Streptomyces sp. JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties

Stanković, Nebojša; Radulovic, Vanja; Petković, Miloš; Vučković, Ivan; Jadranin, Milka; Vasiljević, Branka; Nikodinovic-Runic, Jasmina

doi:10.1007/s00253-012-4237-3

Cited by 74 publications

(54 citation statements)

References 70 publications

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“…Compound E was compared to the well-known antioxidant butylatedhydroxyanisole, while compound F has shown antioxidant and UV protective properties in pigmented bacteria. 43 We found that the most effective compounds were compound A (anhydroexfoliamycin) and compound F (undecylprodigiosin) with a great antioxidant protection but achieved through different mechanisms. In this sense, anhydroexfoliamycin was not able to recover the mitochondrial function, but this compound showed a remarkable neuroprotection effect by eliminating ROS, maintaining the mitochondrial membrane potential and recovering CAT and GSH levels.…”

Section: ■ Results and Discussionmentioning

confidence: 92%

“…This is supported by previous reports of its effects in bacteria. This compound delayed H 2 O 2 lipid peroxidation and conferred the pigmented bacteria pronounced antioxidative and UV-protective properties, 43 that provide a survival advantage to the producing species. It is remarkable that compounds F and G (metacycloprodigiosin) are analogues included in the prodigiosin family.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Mitigation of ROS Insults by Streptomyces Secondary Metabolites in Primary Cortical Neurons

Leirós

Albrecht

Sánchez

et al. 2013

ACS Chem. Neurosci.

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Oxidative stress is a common point in neurodegenerative diseases, widely connected with mitochondrial dysfunction. In this study, we screened seven natural products from Streptomyces sources against hydrogen peroxide insult in primary cortical neurons, an oxidative stress in vitro model. We showed the ability of these compounds to inhibit neuronal cytotoxicity and to reduce ROS release after 12 h treatment. Among the tested compounds, the quinone anhydroexfoliamycin and the red pyrrole-type pigment undecylprodigiosin stand out. These two compounds displayed the most complete protection against oxidative stress with mitochondrial function improvement, ROS production inhibition, and increase of antioxidant enzyme levels, glutathione and catalase. Further investigations confirmed that anhydroexfoliamycin acts over the Nrf2-ARE pathway, as a Nrf2 nuclear translocation inductor, and is able to strongly inhibit the effect of the mitochondrial uncoupler FCCP over cytosolic Ca 2+ , pointing to mitochondria as a cellular target for this molecule. In addition, both compounds were able to reduce caspase-3 activity induced by the apoptotic enhancer staurosporine, but undecylprodigiosin failed to inhibit FCCP effects and it did not act over the Nrf2 pathway as was the case for anhydroexfoliamycin. These results show that Streptomyces metabolites could be useful for the development of new drugs for prevention of neurodegenerative disorders such as Parkinson's and Alzheimer's diseases and cerebral ischemia.

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Section: ■ Results and Discussionmentioning

confidence: 92%

Section: ■ Results and Discussionmentioning

confidence: 99%

Mitigation of ROS Insults by Streptomyces Secondary Metabolites in Primary Cortical Neurons

Leirós

Albrecht

Sánchez

et al. 2013

ACS Chem. Neurosci.

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“…The tumoricidal action of UPP alone and UPP conjugated to gold nanoparticles was observed in melanoma, lung, breast, and colon cancer cells, enabling the platform for further development of efficient anticancer drugs suitable for clinical application (22). On the other side, it has been shown that UPP provides protection against oxidative stress by delaying the lipid peroxidation process (24) and displaying the antioxidant properties and mitochondrial function improvement (25). Similar findings were obtained in our previous investigation showing that treatment of human lymphocytes with low UPP concentrations (0.1 µg mL -1 ) significantly reduced DNA damages and maintained the redox homeostasis of cells, indicating its cytoprotective nature.…”

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confidence: 92%

“…JS520 using mannitol-soy-yeast medium supplemented with methyloleate (0.2 %, v/v) for six days at 30 °C and recovered and purified by column chromatography. The quality of the purified UP was analysed by liquid chromatography coupled to mass spectroscopy (LC-MS) as described previously (24).…”

Section: Undecylprodigiosin Pigment (Upp) Preparationmentioning

confidence: 99%

The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro

Petrović

Vasic

Mitrović

et al. 2017

Archives of Industrial Hygiene and Toxicology

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Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in γ-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to γ-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.

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“…Some microorganisms that are capable for producing high efficiency pigments are as follow: Monascus, Serratia, Paecilomyces, Cordyceps and Streptomyces (60). It should be noted that Riboflavin, Beta-Carotene and Phycocyanin have high performances in traditional fermented food industry (63).…”

Section: Food Industrymentioning

confidence: 99%

The eminence status of bacterial pigments under different aspects

Sabbagh

Namvar

2017

Microbiol Med

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Several bacteria such as Flavobacterium, Serratias, Chromobacterium and Streptomyces that produce different pigments are playing a significant role in various fields of sciences. Alternatively, current knowledge about bacterial pigments is limited to medical bacteriology, for instance their importance in virulence factors and protective features, however recently the investigators have revealed the supplementary consequence of pigments in food, textile and pharmaceutical aspects.

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Streptomyces sp. JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties

Cited by 74 publications

References 70 publications

Mitigation of ROS Insults by Streptomyces Secondary Metabolites in Primary Cortical Neurons

Mitigation of ROS Insults by Streptomyces Secondary Metabolites in Primary Cortical Neurons

The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro

The eminence status of bacterial pigments under different aspects

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