Streptomycin and dihydrostreptomycin residues in bovine milk from the Brazilian retail market

Abstract

Pasteurised bovine milk from retail markets in the State of São Paulo, Brazil, was analysed for the presence of streptomycin (STP) and dihydrostreptomycin (DHSTP) residues. An ELISA kit was used for screening and a LC-APCI-MS/MS QToF method for confirmatory analysis. Both methods were intra-laboratory validated and found suitable for screening and confirmatory testing, respectively, for STP and DHSTP residues in pasteurised bovine milk at concentration levels below the maximum residue limit (MRL) established for these substances (200 µg kg(-1) expressed as the sum of the concentrations of STP and DHSTP). No residues of STP and DHSTP at detectable levels were found in the analysed samples (n = 299).

Anadolu Manda Sütlerinde Kanamisin Kalıntısının LC-MS/MS İle Belirlenmesi

“…Similarly, Unusan [25] performed an field monitoring study based on an ELISA method specific for aminoglycosides (streptomycin) in Turkey and reported that only one (1.7%) of 60 ultra-heat-treatment milk samples contained aminoglycosides. de Oliveira et al [26] analyzed a total of 299 pasteurized bovine milk samples collected from retail [13] LC-MS Bovine Milk 0.7 1.0 NA Goutalier et al [18] LC-MS/MS Bovine Milk 14 45 78-104 Bousova et al [19] LC-MS Bovine Milk 33 33 92 Saluti et al [20] LC-MS Bovine Milk <11.5 <18.5 81-91 Tao et al [21] LC-MS Bovine Milk 11 36 78-86 Yang et al [22] NA: Not available markets in Brazil by ELISA kit and an LC-APCI-MS/MS QToF method and reported that none of the samples contained streptomycin and dihydrostreptomycin residues. Also, 192 raw milk samples were analyzed in Hebei Province of China for the presence of twenty-eight veterinary drug residues including kanamycin tested by ultraperformance liquid chromatography with tandem mass spectrometry method and they reported none of the milk samples was contaminated with kanamycin [27] .…”

Survey of pyrethroid, macrocyclic lactone and antibacterial residues in bulk milk tank from Minas Gerais State, Brazil

“…Milk is one of the most consumed foods worldwide and its production has shown a continuous increase (Oliveira et al 2010). Brazil is one of the largest producers with with around 35.200 ton of milk per year.…”

Detection of antimicrobial and anthelmintic residues in bulk tank milk from four different mesoregions of Minas Gerais State - Brazil

“…Milk is one of the most consumed foods worldwide and its production has shown a continuous increase (Oliveira et al, 2010). Brazil is one of the largest producers and consumers of cow milk in the world, with about 30.7 billion liters of milk produced in 2010.…”

“…Failure to observe stipulated withdrawal periods for these drugs has resulted in several reports of their residues in milk. However, few data are available in Brazil, mainly when considering the anthelmintic drugs (Lobato et al, 2006;Nero et al, 2007;Bando et al, 2009;Fonseca et al, 2009;Oliveira et al, 2010;Zanella et al, 2010).…”

“…Although fast screening methods (i.e. immunological or microbial inhibition assays) are commonly used to detect the presence of antimicrobial and anthelmintics in foods, more accurate chromatographic methods are required by the governmental regulatory agencies to identify and confirm the presence of these compounds (Oliveira et al, 2010). …”