Streptomyces liliifuscus sp. nov and an anti-ginger plague agent Streptomyces liliiviolaceus sp. nov, two novel species isolated from soil of Lilium lancifolium

Abstract

Two novel strains of actinobacteria, ZYC-3T and BH-SS-21T, were isolated from Hunan Province, PR China. The fermentation broth of BH-SS-21T inhibited the rapid spread of ginger blast, unlike that of ZYC-3T. The taxonomic characteristics of ZYC-3T and BH-SS-21T were defined using a polyphasic approach. The analysis of the full-length 16S rRNA gene sequence revealed that ZYC-3T and BH-SS-21T represented members of the genus Streptomyces . ZYC-3T had less than 98.7% sequence similarities to all species of the genus Streptomyces , while BH-SS-21T exhibited 99.97, 98.95, 98.83, 98.82, 98.75 and less than 98.7% sequence similarities to 'Streptomyces dioscori' A217, Streptomyces ederensis JCM 4958T, Streptomyces glomeroaurantiacus NBRC 15418T, Streptomyces aurantiacus NBRC 13017T, Streptomyces umbrinus JCM 4521T and other species with validly published names in the genus Streptomyces . However, the digital DNA–DNA relatedness and average nucleotide identity values between ZYC-3T, BH-SS-21T, and their closely related strains were significantly lower than the recommended threshold values. Also, phenotypic, chemotaxonomic and genetic features distinguished ZYC-3T and BH-SS-21T from their reference strains. On the basis of their genotypic and phenotypic characteristics, strains ZYC-3T and BH-SS-21T were classified as representing novel species of the genus Streptomyces under the names Streptomyces liliifuscus sp. nov. ZYC-3T (=CICC 25040T=JCM 34560T=MCCC 1K04978T) and Streptomyces liliiviolaceus sp. nov. BH-SS-21T (=MCCC 1K06236T=JCM 34767T), respectively.

Streptomyces beigongshangae sp. nov., isolated from baijiu fermented grains, could transform ginsenosides of Panax notoginseng

“…Similarly, phosphatidylethanolamine was detected in S. apricus SUN51 T . Additionally, the presence of phosphatidylethanolamine and phosphatidylinositol was also observed in S. liliiviolaceus BH-SS-21 T [35]. The predominant menaquinones of REN17 T were MK-9 (H 2 ), MK-9 (H 4 ), MK-9 (H 6 ) and MK-9 (H 8 ).…”

Discovery and biosynthetic assessment of 'Streptomyces ortus' sp. nov. isolated from a deep-sea sponge

“…pneumoniae [71] and S. liliiviolaceus BH-SS-21 T reporting activity against the Gram-negative plant pathogen Ralstonia solanacearum [70]. This suggests that the metabolomic profile of A15ISP2-DRY2 T is distinct from its close relatives and highlights the poorly understood relationship between the genome and metabolome of a strain [72, 73].…”

“…dioscori' A217 T ), despite these strains being isolated from drastically different environments: ' S. dioscori' from a yam plant and S. liliiviolaceus from soil [70, 71]. Interestingly, the related strains show distinct bioactivities, with 'S.…”

Discovery and biosynthetic assessment ofStreptomyces ortussp nov. isolated from a deep-sea sponge

“…These clusters represent priority targets for compound isolation or, if not produced under standard laboratory conditions, heterologous expression (43, 44). A15ISP2-DRY2 T shared the majority of the identified GCFs with its closest relatives ( S. liliiviolacens BH-SS-21 T and S. dioscori A217 T ’), despite these strains being isolated from drastically different environments: S. dioscori from a yam plant and S. liliiviolacens from soil (45, 46). Interestingly, the related strains show distinct bioactivities, with ‘S.…”

“…Interestingly, the related strains show distinct bioactivities, with ‘S. dioscori’ A217 having activity solely against Gram negative Klebsiella pneumoniae (46) and S. liliiviolacens BH-SS-21 T reporting activity against the Gram negative plant pathogen Ralstonia solanacearum (45). This suggests that the metabolomic profile of A15ISP2-DRY2 T is distinct from its close relatives and highlights the poorly understood relationship between the genome and metabolome of a strain (47, 48).…”