The last decades have been characterised by new information and communication technologies (ICT) and the associated digital transformation of work and working conditions. Swiss stress monitoring studies indicate that the process of digitalisation has led to an increase in work-related stress. Nevertheless, validated self-report instruments measuring stressors and resources from digitalisation are not yet widely used in Switzerland. Thus, the aim of this study was to identify specific ICT-related resources and stressors and to develop a questionnaire that is broadly applicable in the population of Swiss employees. First, we identified existing ICT-specific constructs and revised their definitions to ensure content validity. Experts then rated the comprehensibility and content validity of these scales. Subsequently, 375 individuals participated in an online survey between November 2020 and January 2021 to evaluate reliability and construct validity. The final questionnaire comprises the three resources involvement facilitation, ICT control, and ICT resources and upgrades, as well as the stressor telepressure. The four scales comprise a total of 16 items and showed good reliabilities as well as measurement invariance regarding gender and importance of ICTs for one’s work. Using structural equation modelling, criterion validity was demonstrated with the prediction of exhaustion, well-being, general health, as well as job satisfaction, affective commitment, and intentions to quit. Furthermore, the new scales explained additional variance beyond general task-related resources and stressors. The new ICT Resources and Stressors Scale is recommended for use in Swiss stress monitoring studies.