Streptozotocin diabetes in pregnant and nonpregnant rats

Golob, E; Rishi, Surendra; Becker, Kenneth L.; Moore, Charles F.

doi:10.1016/0026-0495(70)90024-7

Cited by 24 publications

(11 citation statements)

References 11 publications

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“…There are reports of some of the effects of streptozotocin treatment on blood glucose and other metabolic parameters in rats under various conditions and at different intervals after injection with streptozotocin (5,9,14,17). However, there has been no study to investigate when the metabolic changes of streptozotocin diabetes become established in normal, fed rats and over what period they may be sustained.…”

Section: Introductionmentioning

confidence: 99%

Time-Course of Changes in Blood Glucose and Ketone Bodies, Plasma Lipids and Liver Fatty Acid Composition in Streptozotocin-Diabetic Male Rats

Topping¹,

Targ²

1975

Horm Res

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Male rats were given streptozotocin (100 mg/kg) by intraperitoneal injection. Groups of control and streptozotocin-treated animals were sacrificed at daily intervals for 4 days after injection. Over this period, treated rats lost weight continuously while control animals progressively gained weight. Within 24 h of treatment blood glucose and plasma free fatty acids were raised to levels which were sustained for the remainder of the experiment. After 48 h blood ketone bodies, plasma cholesterol and triglycerides were maximally raised and liver glycogen and blood lactate similarly lowered. The percentage composition of major fatty acids in liver lipids was unchanged until 4 days after treatment when there were significant increases in the proportion of oleate and linoleate and reductions in stearate and arachidonate. The data confirm that streptozotocin induces a rapid and sustained diabetes. It is suggested that metabolic experiments, in streptozotocin-diabetic rats, may be performed 48 h after treatment.

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Section: Introductionmentioning

confidence: 99%

Time-Course of Changes in Blood Glucose and Ketone Bodies, Plasma Lipids and Liver Fatty Acid Composition in Streptozotocin-Diabetic Male Rats

Topping¹,

Targ²

1975

Horm Res

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“…Conversely, this neuroleptic significantly increased the duration of ultrasounds from the 4th up to the 16th day of age. Moreover, notable changes in the sound pressure levels as well as in the frequency of calls were produced by early postnatal treatment with H. (Golob et al, 1970;Pedersen, 1967). It is generally thought that reduction in the rate of blood flow to the uterus and its consequent effect on uterine and foetal growth may be involved in some of the complcations of diabetic pregnancy.…”

Section: Treatmentmentioning

confidence: 99%

“…Animals were pithed under halothane anaesthesia (Gillespie et Al, 1970;McGrath & Mackenzie, 1977) and were artificially ventilated with oxygen and given propranolol (1 mg/kg, i.v. at 20 min intervals).…”

Section: Pmentioning

confidence: 99%

Poster Communications

1985

British J Pharmacology

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It is well known that the anticonvulsant effect of antiepileptic drugs is reduced by repeated administration in both experimental animals and man (Frey and Kampmann, 1965; Masuda et al., 1979; Rawling et al., 1975). The purpose of this investigation was to verify if denzimol, an arylalkylimidazole anticonvulsant agent (Nardi et al., 1981; Graziani et al., 1983) induces tolerance after repeated treatment in mice. Phenobarbitone (PB) and carbamazepine (CB) were used as reference drugs.We have investigated the antielectroshock activity (MES) and the brain levels of denzimol, after acute and repeated treatment, to verify whether the development of denzimol tolerance involves mechanisms of adaptation of the central nervous system or an induction of hepatic microsomial enzymes.Three groups of 20 mice each were treated p.o. for 14 days with denzimol (30 mg/kg), PB (25 mg/kg) or CB (30 mg/kg). Three control groups of mice received the vehicle (0.5% methocel). 24 hours after the end of the prolonged treatment, the respective drugs and vehicle, at the same dose as above, were given to the treated and control groups of animals. The anticonvulsant effect of each drug against MES was examined at 1,2,4,6,8 hrs after the last administration. The protection against the tonic extension of forelimb (TFP) and hindlimb (THP) induced by MES was the criterium used for the assessment of positive response.After MES, animals treated with denzimol were sacrificed and brains were removed for tissue drug levels evaluation (Abbiati et al. 1985).The present study shows that repeated administrations of PB, CB and denzimol resulted in a development of tolerance to their anticonvulsant action. Tolerance induced by denzimol against TFP and THP extension, resulted lower than that of PB and probably of CB. When denzimol was administered in a single dose to denzimol tolerant mice, its brain concentrations was markedly reduced if compared to non-tolerant mice. Moreover the disappearance of brain denzimol concentrations, after a single dose of drug, was alterated by repeated treatment of mice with denzimol. These findings seem to suggest that decreased anticonvulsant activity of denzimol, following repeated administrations, might be due to a development of pharmacokinetic tolerance.Abbiati G.A., Restelli G.V., Graziani G., Testa R. (1985) In previous experim-ents we have shown that prolactin (PRL) secretion in avian species is under a tonic inhibitory control of dopa;-iine (disticb et al.,1979). The present study was carried out in order to assess the role played b, cholinergic mechanisms in the control of PRL secretion in pigeons (Columba livia). In particular, we have assessed the effects of drugs enhancing by different mechanisms cholinergic transmiission, i.e. carbachol, muscarine and physostigmine, on morphology (studied by SEM5 and TEll) of crop-sac mlucosa and pituitary lactoWrophs.Carbachol, microinfused into the III cerebral ventricle (6 nrmiol) for 3 consecutive days, produced intense crop-sac stimulation with rmiilk-like secretion and...

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“…Diabetes was induced by injecting streptozotocin (45 mg/kg body weight) in 50 mM sodium citrate buffer, pH 4.5, into the tail vein of pregnant rats on day 5 of gestation [8]. Controls were injected with buffer alone.…”

Section: Diabetic Pregnant Ratsmentioning

confidence: 99%

“…However, the use of experimental pro tocols differing in important details, such as the strain of the rat, the dose of streptozoto-cin injected, gestational age when the drug is administered, the documentation of hyper glycemia throughout gestation, the timing of surgical delivery, etc., have made it difficult to compare the data derived from various laboratories. Induction of chemical diabetes by fixed amounts of streptozotocin (SO SO mg/kg body weight) to pregnant rats pro duces a wide range of persistent hypergly cemia varying between 150 and 400 mg/dl of blood [4,8,11]. In the rat, it is well docu mented that persistent severe maternal hy perglycemia (> 250 mg/dl) significantly re duces the body weight of the offspring at birth [4,11,12] while mild maternal hyper glycemia (<200 mg/dl) significantly in creases the body weight of fetuses [4,11,12], Although a mild hyperglycemic condition in the pregnant rat can be induced by injecting low doses of streptozotocin (38 mg/kg body weight) [3], the number of experimental ani mals obtained by this means is unpredicta ble, with a considerable waste of pregnant animals because of a lack of induction of hyperglycemia or the reversal of a hypergly cemic state to a normoglycemic state later in gestation.…”

Section: Introductionmentioning

confidence: 99%

Effect of Glucose and Insulin Administration on Hepatic Adenylate Energy Charge and the Cytosolic Redox State in the Neonates of Normal and Insulin-Treated Diabetic Rats

Cuezva

Patel

1985

Neonatology

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At birth and during the first 3 postnatal hours, the concentrations of hepatic ATP and the adenylate energy charge were significantly higher in neonates of insulin-treated diabetic rats compared to age-matched neonates of normal rats. However, a significant reduction in these parameters was observed after 3 postnatal hours in neonates of insulin-treated diabetic rats compared to normal rats. Administration of glucose to newborns of insulin-treated diabetic rats at birth did not prevent the lowering of hepatic ATP concentrations and the adenylate energy charge in 6-hour-old neonates. In contrast, the administration of glucose or insulin to the newborns of normal rats at birth caused a reduction in the concentration of hepatic ATP and the adenylate energy charge only at 1 h after birth. The postnatal increase in the hepatic cytosolic redox state (NAD⁺/NADH) in the neonates of insulin-treated diabetic rats was similar to that observed in the neonates of normal rats during the first 3 postnatal hours; however, a significant reduction in this parameter was observed only in the neonates of insulin-treated diabetic rats after the third postnatal hour. Administration of glucose to the neonates of insulin-treated diabetic rats did not prevent the decline in the cytosolic redox state in these neonates at the sixth postnatal hour.

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Streptozotocin diabetes in pregnant and nonpregnant rats

Cited by 24 publications

References 11 publications

Time-Course of Changes in Blood Glucose and Ketone Bodies, Plasma Lipids and Liver Fatty Acid Composition in Streptozotocin-Diabetic Male Rats

Time-Course of Changes in Blood Glucose and Ketone Bodies, Plasma Lipids and Liver Fatty Acid Composition in Streptozotocin-Diabetic Male Rats

Poster Communications

Effect of Glucose and Insulin Administration on Hepatic Adenylate Energy Charge and the Cytosolic Redox State in the Neonates of Normal and Insulin-Treated Diabetic Rats

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