Stress and metabolic regulation in Drosophila

Clark, Andrew G.

doi:10.1007/978-3-0348-8882-0_7

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Our study identified a cytochrome P450 gene as a downstream factor for PKA signaling in TPB. Given the potential relationship between temperature and stress related genes [32]–[34], this work could provide clues to understanding the relationship between stress response and temperature preference behavior. Although we focused on characterizing the function of cyp6a17 in this study, our genome-wide screen identified many other genes that showed strong response to either PKA CA or PKA DN (Table S1).…”

Section: Discussionmentioning

confidence: 99%

Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila

Kang

Kim²,

Choi

2011

PLoS ONE

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Perception of temperature is an important brain function for organisms to survive. Evidence suggests that temperature preference behavior (TPB) in Drosophila melanogaster, one of poikilothermal animals, is regulated by cAMP-dependent protein kinase (PKA) signaling in mushroom bodies of the brain. However, downstream targets for the PKA signaling in this behavior have not been identified. From a genome-wide search for the genes regulated by PKA activity in the mushroom bodies, we identified the cyp6a17 Cytochrome P450 gene as a new target for PKA. Our detailed analysis of mutants by genetic, molecular and behavioral assays shows that cyp6a17 is essential for temperature preference behavior. cyp6a17 expression is enriched in the mushroom bodies of the adult brain. Tissue-specific knockdown and rescue experiments demonstrate that cyp6a17 is required in the mushroom bodies for normal temperature preference behavior. This is the first study, to our knowledge, to show PKA-dependent expression of a cytochrome P450 gene in the mushroom bodies and its role as a key factor for temperature preference behavior. Taken together, this study reveals a new PKA-Cytochrome P450 pathway that regulates the temperature preference behavior.

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Section: Discussionmentioning

confidence: 99%

Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila

Kang

Kim²,

Choi

2011

PLoS ONE

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“…To our knowledge, no mutagenesis screen has been conducted in Drosophila with the specific purpose of looking for genes that affect obesity or triglyceride levels in Drosophila. However, quantitative trait loci (QTL) analyses and multigenerational selection experiments have been performed in Drosophila to determine the genetic basis for the natural variation in triglyceride levels (24,25,90). Also, mutations that affect lipid storage as secondary phenotypes were identified in massive screens for mutations that cause patterning defects in Drosophila embryos, such as those conducted in the early 1980s and before for which Edward Lewis, Eric Wieschaus, and Christiane Nüsslein-Volhard shared the 1995 Nobel Prize in Physiology or Medicine (67,93,139).…”

Section: Classical Genetic Screensmentioning

confidence: 99%

“…A caveat to performing screens using the isogenic deficiency lines is that single gene mutations often have very different phenotypes, depending on genetic backgrounds (see, for example, Reference 73). This suggests that focusing on a single isogenic strain will give some false negatives, and QTL-type approaches are valid and essential alternatives (24,90).…”

Section: Whole-genome Deficiency Screensmentioning

confidence: 99%

DROSOPHILA NUTRIGENOMICS CAN PROVIDE CLUES TO HUMAN GENE-NUTRIENT INTERACTIONS

et al. 2005

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Nutrigenomics refers to the complex effects of the nutritional environment on the genome, epigenome, and proteome of an organism. The diverse tissue- and organ-specific effects of diet include gene expression patterns, organization of the chromatin, and protein post-translational modifications. Long-term effects of diet range from obesity and associated diseases such as diabetes and cardiovascular disease to increased or decreased longevity. Furthermore, the diet of the mother can potentially have long-term health impacts on the children, possibly through inherited diet-induced chromatin alterations. Drosophila is a unique and ideal model organism for conducting nutrigenomics research for numerous reasons. Drosophila, yeast, and Caenorhabditis elegans all have sophisticated genetics as well as sequenced genomes, and researchers working with all three organisms have made valuable discoveries in nutrigenomics. However, unlike yeast and C. elegans, Drosophila has adipose-like tissues and a lipid transport system, making it a closer model to humans. This review summarizes what has already been learned in Drosophila nutrigenomics (with an emphasis on lipids and sterols), critically evaluates the data, and discusses fruitful areas for future research.

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“…These control lines are important because balancer chromosomes do not preclude the possibility of rare double recombinants, and heterogeneity among control lines may have been generated by deletions caused by P-element excisions. The lines were maintained at a constant adult density in 95 mm shell vials on Carolina 4-24 medium in incubators with a 12 h light/dark cycle at 25 ~ Quantitative PCR P-element copy number was determined by quantitative PCR (Clark, Wang & Hulleberg, 1995). Oligonucleotide primers were designed for the sense strand of the first exon, and the antisense strand of the first intron and the second exon of the white gene.…”

Section: P-element Transpositionmentioning

confidence: 99%

Probing the evolution of senescence inDrosophila melanogaster withP-element tagging

Clark

Guadalupe

1995

Genetica

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Natural populations host a wealth of genetic variation in longevity and age-specific schedules of reproduction. This variation provides critical information for inferring the evolutionary origin of senescence. Patterns of mutational effects on age-specific fecundity and survival provide additional insight to distinguish alternative models of senescence. In this study, P-elements bearing the white minigene were inserted at random into a common genetic background, generating lines of D. melanogaster with single, stable transposon inserts. A series of 48 single-P-element lines revealed statistically significant heterogeneity in both longevity and fecundity. Longevity and early fecundity were only weakly positively correlated (r = 0.286, P = 0.0398). Both the pooled sample and 30 of the individual lines exhibited a leveling of age-specific mortality at advanced ages, in opposition to the classical demographic models. To the extent that these mutational effects are representative of naturally-occurring mutations in heterogeneous populations, this result presents a problem for the evolutionary theory of senescence. Natural selection is inefficient at removing deleterious mutations that are expressed only at late ages, and selection may not differentiate between mutations whose effects on longevity are post-reproductive. A leveling of the mortality rate would also be seen if mutations whose expression is delayed until very late simply do not occur. A simulation of mutation-selection balance among the 48 P-element tagged lines shows that the mean longevity declines monotonically with increasing mutation rate, consistent with the mutation-accumulation model.

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Stress and metabolic regulation in Drosophila

Cited by 5 publications

References 31 publications

Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila

Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila

DROSOPHILA NUTRIGENOMICS CAN PROVIDE CLUES TO HUMAN GENE-NUTRIENT INTERACTIONS

Probing the evolution of senescence inDrosophila melanogaster withP-element tagging

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