Streptomycin-Induced Chlorophyll-less Races of Euglena

Provasoli, Luigi; Hutner, S. H.; Schatz, Albert

doi:10.3181/00379727-69-16691

Cited by 169 publications

(46 citation statements)

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“…Plating efficiencies (number of colonies divided by total number of organisms plated) showed that streptomycin treatment had no effect on viability under resting conditions in the light or the dark, indicating that the streptomycin inhibition of chloroplast development was not caused by a loss of cell viability. Similar results were obtained when dividing organisms were used (Provasoli et al 1948). When streptomycin was added at the time of exposure of dark-grown resting organisms to light, there was a rapid loss of green-colony forming ability followed by a slower loss (Fig.…”

Section: Grcwi-coloiiy Fortizing Abilit)' Of Notdividing Organisinssupporting

confidence: 69%

Events Surrounding the Early Development of Euglena Chloroplasts: Experiments with Streptomycin in Non-dividing Cells

Bovarnick

Chang

Schiff

et al. 1974

Journal of General Microbiology

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The concentration of streptomycin (Sm) which selectively inhibits light-induced chloroplast development in non-dividing Euglena is the same as that which induces the loss of green-colony forming ability in dividing organisms. This concentration of Sm has no effect on division or viability. Chlorophyll synthesis is insensitive to streptomycin for the first 1 2 h of development but is strongly inhibited after this time. Between 72 and 96 h after the beginning of chloroplast development, Sm-treated organisms contain 10 yo of the chlorophyll and 24 of the carotenoids of algae developing in the absence of the antibiotic. The chlorophyll-to-carotenoid ratio in treated organisms at 72 to 96 h is 0.9, the same as is found at 1 2 h for organisms developing in the absence of Sm. In the presence of streptomycin, Euglena never develops the ability to fix CO, photosynthetically, although CO, fixation after I 2 h of development in the absence of the antibiotic can be readily detected. At I 2 h of chloroplast development the following parameters are at comparable levels in Sm-treated and untreated organisms: the bound forms of chlorophyll, concentration of cytochrome 552, the activities of ribulose diphosphate carboxylase, NADP-triose phosphate dehydrogenase, the enzymes converting ribose-yphosphate to ribulose diphosphate, and photosystem I1 activity measured as dye reduction.

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Section: Grcwi-coloiiy Fortizing Abilit)' Of Notdividing Organisinssupporting

confidence: 69%

Events Surrounding the Early Development of Euglena Chloroplasts: Experiments with Streptomycin in Non-dividing Cells

Bovarnick

Chang

Schiff

et al. 1974

Journal of General Microbiology

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“…Streptomycin binds to the 30S ribosome subunit and inhibits protein synthesis by interfering with the binding of the aminoacyl-tRNA to the ribosome and by stimulating miscoding (29); streptomycin inhibits chloroplast development (32) and has little or no effect on cytoplasmic protein synthesis of plant cells (25 The data obtained with apple skin and strawberry leaves (6, 8. 10) seem to indicate that photosynthesis may contribute photosynthetic products to light-dependent anthocyanin synthesis of green tissues with a functional photosynthetic apparatus.…”

Section: Resultsmentioning

confidence: 99%

Photocontrol of Anthocyanin Synthesis

Mancinelli¹,

Yang²,

Lindquist³

et al. 1975

Plant Physiol.

202

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“…This seems to be true also for the osmotrophic genera. A case in point is the placement of the respective Astasia representatives in different clades, thus rejecting the idea of Pringsheim & Hovasse (1948), Pringsheim & Pringsheim (1952), Proviasoli et al (1948), continued by Mego (1968) and Schiff & Eppstein (1968), that the genus Astasia as a whole evolved from an Euglena-like ancestor. The colourless genus Astasia seems to be polyphyletic, which confirms the earlier assumptions by Christen (1963) based on light microscopical investigations.…”

Section: Figmentioning

confidence: 96%

Phylogenetic analysis of phagotrophic, photomorphic and osmotrophic euglenoids by using the nuclear 18S rDNA sequence.

Müllner

Angeler

Samuel

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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Phylogenetic analyses of 35 strains including 25 previously published sequences and 10 which have been newly sequenced, representing two species of Euglena, five species of Phacus and three species of Astasia, were carried out using the SSU rDNA. Parsimony, distance and maximum-likelihood inferred phylogenies support (1) monophyly of the euglenoids, (2) kinetoplastids as the sister group, (3) the phagotrophic Petalomonas cantuscygni Cann et Pennick anchoring the base of the euglenoid lineage, (4) evolution of phototrophy within the euglenoids from a single event, (5) multiple origins of osmotrophic euglenoids and (6) polyphyly of the genera Euglena Ehrenberg and Phacus Dujardin. Analyses also indicate that Lepocinclis Perty, Trachelomonas Ehrenberg and Astasia Dujardin are polyphyletic. In addition, the results suggest that neither the Euglenales nor the Eutreptiales form a monophyletic lineage, thus questioning currently available classifications. Concerning the phagotrophic mode of nutrition, the data suggest that the feeding apparatus arose multiple times.

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Streptomycin-Induced Chlorophyll-less Races of Euglena

Cited by 169 publications

References 0 publications

Events Surrounding the Early Development of Euglena Chloroplasts: Experiments with Streptomycin in Non-dividing Cells

Events Surrounding the Early Development of Euglena Chloroplasts: Experiments with Streptomycin in Non-dividing Cells

Photocontrol of Anthocyanin Synthesis

Phylogenetic analysis of phagotrophic, photomorphic and osmotrophic euglenoids by using the nuclear 18S rDNA sequence.

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