Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity

Sandoval-Powers, Megan; Králová, Stanislava; Nguyen, Giang-Son; Fawwal, Dorelle V.; Degnes, Kristin F.; Lewin, Anna; Klinkenberg, Geir; Wentzel, Alexander; Liles, Mark R.

doi:10.1016/j.syapm.2021.126244

Cited by 15 publications

(5 citation statements)

References 84 publications

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“…For LN549, marine sponge-derived Streptomyces poriferorum P01-B04T, isolated from Geodia barretti from the Trondheim fjord of Norway, was the only closely related type strain in the TYGS database. The latter strain has been shown to have antimicrobial activity against Gram-positive bacteria ( Sandoval-Powers et al, 2021 ). The closest type strain related to Streptomyces sp.…”

Section: Resultsmentioning

confidence: 99%

Genomes and secondary metabolomes of Streptomyces spp. isolated from Leontopodium nivale ssp. alpinum

Malfent,

Zehl,

Kirkegaard

et al. 2024

Front. Microbiol.

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Bacterial endophytes dwelling in medicinal plants represent an as yet underexplored source of bioactive natural products with the potential to be developed into drugs against various human diseases. For the first time, several Streptomyces spp. were isolated from the rare and endangered traditional medicinal plant Leontopodium nivale ssp. alpinum, also known as Edelweiss. In the search for novel natural products, nine endophytic Streptomyces spp. from Edelweiss were investigated via genome sequencing and analysis, followed by fermentation in different media and investigation of secondary metabolomes. A total of 214 secondary metabolite biosynthetic gene clusters (BGCs), of which 35 are presumably unique, were identified by the bioinformatics tool antiSMASH in the genomes of these isolates. LC-MS analyses of the secondary metabolomes of these isolates revealed their potential to produce both known and presumably novel secondary metabolites, whereby most of the identified molecules could be linked to their cognate BGCs. This work sets the stage for further investigation of endophytic streptomycetes from Edelweiss aimed at the discovery and characterization of novel bioactive natural products.

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Section: Resultsmentioning

confidence: 99%

Genomes and secondary metabolomes of Streptomyces spp. isolated from Leontopodium nivale ssp. alpinum

Malfent,

Zehl,

Kirkegaard

et al. 2024

Front. Microbiol.

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“…Members of the genus Streptomyces are part of the family Streptomycetaceae . They constitute the largest genus in the domain of Bacteria [1]. This genus comprises aerobic bacteria with a Gram-positive, filamentous structures.…”

Section: Introductionmentioning

confidence: 99%

Streptomyces pyxinae sp. nov. and Streptomyces pyxinicus sp. nov. isolated from lichen Pyxine cocoes (Sw.) Nyl.

Somphong,

Polyiam,

Suriyachadkun

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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Two novel actinobacteria, designated as LP05-1T and LP11T, were isolated from the lichen Pyxine cocoes (Sw.) Nyl. collected in Bangkok, Thailand. Genotypic and phenotypic analyses revealed that both strains represented members of the genus Streptomyces. The 16S rRNA gene of LP05-1T showed the highest similarity to the genome of Streptomyces gelaticus (98.41 %), while the 16S rRNA gene of LP11T was most similar to that of Streptomyces cinerochromogenes (98.93 %). The major menaquinones in LP05-1T were MK-9(H8), MK-9(H6), MK-9(H4) and MK-9(H2), and in LP11T, they were MK-9(H8) and MK-9(H6). Both strains exhibited the major fatty acids iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0, with LP05-1T also possessing iso-C17 : 0. The polar lipids of LP05-1T included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified lipid, while those of LP11T consisted of phosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified aminolipid and an unidentified glycolipid. The digital DNA–DNA hybridisation (dDDH) and average nucleotide identity (ANI) values indicated that both strains are distinct from each other with values below 70 and 95 %, respectively. dDDH, ANI by blast (ANIb) and ANI by MUMmer (ANIm) values between LP05-1T and its closely related type strains were 26.07–26.80 %, 81.24–82.01 % and 86.82–86.96 %, respectively, while those for LP11T and its closely related type strains were 30.70–31.70 %, 84.09–85.31 % and 88.02–88.39 %, respectively. The results of the taxonomic investigation, including dDDH and ANI values, indicate that LP05-1T and LP11T are novel type strains of two novel species within the genus Streptomyces. The names proposed are Streptomyces pyxinae sp. nov. for strain LP05-1T (=TBRC 15494T, =NBRC 115434T) and Streptomyces pyxinicus sp. nov. for strain LP11T (=TBRC 15493T, =NBRC 115421T).

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“…A number of strains of S. griseoluteus are known for their ability to synthesize polyketide metabolites with antibacterial and antitumor activity, in particular griseolutein, U-77863, and U-77864 [17,18]. Th e synthesis of metabolites with anti-MRSA activity by strains of S. laculatispora has been reported [19].…”

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confidence: 99%

Sequences Similar to the lan-Cluster (Streptomyces cyanogenus S136) Were Found in the Genomes of Other Streptomycetes

Polishchuk

Lukyanchuk

2023

Mikrobiol. Z.

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The aim of the work is to identify strains of streptomycetes in the genomes of which there are nucleotide sequences similar to the gene cluster determining the synthesis of landomycin A (lan-cluster) and establish the level of similarity of their primary structures and organizations. Methods. Information on the sequences of the lan-cluster of Streptomyces cyanogenus S136 and chromosomal DNAs of S. cyanogenus S136, Streptomyces laculatispora NRRL B-24909, and Streptomyces griseoluteus JCM 4765 and their annotations are presented in the GenBank database on the NSBI server. A computerized analysis of the nucleotide sequences of streptomycetes was done using the program BLASTN from the server NSBI. Results. The localization of the lan-cluster in the terminal region of the S. cyanogenus S136 genome has been shown. The nucleotide sequences similar to the lan-cluster sequence of S. cyanogenus S136 were found in the genomes of two strains (S. laculatispora NRRL B-24909 and S. griseoluteus JCM 4765). Streptomycetes (S. cyanogenus S136, S. laculatispora NRRL B-24909, and S. griseoluteus JCM 4765) are not genetically related strains. Conclusions. There are newly found probable lan-clusters in the genomes of two streptomycetes strains (S. laculatispora NRRL B-24909 and S. griseoluteus JCM 4765). Landomycin clusters of three strains are organized according to the same scheme. The clusters of lan-genes are present in the genomes of genetically unrelated streptomycetes.

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Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity

Cited by 15 publications

References 84 publications

Genomes and secondary metabolomes of Streptomyces spp. isolated from Leontopodium nivale ssp. alpinum

Genomes and secondary metabolomes of Streptomyces spp. isolated from Leontopodium nivale ssp. alpinum

Streptomyces pyxinae sp. nov. and Streptomyces pyxinicus sp. nov. isolated from lichen Pyxine cocoes (Sw.) Nyl.

Sequences Similar to the lan-Cluster (Streptomyces cyanogenus S136) Were Found in the Genomes of Other Streptomycetes

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