Stress Biology of Yeasts and Fungi

Kitagaki, Hiroshi

doi:10.1007/978-4-431-55248-2

Cited by 8 publications

(1 citation statement)

References 337 publications

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“…Yeast cells are constantly exposed to various environmental stresses, either in nature or in industrial applications, such as nutrient limitation, temperature, oxidative, osmotic, chemical, and acid stresses, etc. ( Boer et al, 2003 ; Morano et al, 2012 ; Takagi and Kitagaki, 2015 ; Song et al, 2021 ). To cope with these adverse situations, yeast cells have evolved remarkably sophisticated and flexible transcriptional regulatory networks that allow them to survive and thrive in harsh conditions, accompanying the induction of stress defense as well as alterations in cell growth and physiological activities ( Gasch et al, 2000 ; Lopez-Maury et al, 2008 ; Ho and Gasch, 2015 ; Taymaz-Nikerel et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

A Hierarchical Transcriptional Regulatory Network Required for Long-Term Thermal Stress Tolerance in an Industrial Saccharomyces cerevisiae Strain

Gan

Lin

et al. 2022

Front. Bioeng. Biotechnol.

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Yeast cells suffer from continuous and long-term thermal stress during high-temperature ethanol fermentation. Understanding the mechanism of yeast thermotolerance is important not only for studying microbial stress biology in basic research but also for developing thermotolerant strains for industrial application. Here, we compared the effects of 23 transcription factor (TF) deletions on high-temperature ethanol fermentation and cell survival after heat shock treatment and identified three core TFs, Sin3p, Srb2p and Mig1p, that are involved in regulating the response to long-term thermotolerance. Further analyses of comparative transcriptome profiling of the core TF deletions and transcription regulatory associations revealed a hierarchical transcriptional regulatory network centered on these three TFs. This global transcriptional regulatory network provided a better understanding of the regulatory mechanism behind long-term thermal stress tolerance as well as potential targets for transcriptome engineering to improve the performance of high-temperature ethanol fermentation by an industrial Saccharomyces cerevisiae strain.

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Section: Introductionmentioning

confidence: 99%

A Hierarchical Transcriptional Regulatory Network Required for Long-Term Thermal Stress Tolerance in an Industrial Saccharomyces cerevisiae Strain

Gan

Lin

et al. 2022

Front. Bioeng. Biotechnol.

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Alpha-ketoglutarate enhances freeze–thaw tolerance and prevents carbohydrate-induced cell death of the yeast Saccharomyces cerevisiae

et al. 2017

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Stress resistance and fermentative capability are important quality characteristics of baker's yeast. In the present study, we examined protective effects of exogenous alpha-ketoglutarate (AKG), an intermediate of the tricarboxylic acid cycle and amino acid metabolism, against freeze-thaw and carbohydrate-induced stresses in the yeast Saccharomyces cerevisiae. Growth on AKG-supplemented medium prevented a loss of viability and improved fermentative capacity of yeast cells after freeze-thaw treatment. The cells grown in the presence of AKG had higher levels of amino acids (e.g., proline), higher metabolic activity and total antioxidant capacity, and higher activities of catalase, NADP-dependent glutamate dehydrogenase and glutamine synthase compared to control ones. Both synthesis of amino acids and enhancement of antioxidant system capacity could be involved in AKG-improved freeze-thaw tolerance in S. cerevisiae. Cell viability dramatically decreased under incubation of stationary-phase yeast cells in 2% glucose or fructose solutions (in the absence of the other nutrients) as compared with incubation in distilled water or in 10 mM AKG solution. The decrease in cell viability was accompanied by acidification of the medium, and decrease in cellular respiration, aconitase activity, and levels of total protein and free amino acids. The supplementation with 10 mM AKG effectively prevented carbohydrate-induced yeast death. Protective mechanisms of AKG could be associated with the intensification of respiration and prevention of decreasing protein level as well as with direct antioxidant AKG action.

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Pre-fermentation of malt whisky wort using Lactobacillus plantarum and its influence on new-make spirit character

et al. 2020

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Stress Biology of Yeasts and Fungi

Cited by 8 publications

References 337 publications

A Hierarchical Transcriptional Regulatory Network Required for Long-Term Thermal Stress Tolerance in an Industrial Saccharomyces cerevisiae Strain

A Hierarchical Transcriptional Regulatory Network Required for Long-Term Thermal Stress Tolerance in an Industrial Saccharomyces cerevisiae Strain

Alpha-ketoglutarate enhances freeze–thaw tolerance and prevents carbohydrate-induced cell death of the yeast Saccharomyces cerevisiae

Pre-fermentation of malt whisky wort using Lactobacillus plantarum and its influence on new-make spirit character

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