Streptomyces artemisiae MCCB 248 isolated from Arctic fjord sediments has unique PKS and NRPS biosynthetic genes and produces potential new anticancer natural products

Dhaneesha, Mohandas; Naman, C. Benjamin; Krishnan, K. P.; Sinha, Rupesh Kumar; Puthumana, Jayesh; Joseph, V.; Singh, I. S. Bright; Gerwick, William H.; Puthiyedathu, Sajeevan Thavarool

doi:10.1007/s13205-017-0610-3

Cited by 16 publications

(10 citation statements)

References 30 publications

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“…The results were similar to the studies of Sharma et al ( 2016 ) and Passari et al ( 2015 ), and it was shown that actinomycetes possessing antifungal activity were positive for the presence of both of these two biosynthetic pathway genes in their genomes. The presence of genes encoding PKS-I and NRPS in strain CB-75 is indicative of the possibility that it can produce bioactive secondary metabolites belonging to these two classes of natural products, or a hybrid of both (Dhaneesha et al, 2017 ). Relatively low sequence similarity of the PKS-I gene sequences (84%) with those available in GenBank is indicative of the possibility that novel compounds are produced by Streptomyces sp.…”

Section: Discussionmentioning

confidence: 99%

Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

Chen

Zhou

et al. 2018

Front. Microbiol.

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An actinomycete strain, CB-75, was isolated from the soil of a diseased banana plantation in Hainan, China. Based on phenotypic and molecular characteristics, and 99.93% sequence similarity with Streptomyces spectabilis NBRC 13424 (AB184393), the strain was identified as Streptomyces sp. This strain exhibited broad-spectrum antifungal activity against 11 plant pathogenic fungi. Type I polyketide synthase (PKS-I) and non-ribosomal peptide synthetase (NRPS) were detected, which were indicative of the antifungal compounds that Streptomyces sp. CB-75 could produce. An ethyl acetate extract from the strain exhibited the lowest minimum inhibitory concentration (MIC) against Colletotrichum musae (ATCC 96167) (0.78 μg/ml) and yielded the highest antifungal activity against Colletotrichum gloeosporioides (ATCC 16330) (50.0 μg/ml). Also, spore germination was significantly inhibited by the crude extract. After treatment with the crude extract of Streptomyces sp. CB-75 at the concentration 2 × MIC, the pathogenic fungi showed deformation, shrinkage, collapse, and tortuosity when observed by scanning electron microscopy (SEM). By gas chromatography-mass spectrometry (GC-MS) of the crude extract, 18 chemical constituents were identified; (Z)-13-docosenamide was the major constituent. Pot experiments showed that the incidence of banana seedlings was reduced after using Streptomyces sp. CB-75 treatment. The disease index was 10.23, and the prevention and control effect was 83.12%. Furthermore, Streptomyces sp. CB-75 had a growth-promoting effect on banana plants. The chlorophyll content showed 88.24% improvement, the leaf area, root length, root diameter, plant height, and stem showed 88.24, 90.49, 136.17, 61.78, and 50.98% improvement, respectively, and the shoot fresh weight, root fresh weight, shoot dry weight, and root dry weight showed 82.38, 72.01, 195.33, and 113.33% improvement, respectively, compared with treatment of fermentation broth without Streptomyces sp. CB-75. Thus, Streptomyces sp. CB-75 is an important microbial resource as a biological control against plant pathogenic fungi and for promoting banana growth.

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Section: Discussionmentioning

confidence: 99%

Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

Chen

Zhou

et al. 2018

Front. Microbiol.

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“…For more than 40 years, small organic molecules (<3,000 Daltons) derived from microbes and plants have been used as chemotherapy agents for cancer (Kinghorn et al, 2009). The Streptomyces bacteria are the main source of medicinal compounds derived from microbes for nearly 80% of drugs, especially antibiotics (Weber et al, 2015) and anticancer agents (Dhaneesha et al, 2017;Nguyen et al, 2020). In our previous research, we discovered a marine bacterium, Streptomyces sp.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and molecular mechanism of marine-derived Streptomyces sp. GMY01 on human lung cancer cell line A549

Damayanti¹,

Nisa²,

Handayani³

et al. 2020

J Appl Pharm Sci

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Marine-derived Streptomyces sp. GMY01 is a biotechnologically potential bacterium which has anticancer activity. This research aimed to investigate the anticancer activity of GMY01 extract on human lung cancer cell line A549 and its mechanism using Western blot analysis and in silico molecular docking. Ethyl acetate extract was obtained from the supernatant of an 11-day fermented product and it was fractionated using flash chromatography. An in vitro assay of the selected fractions (F5, F6, F7, F8, and F14) on the A549 cell line showed moderate activity (IC 50 value of 18.41-231.6 µg/ml), whereas the IC 50 value of the crude extract (CE) was 34.26 µg/ml. Western blot analysis revealed the mode of action of GMY01 fractions F6 and F7 to be that of an autophagy induction mechanism, similar to that of the CE. Targeted liquid chromatography high-resolution mass spectrometry analysis of GMY01 extract detected eight compounds: herboxidiene (C 25 H 42 O 6 ), vazabitide A (C 12 H 21 N 3 O 4 ), albaflavenone (C 15 H 22 O), grincamycin (C 49 H 62 O 18 ), isorenieratene (C 40 H 48 ), geosmin (C 12 H 22 O), hopene (C 30 H 50 ), and saframycin A (C 60 H 68 N 10 O 14 ). A molecular docking study on target proteins of antiapoptotic proteins (BCL-2 and BCL-XL) and autophagy proteins mammalian target of rapamycin (mTOR-C1 and mTORC2) showed that these compounds had a high affinity. Among all the compounds, grincamycin (C 49 H 62 O 18 ) had the highest affinity on antiapoptotic proteins (−11.8 and −10.4) and on autophagy proteins (−12.1 and −11.5) within all binding domains. These results indicate that Streptomyces sp. GMY01 has a promising anticancer agent, grincamycin, for human lung cancer.

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“…Due to the rich microbial diversity and unique selective pressures experienced by the Arctic microbiome, we hypothesize that this extreme environment has potential for harboring novel antibacterial secondary metabolites. Previous bioprospecting studies conducted in polar cryohabitats have successfully identified microbial isolates expressing new natural products and encoding unknown secondary metabolite BGCs (Mitova et al, 2005; Tedesco et al, 2016; Dhaneesha et al, 2017). One such example includes the discovery of Streptomyces artemisiae MCCB 248 isolated from sediments in the Arctic fjord Kongsfjorden.…”

Section: Introductionmentioning

confidence: 99%

“…One such example includes the discovery of Streptomyces artemisiae MCCB 248 isolated from sediments in the Arctic fjord Kongsfjorden. This isolate was shown to produce secondary metabolites with anticancer properties and was found to encode unique polyketide synthetase (PKS) and non-ribosomal peptide (NRP) genes (Dhaneesha et al, 2017). Isolating secondary-metabolite-producing microbes from Arctic environments has the additional benefit of yielding strains with inhibitory activity at cold temperatures.…”

Section: Introductionmentioning

confidence: 99%

Culture-Dependent Bioprospecting of Bacterial Isolates From the Canadian High Arctic Displaying Antibacterial Activity

et al. 2019

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The goal of this study was to isolate, screen, and characterize Arctic microbial isolates from Expedition Fjord, Axel Heiberg Island, Nunavut, Canada capable of inhibiting the growth of foodborne and clinically relevant pathogens. Arctic bacteria were isolated from twelve different high Arctic habitats pertaining to active layer permafrost soil, saline spring sediments, lake sediments, and endoliths. This was achieved using (1) the cryo-iPlate, an innovative in situ cultivation device within active layer permafrost soil and (2) bulk plating of Arctic samples by undergraduate students that applied standard culturing methods. To mitigate the possibility of identifying isolates with already-known antibacterial activities, a cell-based dereplication platform was used. Ten out of the twelve Arctic habitats tested were found to yield cold-adapted isolates with antibacterial activity. Eight cold-adapted Arctic isolates were identified with the ability to inhibit the entire dereplication platform, suggesting the possibility of new mechanisms of action. Two promising isolates, initially cultured from perennial saline spring sediments and from active layer permafrost soil ( Paenibacillus sp. GHS.8.NWYW.5 and Pseudomonas sp. AALPS.10.MNAAK.13, respectively), displayed antibacterial activity against foodborne and clinically relevant pathogens. Paenibacillus sp. GHS.8.NWYW.5 was capable of inhibiting methicillin resistant and susceptible Staphylococcus aureus (MRSA and MSSA), Listeria monocytogenes , Salmonella enterica and Escherichia coli O157:H7. Pseudomonas sp. AALPS.10.MNAAK.13 was observed to have antagonistic activity against MRSA, MSSA, Acinetobacter baumanii , Enterococcus faecium , and Enterococcus faecalis . After whole genome sequencing and mining, the genome of Paenibacillus sp. GHS.8.NWYW.5 was found to contain seven putative secondary metabolite biosynthetic gene clusters that displayed low homology (<50% coverage, <30% identity, and e-values > 0) to clusters identified within the genome of the type strain pertaining to the same species. These findings suggest that cold-adapted Arctic microbes may be a promising source of novel secondary metabolites for potential use in both industrial and medical settings.

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Streptomyces artemisiae MCCB 248 isolated from Arctic fjord sediments has unique PKS and NRPS biosynthetic genes and produces potential new anticancer natural products

Cited by 16 publications

References 30 publications

Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

Growth Promotion and Disease Suppression Ability of a Streptomyces sp. CB-75 from Banana Rhizosphere Soil

Cytotoxicity and molecular mechanism of marine-derived Streptomyces sp. GMY01 on human lung cancer cell line A549

Culture-Dependent Bioprospecting of Bacterial Isolates From the Canadian High Arctic Displaying Antibacterial Activity

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