Streptomycete spores entrapped in chitosan beads as a novel biocontrol tool against common scab of potato

Jobin, Guy; Couture, Geneviève; Brzeziński, Ryszard; Beaulieu, Carole

doi:10.1007/s00253-004-1843-8

Cited by 24 publications

(12 citation statements)

References 22 publications

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“…The shift induced by chitosan in the soil microbial community occurred between 13 and 67 days after chitosan application. This delay could be attributed to both the kinetics of chitosan degradation (Jobin et al, 2005) and the fact that chitosan was not uniformly distributed in soil. Disturbance of microflora would possibly have been detected sooner at proximity to the seed tubers than in the bulk soil.…”

Section: Effects Of Chitosan and Ef-76 On Bacterial Communitiesmentioning

confidence: 98%

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Effect of chitosan and a biocontrol streptomycete on field and potato tuber bacterial communities

et al. 2006

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Four treatments applied on potato seed tubers i.e. talc only, chitosan only, Streptomyces melanosporofaciens strain EF-76 in talc and S. melanosporofaciens strain EF-76 in chitosan were compared for their efficiency to reduce common scab incidence. As previously reported the joint application of the geldanamycin-producing S. melanosporofaciens strain EF-76 and chitosan was effective to control common scab of potato. Nevertheless, field application of S. melanosporofaciens EF-76 did not allow the selection of geldanamycin-resistant actinomycetes (GRA) in the bulk soil during the potato growing season. The number of GRA on harvested potato tubers was, however, significantly higher in treatments that contained chitosan than in other treatments suggesting that chitosan might promote the establishment of the antagonistic actinomycete on progeny tubers. Biolog EcoPlates were used to determine the metabolic profiles of the bacterial soil communities. A permutation MANOVA analysis detected significant differences within the metabolic profiles of the bacterial communities at the potato flowering period but not at the beginning of the season or a week before harvest. A combination of S. melanosporofaciens EF-76 and chitosan thus represents a promising tool against common scab, with low short-term impact on soil bacterial communities.

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Section: Effects Of Chitosan and Ef-76 On Bacterial Communitiesmentioning

confidence: 98%

“…Enumeration of GRA was carried out as previously described (Jobin et al, 2005). Briefly, bacterial pellets recovered from soil samples and from potato tubers were resuspended in 1 ml NaCl solution (0.85%).…”

Section: Enumeration Of Gra In Soil and On Potato Tubersmentioning

confidence: 99%

Effect of chitosan and a biocontrol streptomycete on field and potato tuber bacterial communities

et al. 2006

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“…Indeed, chitin decomposing actinobacteria closely adhere to AMF spore walls (Selvakumar et al 2016) and the release of short chitin oligomers by chitinases leads to the activation of a signalling pathway involved in the first steps of AMF root colonization (Genre et al 2013). As an interesting way to introduce a chitinolytic actinobacterium in a plant production system, Jobin et al (2005) proposed the encapsulation of the microorganisms into chitosan beads. After their introduction in the environment, the beads could serve as carbon and nitrogen sources for the chitinolytic actinobacterium thus helping its implementation in the plant environment while the chitosan bead degradation would lead to the liberation of chitooligosaccharides that could act as elicitors of plant immunity responses (Jobin et al 2005).…”

Section: Chitinolytic Properties Of Actinobacteriamentioning

confidence: 99%

Chitinolytic functions in actinobacteria: ecology, enzymes, and evolution

Lacombe-Harvey

Brzeziński

Beaulieu

2018

Appl Microbiol Biotechnol

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Actinobacteria, a large group of Gram-positive bacteria, secrete a wide range of extracellular enzymes involved in the degradation of organic compounds and biopolymers including the ubiquitous aminopolysaccharides chitin and chitosan. While chitinolytic enzymes are distributed in all kingdoms of life, actinobacteria are recognized as particularly good decomposers of chitinous material and several members of this taxon carry impressive sets of genes dedicated to chitin and chitosan degradation. Degradation of these polymers in actinobacteria is dependent on endo- and exo-acting hydrolases as well as lytic polysaccharide monooxygenases. Actinobacterial chitinases and chitosanases belong to nine major families of glycosyl hydrolases that share no sequence similarity. In this paper, the distribution of chitinolytic actinobacteria within different ecosystems is examined and their chitinolytic machinery is described and compared to those of other chitinolytic organisms.Electronic supplementary materialThe online version of this article (10.1007/s00253-018-9149-4) contains supplementary material, which is available to authorized users.

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“…One could postulate that chitosan may contribute to disease suppression by eliciting plant defence mechanisms (Benhamou et al 1994) or by inhibiting the growth of the pathogen. This is unlikely the case since Jobin et al (2005) showed that chitosan beads were degraded within 1 wk after their addition to a soil substrate. The manure by-product used in this study was incubated in the presence of chitosan for over 1 wk before application on potato tubers.…”

Section: Discussionmentioning

confidence: 96%

Dehydrated pork manure by-product: effect of a chitosan amendment on bacterial community and common scab incidence

Roy

Lafontaine²,

Chabot³

et al. 2011

phyto

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Chitosan amendment modified the composition of a microbial community associated with dehydrated pork manure by-product. The amended product (biosolid PC) contained a lower number of anaerobic bacteria than the non-amended product (biosolid P). Chitosan also significantly reduced the fungal population. A 16S rRNA gene bank constructed from DNA extracted from the bacterial community associated with both P and PC biosolids revealed that bacterial ordersXanthomonodales,Pseudomonadales,Enterobacteriales,Burkholderiales,Actinomycetales,Bacillales,ClostridialesandLactobacillaleswere found in both biosolids. Bacteria from theStenotrophomonasgenus were abundant in both biosolids. However, the addition of chitosan appeared to induce changes in the population of some bacterial genera. For example, clones carrying a 16S rRNA gene corresponding to theBacillusgenus were doubled in biosolid PC. In field trials carried out to test their effect on common scab incidence, biosolids P and PC were applied as potato seed treatment. Biosolid P increased disease incidence by a factor of 1.33 and 2.85 in two independent experiments. However, when chitosan was added to the seed treatment, the stimulating effect of biosolid P on common scab was cancelled out.

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Streptomycete spores entrapped in chitosan beads as a novel biocontrol tool against common scab of potato

Cited by 24 publications

References 22 publications

Effect of chitosan and a biocontrol streptomycete on field and potato tuber bacterial communities

Effect of chitosan and a biocontrol streptomycete on field and potato tuber bacterial communities

Chitinolytic functions in actinobacteria: ecology, enzymes, and evolution

Dehydrated pork manure by-product: effect of a chitosan amendment on bacterial community and common scab incidence

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