Streptozotocin-induced diabetes modulates presynaptic and postsynaptic function in the rat ileum

Talubmook, Chusri; Forrest, Abigail S.; Parsons, Mike

doi:10.1016/s0014-2999(03)01722-9

Cited by 14 publications

(12 citation statements)

References 18 publications

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“…Diabetes was considered to be established by a blood glucose level of 200 mg dL -1 (Talubmook et al 2003). The STZ injection generally rendered rats diabetic within 1 week, as judged by the onset of polydipsia and polyuria, whereas the citrate buffer had no effect.…”

Section: Resultsmentioning

confidence: 99%

The short-circuit current of the ileum, but not the colon, is altered in the streptozotocin diabetic rat

Forrest

Makwana

Parsons

2006

Can. J. Physiol. Pharmacol.

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Ion transport in control and streptozotocin-diabetic rat colon and ileum was studied using the Ussing chamber technique. No differences were observed between control and diabetic colonic mucosal short-circuit current under either basal or carbachol (100 nmol/L-1 micromol/L)-stimulated or prostaglandin E2 (100 nmol/L-1 micromol/L)-stimulated conditions. Similarly to colonic tissues, no differences in the short circuit current in either carbachol-stimulated or prostaglandin E2-stimulated tissues were observed between control and diabetic ileal mucosa. The basal diabetic ileal short circuit current (99.58 +/- 22.67 microA) was significantly greater than that of control ileal tissues (29.67 +/- 4.45 microA). This difference was abolished by the sodium-glucose-cotransporter inhibitor, phloridzin (50 micromol/L) (118.00 +/- 28.09 microA vs. 25.60 +/- 4.59 microA) and was also prevented by the replacement of glucose with mannitol in the buffer bathing the apical side of the tissue (control: 17.05 +/- 5.85 microA vs. 17.90 +/- 3.10 microA). Acetazolamide (450 micromol/L; a carbonic anhydrase inhibitor), amiloride, and bumetanide (100 micromol/L each; Na+-channel blockers), piroxicam (50 micromol/L; a COX1 cyclooxygenase inhibitor), and ouabain (1 mmol/L; a K+ transport inhibitor) had no effect on the basal short circuit current of either control or diabetic ileal tissues. This indicated that the alteration in the basal short circuit current of diabetic ileal tissues was due to a change in cellular glucose transport, whereas the evoked changes in short circuit current were unaffected by the diabetic state.

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Section: Resultsmentioning

confidence: 99%

The short-circuit current of the ileum, but not the colon, is altered in the streptozotocin diabetic rat

Forrest

Makwana

Parsons

2006

Can. J. Physiol. Pharmacol.

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“…The rats were housed two per cage (one control, one STZ treated) and provided with 2% sucrose water for the first 48 h after injection to prevent hypoglycemia. Diabetes was verified by a blood glucose level of Ն200 mg/dl (42), measured using an Accu-Chek active blood glucose testing kit (Roche Diagnostics) on blood taken from the tail vein. In addition, further evidence of the successful inducement of diabetes in the STZ-injected rats included weight loss, polydipsia, and polyuria.…”

Section: Methodsmentioning

confidence: 99%

“…Neuropathy involving parasympathetic and sympathetic nerves and nonadrenergic, noncholinergic nerves have been reported (2,21,45,46), as well as alterations in the responsiveness to agonists such as carbachol (3), prostaglandin F 2␣ (42), and electrical field stimulation (29). Since spontaneous contractile activity in the gut is known to be affected by nitrergic nerves (11a, 31a), and since changes in nitrergic nerves have been reported in a model of spontaneous diabetes (47), we investigated changes in nNOS immunopositivity as possibly related to increased motor activity.…”

mentioning

confidence: 99%

Increase in stretch-induced rhythmic motor activity in the diabetic rat colon is associated with loss of ICC of the submuscular plexus

Forrest¹,

Huizinga²,

Wang³

et al. 2008

American Journal of Physiology-Gastrointestinal and Liver Physi

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Diabetes affects many aspects of gastrointestinal motility, in part due to changes in interstitial cells of Cajal (ICC). The effect of diabetes on the colon, however, is not well characterized, and the aim of the present study was to investigate possible relationships between altered colonic motility as a consequence of streptozotocin-induced diabetes and injury to ICC. Physiological, immunohistochemical, and ultrastructural techniques were employed. The motor pattern of the rat colon was dominated by rhythmic high-amplitude, low-frequency contractions that were primarily myogenic in origin. These rhythmic contractions were induced by stretch associated with increased tension; the amplitude of the superimposed rhythmic contractions increased with increasing applied tension. In diabetic rats, the stretch-induced rhythmic contractile activity remained robust and of similar frequency but was significantly higher in amplitude compared with that in control rats. At 700 mg of applied tension, the force of contraction in circular colonic muscle strips of the diabetic rats was 370% of control values. This robust presence of low-frequency contractions is consistent with the unaffected pacemaker, the ICC associated with Auerbach's plexus, and the increased amplitude correlates with loss of and injury to ICC of the submuscular plexus and intramuscular ICC. Loss of inhibitory nitrergic nerves does not appear to be a factor based on unaltered nNOS immunoreactivity.

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“…As expected, db/db mice had statistically higher blood glucose levels (563 mg/dl) than both the ob/ob mice and the control mice (P < 0.0001). While the ob/ob mice had slightly elevated blood glucose levels (148 mg/ dl) when compared to the controls (118 mg/dl), they were not considered to be diabetic as their blood glucose was < 200 mg/dl (Talubmook et al, 2003).…”

Section: Biosmentioning

confidence: 99%

Modeling Leptin Receptor Insensitivity by Comparing How Leptin or Leptin Receptor Mutations in Mice Affect Body Weight, Basal Metabolism, Body Temperature and Feeding Behaviors

Patton

DeGolier

2010

BIOS

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Streptozotocin-induced diabetes modulates presynaptic and postsynaptic function in the rat ileum

Cited by 14 publications

References 18 publications

The short-circuit current of the ileum, but not the colon, is altered in the streptozotocin diabetic rat

The short-circuit current of the ileum, but not the colon, is altered in the streptozotocin diabetic rat

Increase in stretch-induced rhythmic motor activity in the diabetic rat colon is associated with loss of ICC of the submuscular plexus

Modeling Leptin Receptor Insensitivity by Comparing How Leptin or Leptin Receptor Mutations in Mice Affect Body Weight, Basal Metabolism, Body Temperature and Feeding Behaviors

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