Stress and cell cycle regulation during somatic embryogenesis plays a key role in oil palm callus development

Ribeiro, Daiane Gonzaga; Almeida, Raphael Ferreira; Fontes, Wagner; Castro, Mariana de Souza; Sousa, Marcelo Valle de; Ricart, Carlos André Ornelas; Cunha, Raimundo Nonato Vieira da; Lopes, Ricardo; Scherwinski‐Pereira, Jonny Everson; Mehta, Ângela

doi:10.1016/j.jprot.2018.08.015

Cited by 19 publications

(21 citation statements)

References 56 publications

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“…In embryogenic development and callus stress response, removal and refolding of unnecessary and misfolded polypeptides are vital for cell reprogramming [24]. In alkaligrass callus, we found that salt increased HSP 90 and TCP1, as well as salt-decreased HSP70 and Hsp70-Hsp90 organizing protein 1 (HOP).…”

Section: Protein Synthesis and Processing Are Necessary For Callusmentioning

confidence: 98%

“…Cytoskeletal dynamics were revealed from salt-altered abundance patterns of three actin species (two salt-induced and one salt-reduced). Actin is necessary for callus formation in Arabidopsis [41] and different oil palm species [24,42]. The depolymerization of the actin cytoskeleton was taken as an important strategy for stress adaptation, triggering the execution of programmed cell death in nonembryogenic callus from maize [21,43].…”

Section: Salinity Signal Transduction and Cytoskeleton Dynamics In Camentioning

confidence: 99%

“…The two dimensional electrophoresis (2DE) gel-based and isobaric tags for relative and absolute quantification (iTRAQ)/tandem mass tag (TMT)-based quantitative approaches have been applied to reveal the molecular changes during callus development, differentiation and somatic embryogenesis of diverse plant species, such as sugar cane (Saccharum spp.) [18,19], maize (Zea mays) [20][21][22], rice (Oryza sativa) [23], oil palm (Elaeis oleifera × Elaeis guineensis) [24], Valencia sweet orange (Citrus sinensis) [25], Cyclamen persicum [26], Vanilla planifolia [27,28], and lotus (Nelumbo nucifera Gaertn. spp.…”

Section: Introductionmentioning

confidence: 99%

“…These studies provided valuable results toward understanding the molecular regulatory roles of H + -pumps (i.e., P H + -ATPase, V H + -ATPase, and H + -PPase), sucrose and pyruvate accumulations, ROS homeostasis, protein ubiquitination, phytohormone and growth regulators (e.g., auxin, cytokinin, abscisic acid and polyamine putrescine) in embryogenic competence acquisition in callus. Importantly, some critical proteins identified in these studies are probably potential biomarkers for embryogenic competence acquisition, whose functions are need to be further evaluated [24].…”

Section: Introductionmentioning

confidence: 99%

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NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

Zhang

et al. 2019

Preprint

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Background: Salinity has obvious effects on plant growth and crop productivity. The salt-responsive mechanisms have been well studied in differentiated organs (e.g., leaves, roots, and stems), but not in unorganized cells such as callus. High-throughput quantitative proteomics approaches have been used to investigate callus development, somatic embryogenesis, organogenesis, and stress response in numbers of plant species. However, they have not been applied to callus from monocotyledonous halophyte alkaligrass ( Puccinellia tenuifora ). Results: The alkaligrass callus growth, viability, and membrane integrity were disturbed by 50 mM and 150 mM NaCl treatments. Callus cells accumulated the proline, soluble sugar, and glycine betaine for the maintenance of osmotic homeostasis. Importantly, the activities of diverse ROS scavenging enzymes (e.g., SOD, APX, POD, GPX, MDHAR, and GR) and antioxidants (e.g., ASA, DHA, and GSH) were all induced by salinity. The abundance pattern of 55 salt-responsive proteins indicated that salt signal transduction, cytoskeleton, ROS scavenging, energy supply, gene expression, protein synthesis and processing, as well as other basic metabolism processes were altered in callus to cope with stress. Conclusions: The undifferentiated callus exhibited unique salinity-responsive mechanisms for ROS scavenging and energy supply. Activation of the POD pathway and AsA-GSH cycle was universal in callus and differentiated organs, but salinity-induced SOD pathway and salinity-reduced CAT pathway in callus were different from those in leaves and roots. To cope with salinity, callus mainly relied on glycolysis, but not the TCA cycle, for energy supply.

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Section: Protein Synthesis and Processing Are Necessary For Callusmentioning

confidence: 98%

Section: Salinity Signal Transduction and Cytoskeleton Dynamics In Camentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

Zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Cytoskeletal dynamic changes were revealed from the salt-altered abundance patterns of three actin species (two salt-increased and one decreased). Actin is necessary for callus formation in Arabidopsis [43] and different oil palm species [24,44]. The depolymerization of the actin cytoskeleton was taken as an important strategy for stress adaptation, triggering the execution of programmed cell death in non-embryogenic callus from maize [21,45].…”

Section: Salt Stress Signaling and Cytoskeleton Dynamics In Callusmentioning

confidence: 99%

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

Zhang

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: Salinity has obvious effects on plant growth and crop productivity. The salinity-responsive mechanisms have been well-studied in differentiated organs (e.g., leaves, roots and stems), but not in unorganized cells such as callus. High-throughput quantitative proteomics approaches have been used to investigate callus development, somatic embryogenesis, organogenesis, and stress response in numbers of plant species. However, they have not been applied to callus from monocotyledonous halophyte alkaligrass (Puccinellia tenuifora). Results: The alkaligrass callus growth, viability and membrane integrity were perturbed by 50 mM and 150 mM NaCl treatments. Callus cells accumulated the proline, soluble sugar and glycine betaine for the maintenance of osmotic homeostasis. Importantly, the activities of ROS scavenging enzymes (e.g., SOD, APX, POD, GPX, MDHAR and GR) and antioxidants (e.g., ASA, DHA and GSH) were induced by salinity. The abundance patterns of 55 salt-responsive proteins indicate that salt signal transduction, cytoskeleton, ROS scavenging, energy supply, gene expression, protein synthesis and processing, as well as other basic metabolic processes were altered in callus to cope with the stress. Conclusions: The undifferentiated callus exhibited unique salinity-responsive mechanisms for ROS scavenging and energy supply. Activation of the POD pathway and AsA-GSH cycle was universal in callus and differentiated organs, but salinity-induced SOD pathway and salinity-reduced CAT pathway in callus were different from those in leaves and roots. To cope with salinity, callus mainly relied on glycolysis, but not the TCA cycle, for energy supply. Keywords: Salinity response, ROS scavenging, Energy supply, Osmotic homeostasis, Callus, Halophyte alkaligrass, Proteomics

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Leaf transcriptomic signatures for somatic embryogenesis potential of Elaeis guineensis

et al. 2021

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Stress and cell cycle regulation during somatic embryogenesis plays a key role in oil palm callus development

Cited by 19 publications

References 56 publications

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis

Leaf transcriptomic signatures for somatic embryogenesis potential of Elaeis guineensis

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