Streptomyces coryli sp. nov., isolated from hazelnut orchard soil

Abstract

A novel actinobacteria, isolate A7024T, was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 16S rRNA gene sequences indicated that isolate A7024T was positioned within the members of the genus Streptomyces with the highest sequence similarity (97.7 %) to Streptomyces cadmiisoli ZFG47T. The organism formed an extensively branched substrate and aerial hyphae which generated irregular rod-shaped spores with smooth-surfaces. The cell wall of strain A7024T contained ll-diaminopimelic. Glucose, mannose and ribose were detected as whole-cell sugars. Its polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids and three unidentified glycolipids. Major menaquinones were MK-9(H6) and MK-9(H4). The major cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. Strain A7024T had a genome size of 9.0 Mb with a genome G+C content of 71.5 mol%. The low level of 16S rRNA gene similarity, 19.3 ± 2.3% digital DNA–DNA hybridization and 76.94 % average nucleotide identity values, as well as some different phenotypic characteristics allowed the strain to be distinguished from the closely related type strains. Therefore, it is concluded that strain A7024T represents a novel species of the genus of Streptomyces , for which the name Streptomyces coryli sp. nov. is proposed. The type strain is A7024T (=DSM 42066T=KCTC 29102T=NRRL B-24888T).

Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective

“…In addition, the selective isolation of actinobacteria by suspending the sample in 1.5% phenol for 30 min is a chemical pretreatment used [138] that disrupts the cell wall of other common bacteria and fungi and improves the growth of Streptomyces [94]. Furthermore, treatment of samples by airdrying for a week [121,130,147] or two [129,136] is a commonly used pretreatment method (Table 5). Moreover, different carbon sources (cellulose, glucose, chitin, starch, dextrose, mannitol and proteose) and combined nitrogen-carbon sources (casein, yeast extract, malt extract, tryptone and peptone) have been added to the media for successful isolation of Streptomycetes [128,129,133,136] from soil sediments (see Table 4 and Supplementary Notes on Supplementary Table S1).…”

“…Furthermore, treatment of samples by airdrying for a week [121,130,147] or two [129,136] is a commonly used pretreatment method (Table 5). Moreover, different carbon sources (cellulose, glucose, chitin, starch, dextrose, mannitol and proteose) and combined nitrogen-carbon sources (casein, yeast extract, malt extract, tryptone and peptone) have been added to the media for successful isolation of Streptomycetes [128,129,133,136] from soil sediments (see Table 4 and Supplementary Notes on Supplementary Table S1). Highlighted data shows that soil and sediment are the main source of new Streptomyces spp.…”

“…supplemented with cycloheximide (50 µg mL −1 ), nalidixic acid (10 µg mL −1 ), nystatin (50 µg mL −1 ) and novobiocin (10 µg mL −1 ) 28 • C for 21 days. [129] Heating at 55 Wet heat (20 min, 60 • C) Crater lake sediments ISP 2 medium supplemented with 10 mg/L tetracycline, nystatin (50 µg/mL) and rifampicin (5 mg mL −1 ) 28 • C for 14 days [58] Heat treated at 120 Suspended in distilled water (2 mL) followed by an ultrasonic treatment (160 W) for 3 min + soil suspension was incubated at 28 • C and 250 r.p.m. on a rotary shaker for 30 min Rhizosphere soil of Urtica urens L.…”

“…(50 µg mL −1 ), nalidixic acid (10 µg mL −1 ), nystatin (50 µg mL −1 ) and novobiocin (10 µg mL −1 ) Turkey[129] Streptomyces paludis sp. nov.…”

Streptomyces musisoli sp. nov., an actinomycete isolated from soil

“…nov. [11], Streptomyces coryli sp. nov. [12], Streptomyces cupreus sp. nov. [13], Streptomyces montanus sp.…”