Streptomyces populi sp. nov., a novel endophytic actinobacterium isolated from stem of Populus adenopoda Maxim

Wang, Zhikuan; Jiang, Binjin; Li, Xiaoguang; Gan, Longzhan; Long, Xiufeng; Zhang, Yuqin; Tian, Yongqiang

doi:10.1099/ijsem.0.002877

Cited by 11 publications

(5 citation statements)

References 34 publications

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“…The morphological, biochemical and physiological characteristics of SPRI-371 were evaluated to determine its cultural characteristics and carbon source utilization. Simultaneously, through a comparative analysis of the 16S rRNA sequence, it was found that SPRI-371 was closer to S. lanatus , but there were large differences in the morphological, biochemical and physiological characteristics [ 21 , 22 ]. Its location was shown in the neighbor-joining tree with a 99% approximation to S. aureus .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Aureonuclemycin Produced by Streptomyces aureus Strain SPRI-371

Wang

Feng²,

Li³

et al. 2022

Molecules

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Actinomycetes play a vital role as one of the most important natural resources for both pharmaceutical and agricultural applications. The actinomycete strain SPRI-371, isolated from soil collected in Jiangsu province, China, was classified as Streptomyces aureus based on its morphological, physiological, biochemical and molecular biological characteristics. Its bacterial activity metabolites were identified as aureonuclemycin (ANM), belonging to adenosine derivatives with the molecular formula C16H19N5O9 for ANM A and C10H13N5O3 for ANM B. Simultaneously, the industrial fermentation process of a mutated S. aureus strain SPRI-371 was optimized in a 20 m3 fermentation tank, featuring a rotation speed of 170 rpm, a pressure of 0.05 MPa, an inoculum age of 36–40 h and a dissolved oxygen level maintained at 1–30% within 40–80 h and at >60% in the later period, resulting in an ANM yield of >3700 mg/L. In the industrial separation of fermentation broth, the sulfuric acid solution was selected to adjust pH and 4# resin was used for adsorption. Then, it was resolved with 20% ethanol solution and concentrated in a vacuum (60–65 °C), with excellent results. Antibacterial experiments showed that ANM was less active or inactive against Xanthomonas oryzae pv. oryzae, Xanthomonas citri subsp. citri and Xanthomonas oryzae pv. oryzicola and most bacteria, yeast and fungi in vitro. However, in vivo experiments showed that ANM exhibited extremely significant protective and therapeutic activity against diseases caused by X. oryzae pv. oryzae and X. oryzae pv. oryzicola in rice and X. citri in oranges and lemons. In field trials, ANM A 150 gai/ha + ANM B 75 gai/ha exhibited excellent therapeutic activity against rice bacterial leaf blight, citrus canker and rice bacterial leaf streak. Furthermore, as the dosage and production cost of ANM are lower than those of commercial drugs, it has good application prospects.

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Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Aureonuclemycin Produced by Streptomyces aureus Strain SPRI-371

Wang

Feng²,

Li³

et al. 2022

Molecules

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“…Different parts of plants are sampled for the isolation of Streptomycetes, including tree bark [79], leaf litter [80], bulbil [81], roots [82][83][84], stem [85][86][87], fruits [88], seeds [89] and phylloplane [90] (Table 2). Mosses have also been recorded as a source of novel Streptomyces spp.…”

Section: Symbiontsmentioning

confidence: 99%

Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective

Donald

Pipite

Subramani

et al. 2022

Microbiology Research

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There is a real consensus that new antibiotics are urgently needed and are the best chance for combating antibiotic resistance. The phylum Actinobacteria is one of the main producers of new antibiotics, with a recent paradigm shift whereby rare actinomycetes have been increasingly targeted as a source of new secondary metabolites for the discovery of new antibiotics. However, this review shows that the genus Streptomyces is still the largest current producer of new and innovative secondary metabolites. Between January 2015 and December 2020, a significantly high number of novel Streptomyces spp. have been isolated from different environments, including extreme environments, symbionts, terrestrial soils, sediments and also from marine environments, mainly from marine invertebrates and marine sediments. This review highlights 135 new species of Streptomyces during this 6-year period with 108 new species of Streptomyces from the terrestrial environment and 27 new species from marine sources. A brief summary of the different pre-treatment methods used for the successful isolation of some of the new species of Streptomyces is also discussed, as well as the biological activities of the isolated secondary metabolites. A total of 279 new secondary metabolites have been recorded from 121 species of Streptomyces which exhibit diverse biological activity. The greatest number of new secondary metabolites originated from the terrestrial-sourced Streptomyces spp.

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“…+, positive; -, negative. ‡ Data from Lee et al [48]; † Data from Wang et al [49]; § Data from Sripreechasak et al [50]. Chemotaxonomic analyses revealed that strain NEAU-H2 T also exhibited the typical characteristics of the genus Streptomyces [47].…”

Section: Polyphasic Taxonomic Characterization Of Neau-h2 Tmentioning

confidence: 99%

Taxonomic Characterization, and Secondary Metabolite Analysis of Streptomyces triticiradicis sp. nov.: A Novel Actinomycete with Antifungal Activity

Han

et al. 2020

Microorganisms

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The rhizosphere, an important battleground between beneficial microbes and pathogens, is usually considered to be a good source for isolation of antagonistic microorganisms. In this study, a novel actinobacteria with broad-spectrum antifungal activity, designated strain NEAU-H2T, was isolated from the rhizosphere soil of wheat (Triticum aestivum L.). 16S rRNA gene sequence similarity studies showed that strain NEAU-H2T belonged to the genus Streptomyces, with high sequence similarities to Streptomyces rhizosphaerihabitans NBRC 109807T (98.8%), Streptomyces populi A249T (98.6%), and Streptomyces siamensis NBRC 108799T (98.6%). Phylogenetic analysis based on 16S rRNA, atpD, gyrB, recA, rpoB, and trpB gene sequences showed that the strain formed a stable clade with S. populi A249T. Morphological and chemotaxonomic characteristics of the strain coincided with members of the genus Streptomyces. A combination of DNA–DNA hybridization results and phenotypic properties indicated that the strain could be distinguished from the abovementioned strains. Thus, strain NEAU-H2T belongs to a novel species in the genus Streptomyces, for which the name Streptomyces triticiradicis sp. nov. is proposed. In addition, the metabolites isolated from cultures of strain NEAU-H2T were characterized by nuclear magnetic resonance (NMR) and mass spectrometry (MS) analyses. One new compound and three known congeners were isolated. Further, genome analysis revealed that the strain harbored diverse biosynthetic potential, and one cluster showing 63% similarity to natamycin biosynthetic gene cluster may contribute to the antifungal activity. The type strain is NEAU-H2T (= CCTCC AA 2018031T = DSM 109825T).

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Streptomyces populi sp. nov., a novel endophytic actinobacterium isolated from stem of Populus adenopoda Maxim

Cited by 11 publications

References 34 publications

Antibacterial Activity of Aureonuclemycin Produced by Streptomyces aureus Strain SPRI-371

Antibacterial Activity of Aureonuclemycin Produced by Streptomyces aureus Strain SPRI-371

Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective

Taxonomic Characterization, and Secondary Metabolite Analysis of Streptomyces triticiradicis sp. nov.: A Novel Actinomycete with Antifungal Activity

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