmin (PL)-derived active peptides from milk b-casein (Silanikove et al, 2000(Silanikove et al, and 2009aShamay et al, 2002 and. Enzymes linked to the metabolism of nitric oxide (NO) have also been shown recently to considerably affect the milk composition of inflamed mammary glands (Silanikove et al, , 2009a(Silanikove et al, and 2009b.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Silanikove

Rauch-Cohen

Shapiro

et al. 2012

Animal

View full text Add to dashboard Cite

The aim of this work was to study the effects of mastitis induced by intramammary lipopolysaccharide (LPS) challenge on milk oxidative stability, as well as to understand the underlying biochemical processes that cause such changes. LPS challenge was associated with nitric oxide burst from the surrounding mammary epithelial cells and consequently induced nitrosative stress that was induced by the formation of NO 2 d from nitrite by lactoperoxidase. This response was associated with an , 3-fold increased formation of hazardous compounds: nitrotyrosines, carbonyls and lipid peroxides. We sustained the involvement of xanthine oxidase as a major source of hydrogen peroxide. In consistent with previous findings, catalase has been shown to play a major role in modulating the nitrosative stress by oxidizing nitrite to nitrate. The current hygienic quality criteria cannot detect mixing of low-quality milk, such as milk with high somatic cells, and nitrite with high-quality milk. Thus, development of an improved quality control methodology may be important for the production of high-quality milk.Keywords: milk, cow, mastitis, NO and NO 2 d, plasmin system Implication In this study, we show that intramammary treatment with lipopolysaccharide induced nitric oxide burst that was associated with nitrosative stress that reflected the formation of hazardous compounds in milk: nitrotyrosines, carbonyls and lipid peroxides. These changes were associated with impairment in milk quality. Currently, very little is known about the presence of oxidative substances in commercial milk and their industrial and health implications. As mastitis is a prevalent problem in the dairy industry, this study stresses the importance of gaining further information on the effect of different forms of mastitis on milk composition.

show abstract

Section: Analytical Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Silanikove

Rauch-Cohen

Shapiro

et al. 2012

Animal

View full text Add to dashboard Cite

show abstract

“…In a recent study, Silanikove et al [71] showed that the fragment of b-CN (f1-18) resulting from proteolysis is involved in the decrease in milk secretion, which could be caused by the blocking of K + ducts on the apical side of the epithelial cells.…”

Section: A Decrease In Synthesismentioning

confidence: 99%

Polymorphonuclear proteolytic activity and milk composition change

Roux¹,

Laurent²,

Moussaoui³

2003

Vet. Res.

117

View full text Add to dashboard Cite

-Relationships between Quarter Milk Cell Count (QMCC) and Tank Milk Cell Count (TMCC) with milk biochemical and technological parameters in milk and dairy products were investigated. All parameters measured were affected by the increase of TMCC and QMCC between 0 and 600 000 cells/mL. The variable effect of lactation stage which is different for different authors, is discussed. The three mechanisms, measured during the inflammation of the udder, implicated in the modification of milk quality are described (a decrease in synthesis, a decrease in the milk barrier permeability and an increase in proteolytic activities). The direct effect of plasmin in caseinolysis is well known; the specific role of the increase of somatic cells (especially PMN) in the modification of milk quality is described. Several specific proteolytic activities of PMN are described and the impact of these activities on caseinolysis is evaluated. Two hypothetical mechanisms of caseinolysis by PMN are suggested and a synthetic scheme of the role of plasmin, bacteria and somatic cells in caseinolysis is discussed. caseinolysis / mastitis / milk / PMN proteases / SCC

show abstract

“…However, β-casein (fraction 1-28), a phosphopeptide peptide derived from mild activation of plasmin activity, reduces milk secretion by a process associated with its ability to block potassium channels in the apical membranes of MEC [62]. Furthermore, a separate mechanism that regulates TJ during milk stasis implicates local synthesis of serotonin by the mammary epithelium in a negative feedback, autocrine-paracrine loop that opposes endocrine stimulation of milk production and secretion in lactating mice [63] and dairy cows [12,13].…”

Section: Discussionmentioning

confidence: 99%

Induced Physical Distension of Rat Mammary Glands Accelerates the Onset of Apoptosis and Involution

Phyn¹,

Davis²,

Dobson³

et al. 2017

J Cell Signal

View full text Add to dashboard Cite

Objective: Weaning is a process that results in a reduction in milk secretion, an increase in mammary epithelial cell (MEC) apoptosis, and involution of the mammary gland. The local mechanisms initiating MEC apoptosis and involution are unclear, although the physical morphology of the MEC may influence cell-cell and cell-extracellular matrix communication and thus may alter function. This study examined the effect of physical distension of alveoli on the early molecular events that occur at the onset of involution of rat mammary glands. Methods:Mammary tissue was collected post-mortem from lactating Sprague-Dawley rats at 0, 1, 3, and 6 h (n=6 per time point) following acute physical distension of an inguinal gland with isosmotic sucrose solution (0.8 ml; equivalent to ~6 h worth of milk accumulation) via the teat canal followed by sealing with adhesive (infused). The remaining teats on each rat were either left unsealed for pups to suckle (control), or were sealed to induce milk accumulation and mammary engorgement (engorged). Results:There was a low number of positive in situ-end labeled (ISEL) nuclei in suckled control glands indicating they had a low number of cells with fragmented DNA or were apoptotic. However, there was a greater number of ISEL nuclei in sucrose-infused and milk-engorged teat-sealed glands by 1 and 6 h, respectively, such that these changes were accelerated by sucrose infusion, compared with milk accumulation alone. The timing of the decline in abundance of β1-integrin (cell-extracellular matrix protein) and occludin (tight junction protein), and increase in abundance of the activated apoptotic marker signal transducer and activator of transcription factor-3 (pSTAT3) protein were also accelerated by sucrose infusion. Conclusion:Physical distension by sucrose infusion accelerated the onset of the first stage of mammary apoptosis and involution, supporting a role for mechanotransduction during these processes.

show abstract

Stress down regulates milk yield in cows by plasmin induced β-casein product that blocks K+ channels on the apical membranes

Cited by 91 publications

References 23 publications

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Polymorphonuclear proteolytic activity and milk composition change

Induced Physical Distension of Rat Mammary Glands Accelerates the Onset of Apoptosis and Involution

Contact Info

Product

Resources

About