Streptozotocin and Alloxan in Experimental Diabetes. Comparison of the Two Models in Rats.

Islas-Andrade, Sergio; Revilla-Monsalve, Cristina; Escobedo, Jorge; Polanco, Ana C.; Palomino, Miguel A.; Velasco, Alfredo Feria

doi:10.1267/ahc.33.201

Cited by 21 publications

(10 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In concordance with literature [ 24 ], high glucose levels in the blood and urine of the diabetes-induced (via STZ) pregnant rats predominated throughout the study. Two days after treatment with the drug, blood glucose levels in the DRs were ≥200 mg/dL ( Table 2 ), and glycosuria reached 1,000 mg/dL.…”

Section: Resultssupporting

confidence: 85%

See 1 more Smart Citation

Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat

García

Maldonado

Revilla-Monsalve

et al. 2015

Journal of Diabetes Research

View full text Add to dashboard Cite

We investigated whether maternal diabetes induced in rats using streptozotocin (STZ) on Day 5 of pregnancy affects the intrauterine developmental timeline. A total of 30 pregnant Sprague-Dawley diabetic rats (DRs) and 20 control rats (CRs) were used to obtain 21-day fetuses (F21) and newborn (NB) pups. Gestational age, weight, and body size were recorded as were the maxillofacial morphometry and morphohistological characteristics of the limbs. In DRs, pregnancy continued for ∼1.7 days, and delivery occurred 23 days postcoitus (DPC). In this group, the number of pups was lower, and 13% had maxillofacial defects. F21 in the DR group had lower weights and were smaller; moreover, the morphological characteristics of the maxillofacial structures, derived from the neural crest, were discordant with their chronological gestational age, resembling 18- to 19-day-old fetuses. These deficiencies were counterbalanced in NB pups. We conclude that hyperglycemia, which results from maternal diabetes and precedes embryo implantation, deregulates the intrauterine developmental timeline, restricts embryo-fetal growth, and primarily delays the remodeling and maturation of the structures derived from neural crest cells.

show abstract

Section: Resultssupporting

confidence: 85%

“…The diabetes-inducing method was based on a protocol described in previous studies [ 9 , 24 ]. Briefly, on the fifth day of pregnancy (preimplantation period) STZ was injected intraperitoneally (Sigma, St. Louis, MO, USA) in a single 50-mg/kg dose.…”

Section: Methodsmentioning

confidence: 99%

Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat

García

Maldonado

Revilla-Monsalve

et al. 2015

Journal of Diabetes Research

View full text Add to dashboard Cite

show abstract

“…7a, b). STZ-treated mice show increased serum fatty acid, triglyceride and cholesterol levels [31]. There is a concern that Western blot analysis of VEGF-C expression; d: Quantitative analyses of the expression of VEGF-C. N = 8 per group.…”

Section: Discussionmentioning

confidence: 99%

Advanced Interfere Treatment of Diabetic Cardiomyopathy Rats by aFGF-Loaded Heparin-Modified Microbubbles and UTMD Technique

Zhang

Shen³

et al. 2016

Cardiovasc Drugs Ther

View full text Add to dashboard Cite

This study aims to investigate the preclinical performance and mechanism of a novel strategy of aFGF-loaded heparin-modified microbubbles (aFGF-HMB) combined with ultrasound-targeted microbubble destruction (UTMD) technique for diabetic cardiomyopathy (DCM) prevention. Type 1 diabetic rats were induced by streptozotocin. Twelve weeks after intervention, indexes from transthoracic echocardiography and cardiac catheterization showed that the left ventricular function in the aFGF-HMB/UTMD group was significantly improved compared with diabetes control (DM). From Picrosirius Red staining and TUNEL staining, the aFGF-HMB/UTMD group showed significant difference from the other groups. The cardiac collagen volume fraction (CVF) and myocardial cell apoptosis index (AI) in aFGF-HMB/UTMD group decreased to 7.2 % and 7.11 % respectively, compared with the DM group (CVF = 24.5 % and AI =20.3 % respectively). The results of myocardial microvascular density (MCD) also proved the strongest inhibition of aFGF-HMB/UTMD group on DCM progress. CD31 staining of aFGF-HMB/UTMD group reached 22 n/hrp, much higher than that of DM group (9 n/hrp). These results confirmed that the abnormalities including left ventricular dysfunction, myocardial fibrosis, cardiomyocytes apoptosis and microvascular rarefaction could be suppressed by twice weekly aFGF treatments for 12 consecutive weeks (free aFGF or aFGF-HMB+/-UTMD), with the strongest improvements observed in the aFGF-HMB/UTMD group (P < 0.05 vs free aFGF or aFGF-HMB). Western blot analyses of heart tissue further revealed the highest aFGF, anti-apoptosis protein (Bcl-2), VEGF-C, pAkt, pFoxo-3a levels and strongest reduction in pro-apoptosis proteins (Bax) level in aFGF-HMB/UTMD group. Overall, aFGF-HMB combined with UTMD technique might be developed as an effective strategy to prevent DCM in future clinical therapy.

show abstract

“…Using this protocol, rodents develop hyperglycemia within 7 to 14 days after the first injection. STZ-treated mice show increased serum fatty acid, triglyceride and cholesterol levels, whereas insulin levels progressively decrease with the duration of diabetes (Islas-Andrade et al, 2000).…”

Section: Models Of Type 1 Diabetes the Streptozotocin (Stz) Modelmentioning

confidence: 97%

Rodent models of diabetic cardiomyopathy

Bugger

Abel

2009

Disease Models &Amp; Mechanisms

245

198

View full text Add to dashboard Cite

Diabetic cardiomyopathy increases the risk of heart failure in individuals with diabetes, independently of co-existing coronary artery disease and hypertension. The underlying mechanisms for this cardiac complication are incompletely understood. Research on rodent models of type 1 and type 2 diabetes, and the use of genetic engineering techniques in mice, have greatly advanced our understanding of the molecular mechanisms responsible for human diabetic cardiomyopathy. The adaptation of experimental techniques for the investigation of cardiac physiology in mice now allows comprehensive characterization of these models. The focus of the present review will be to discuss selected rodent models that have proven to be useful in studying the underlying mechanisms of human diabetic cardiomyopathy, and to provide an overview of the characteristics of these models for the growing number of investigators who seek to understand the pathology of diabetes-related heart disease.

show abstract

Streptozotocin and Alloxan in Experimental Diabetes. Comparison of the Two Models in Rats.

Cited by 21 publications

References 27 publications

Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat

Importance of Maternal Diabetes on the Chronological Deregulation of the Intrauterine Development: An Experimental Study in Rat

Advanced Interfere Treatment of Diabetic Cardiomyopathy Rats by aFGF-Loaded Heparin-Modified Microbubbles and UTMD Technique

Rodent models of diabetic cardiomyopathy

Contact Info

Product

Resources

About