Streptomyces catbensis sp. nov., isolated from soil

Abstract

Strain VN07A0015 T was isolated from soil collected on Cat Ba Island, Vietnam. The taxonomic position of strain VN07A0015 T was near Streptomyces aomiensis M24DS4 T (98.5 % 16S rRNA gene sequence similarity) and Streptomyces scabrisporus NBRC 100760 T (95.6 %), and it clustered within them; however, this cluster was distant from the type strains of other species of the genus Streptomyces. The aerial mycelia of strain VN07A0015 T were greyish and formed imperfect spiral spore chains (retinaculiaperti type) with smooth-surfaced spores. The morphological features of strain VN07A0015 T were different from those of the type strains of S. aomiensis and S. scabrisporus. The chemotaxonomic characteristics of strain VN07A0015 T were typical for all members of the genus Streptomyces, which possessed LL-type diaminopimelic acid, menaquinone MK-9(H 6 , H 8 ) and the major fatty acids iso-C 16 : 0 and iso-C 15 : 0 . DNA-DNA relatedness between strain VN07A0015 T and S. aomiensis NBRC 106164 T was less than 30 %. In addition, some physiological and biochemical traits differed from those of S. aomiensis. Therefore, we propose that strain VN07A0015 T be classified in the genus Streptomyces as a representative of Streptomyces catbensis sp. nov. (type strain VN07A0015 T 5VTCC-A-1889 T 5NBRC 107860 T ).

Genome-Based Taxonomic Classification of the Phylum Actinobacteria

“…A highly supported clade composed of S. aomiensis (Nagai et al, 2011 ), S. catbensis (Sakiyama et al, 2014 ), and S. seranimatus (Wang et al, 2007c ) was found to branch before S. scabrisporus but after S. thermoautotrophicus in the CCT. The three species share biochemical, cultural, and morphological features, notably the formation of smooth spores in straight to flexuous or looped spore chains, properties that distinguished them from their nearest phylogenetic neighbor, S. scabrisporus , which produces rugose ornamented spores in spiral chains (Ping et al, 2004 ; Wang et al, 2007c ; Sakiyama et al, 2014 ). In turn, S. seranimatus can be distinguished from all other Streptomycetaceae given the presence of PC in its polar lipid profile (Supplementary Table 1 ).…”

Classification of ‘Streptomyces hyalinum’ Hamada and Yokoyama as Embleya hyalina sp. nov., the second species in the genus Embleya, and emendation of the genus Embleya

“…S1 (available in the online version of this article) whereas the major fatty acids were C 16 : 0 (22 %), iso -C 16 : 0 (18 %), anteiso -C 15 : 0 (14 %) and iso -C 15 : 1 (12 %). The presence of a high proportion of an unsaturated fatty acid is unusual in major members of the family Streptomycetaceae , but rather common in recently established genera such as Embleya and Yinghuangia [1, 4, 10, 11]. The in silico DNA G+C content of ‘ S. hyalinum ’ NBRC 13850 T was 71.7 %.…”

“…White to light grey aerial mycelium was observed on ISP 2-7 and glucose-asparagine agars. In contrast, substrate mycelium of E. scabrispora shows different colours such as pearl, and aerial mycelium was observed only on oatmeal agar (Table 2) [3,5,10]. Table 1 shows the morphological, cultural, cellchemical, physiological and biochemical characteristics of 'S.…”

Antimicrobial activity of actinomycetes and characterization of actinomycin-producing strain KRG-1 isolated from Karoo, South Africa

“…Isolate KRG-1 showed positive activity for nitrate reduction, alkaline phosphatase, β-galactosidase, α-glucosidase and gelatine hydrolysis. Majority of streptomycete species showed positive activity for the mentioned activities (Khan et al, 2010;Sakiyama et al, 2014).…”

“…To our knowledge, Streptomyces and Micromonospora species were reported capable of producing actinomycins (Kurosawa et al, 2006;Praveen, Tripathi, 2009). Antimycines were isolated for example from Streptomyces parvulus RSPSN2 (Keller et al, 2010), Streptomyces sindenensis (Praveen et al, 2008), Streptomyces avermitilis MS449 (Watkins et al, 1998), Streptomyces griseoruber (Shetty et al, 2014), Streptomyces nasri (Chen et al, 2012), Streptomyces chrysomallus (Sakiyama et al, 2014) and Streptomyces triostinicus (Praveen et al, 2009). There was a previous reports stating that also Streptomyces antibioticus produced actinomycin (El-Naggar, 1988; Singh et al, 2009) -however, not from South Africa and different species reported by different research groups may be the same species due to a lack of standard strain and not enough information regarding the characteristics of each strain (Shirling, Gottlieb, 1966).…”