Streptomyces atlanticus sp. nov., a novel actinomycete isolated from marine sponge Aplysina fulva (Pallas, 1766)

Abstract

The taxonomic position of a novel marine actinomycete isolated from a marine sponge, Aplysina fulva, which had been collected in the Archipelago of Saint Peter and Saint Paul (Equatorial Atlantic Ocean), was determined by using a polyphasic approach. The organism showed a combination of morphological and chemotaxonomic characteristics consistent with its classification in the genus Streptomyces and forms a distinct branch within the Streptomyces somaliensis 16S rRNA gene tree subclade. It is closely related to Streptomyces violascens ISP 5183 (97.27 % 16S rRNA gene sequence similarity) and Streptomyces hydrogenans NBRC 13475 (97.15 % 16S rRNA gene sequence similarity). The 16S rRNA gene similarities between the isolate and the remaining members of the subclade are lower than 96.77 %. The organism can be distinguished readily from other members of the S. violacens subclade using a combination of phenotypic properties. On the basis of these results, it is proposed that isolate 103 (=NRRL B-65309 = CMAA 1378) merits recognition as the type strain of a new Streptomyces species, namely Streptomyces atlanticus sp. nov.

Streptomyces: Still the Biggest Producer of New Natural Secondary Metabolites, a Current Perspective

“…from marine samples [182]. In addition to humic acid isolation agar [179,184], other isolation agars such as actinomycete isolation agar [181], inorganic salt-starch agar [183] and starch and casein [180] have also been used to isolate new Streptomycetes from the marine environment. In comparison, humic acid and starch are the only two sources of carbon supplemented reported for us for the isolation of marine invertebrate Streptomyces [179,181,182].…”

“…Other isolation media have been summarized in Table 6. Notably, a sample underwent chemical pretreatment using 3% NaCl [184]. Three percent NaCl has proven to be the optimum concentration that supports actinobacterial growth compared to concentrations lower or higher than 3% [185].…”

“…Humic acid vitamin (HV) agar amended with 3% NaCl, nystatin 100 µg mL −1 , cycloheximide (100 µ g mL −1 ) and nalidixic acid (50 µg mL −1 ) Brazil [184] Streptomyces hyaluromycini sp. nov.…”

Streptomyces cahuitamycinicus sp. nov., isolated from desert soil and reclassification of Streptomyces galilaeus as a later heterotypic synonym of Streptomyces bobili

“…This genus is responsible for a significant portion of all isolated and identified natural compounds such as antibiotics [10, 11]. For this reason, the genus Streptomyces has become the main focus of isolation and investigation studies of the novel species from different habitats such as deserts [12–15], bats [16], fruits [17], insects [18, 19], lake sediments [20, 21], manganese-contaminated soil [22], mangrove sediments [23, 24], mangrove soil [25], marine sponges [26, 27], marine sediments [28, 29], rhizospheres [30, 31], zinc and copper mine [32], and plant tissues [33, 34] and soil [35, 36]. In this study, it was aimed to isolate novel actinobacteria from soil samples collected from the Karakum Desert.…”

Actinobacteria from Antarctica as a source for anticancer discovery

“…Compound classes belonging to the peptides, polyketides, macrolides, quinolones and others represent these bioactivities [28][29][30][31][32] . Accordingly, the isolation and identification of actinomycetes have become a fruitful area of research in recent years that has subsequently led to the identification of novel Actinomycete species that should be exploited to unveil possible biosynthetic pathways and discover new bioactive natural products 12,[33][34][35] . Thus, the rhizosphere bacterial composition of Antarctic hairgrass (Fig.…”