Streptomyces lonarensis sp. nov., isolated from Lonar Lake, a meteorite salt water lake in India

Abstract

A novel alkaliphilic actinomycete, strain NCL716(T), was isolated from a soil sample collected from the vicinity of Lonar Lake, an alkaline salt water meteorite lake in Buldhana district of Maharashtra State in India. The strain was characterised using a polyphasic taxonomic approach which confirmed that it belongs to the genus Streptomyces. Growth was observed over a pH range of 7-11 at 28 °C. The cell wall was found to contain LL-diaminopimelic acid and traces of meso-diaminopimelic acid. The major fatty acid components were identified as iso-C16:0 (46.8 %), C17:1 (12.4 %), anteiso-C15:0 (5.1 %) and anteiso-C17:1 (4.8 %). The major polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The major menaquinones were determined to be MK-9 (H6) (70.3 %), MK-9 (H4) (15.5 %) and MK-9 (H8) (7.2 %). The G+C content of the DNA of the type strain was determined to be 71.4 mol %. The 16S rRNA gene sequence has been deposited in GenBank with accession number FJ919811. Although the 16S rRNA gene sequence analysis revealed that strain NCL716(T) shares >99 % similarity with that of Streptomyces bohaiensis strain 11A07(T), DNA-DNA hybridization revealed only 33.2 ± 3.0 % relatedness between them. Moreover, these two strains can be readily distinguished by some distinct phenotypic characteristics. Hence, on the basis of phenotypic and genetic analyses, it is proposed that strain NCL716(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces lonarensis sp. nov., is proposed. The type strain is NCL 716(T) (=DSM 42084(T) = MTCC 11708(T) = KCTC 39684(T)).

Potential of Halophilic Bacteria for Extracellular Enzymes

“…In recent years presence of haloalkaliphilic methanogenic bacteria was revealed in this lake including a novel species, namely Methylophaga lonarensis (Antony et al, 2012). Sharma et al, (2016) also isolated a novel species from Lonar lake i.e. Streptomyces lonarsensis.…”

Streptomyces xanthii sp. nov. and Streptomyces roseirectus sp. nov. isolated from a Chinese medicinal plant

“…So far, the genus contains 671 species with validly published and correct names listed in the LPSN (https://lpsn.dsmz.de/genus/Streptomyces). Members of the genus Streptomyces grow in various environments, such as marine sponge [10], salt water lake [11], seawater [12], wetland soil [13] and plants [14], etc. In recent years, endophytic actinobacteria inhabiting tissues of plants have received significant attention due to their broad diversity and distinctive metabolic potential with medical, agricultural and industrial importance [15–18].…”

Draft Genome Sequence of Streptomyces sp. Strain ventii, Isolated from a Microbial Mat near Hydrothermal Vents within the Axial Seamount in the Pacific Ocean, and Resequencing of the Type Strains Streptomyces lonarensis NCL 716 and Streptomyces bohaiensis 11A07

“…Members of the genus Streptomyces are Gram-positive, spore-forming, filamentous bacteria that often synthesize desirable antimicrobials, cytotoxins, and other lead compounds ( 1 – 4 ). The type strains Streptomyces bohaiensis 11A07 and Streptomyces lonarensis NCL 716 produce antimicrobials and an α-amylase, respectively ( 5 – 7 ). Streptomyces sp.…”