Streptomyces apricus sp. nov., isolated from soil

Abstract

A novel Streptomyces strain, SUN51T, was isolated from soils sampled in Wisconsin, USA, as part of a Streptomyces biogeography survey. Genome sequencing revealed that this strain had less than 90 % average nucleotide identity (ANI) to type species of Streptomyces : SUN51T was most closely related to Streptomyces dioscori A217T (99.5 % 16S rRNA gene identity, 89.4 % ANI). Genome size was estimated at 8.81 Mb, and the genome DNA G+C content was 72 mol%. The strain possessed the cellular fatty acids anteiso-C15 : 0, iso-C16 : 0, 16 : 1 ω7c, anteiso-C17 : 0, iso-C14 : 0 and C16 : 0. The predominant menaquinones were MK-9 H4, MK-9 H6 and MK-9 H8. Strain SUN51T contained the polar lipids phosphatidic acid, phosphatidyl ethanolamine, phosphatidyl glycerol and diphosphatidyl glycerol. The cell wall contained ll-diaminopimelic acid. The strain could grow on a broad range of carbon sources and tolerate temperatures of up to 40 °C. The results of the polyphasic study confirmed that this isolate represents a novel species of the genus Streptomyces , for which the name Streptomyces apricus sp. nov. is proposed. The type strain of this species is SUN51T (=NRRL B-65543T=JCM 33736T).

Streptomyces beigongshangae sp. nov., isolated from baijiu fermented grains, could transform ginsenosides of Panax notoginseng

“…Through the analysis of chemotaxonomic characteristics, ll -diaminopimelic acid was measured to be the diagnostic amino acid of the cell-wall peptidoglycan, which was consistent with its similar type strain of S. apricus SUN51 T [34]. The polar lipids of REN17 T were phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid, two unidentified lipids and four unidentified glycolipids (Fig.…”

“…The predominant menaquinones of REN17 T were MK-9 (H 2 ), MK-9 (H 4 ), MK-9 (H 6 ) and MK-9 (H 8 ). Among them, MK-9 (H 2 ), MK-9 (H 4 ) and MK-9 (H 6 ) were also the predominant menaquinones of S. liliiviolaceus BH-SS-21 T and MK-9 (H 4 ), MK-9 (H 6 ) and MK-9 (H 8 ) were the predominant menaquinones of S. apricus SUN51 T [34, 35]. The major fatty acids of REN17 T were iso-C 16 : 0 (47.1 %), iso-C 16 : 1 (7.9 %) and anteiso-C 17 : 1 ω9 c (7.1 %; Table S1), among which iso-C 16 : 0 was also detected in both S. liliiviolaceus BH-SS-21 T and S. apricus SUN51 T as a major fatty acid [34, 35].…”

“…The colonies of REN17 T were irregular, dry, opaque and dense, with branched substrate mycelia and sparse aerial mycelium (ISP3 medium). In contrast to its closely related type strains, sporulation was not observed [34,35]. Physiological and biochemical characteristics of REN17 T and its closely related species in the genus of Streptomyces were compared (Table 1).…”

Microbiological exploration of the Cueva del Viento lava tube system in Tenerife, Canary Islands

“…This species plays an important role in the resistome, contributing to the synthesis of active compounds, and is known for producing antimicrobial substances, such as linocin‐M18 and curamycin (Gonzalez‐Pimentel et al, 2022 ). S. apricus , on the other hand, was isolated from soils sampled in Wisconsin (USA) (Hariharan et al, 2022 ). The presence of these Streptomyces species in Cueva del Viento highlights that they might be involved in the degradation of organic matter inside the cave.…”