Streptococcus pyogenesinfection in mice

Husmann, L K; Dillehay, Dirck L.; Jennings, Veronica M.; Scott, June R.

doi:10.1006/mpat.1996.0020

Cited by 21 publications

(31 citation statements)

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“…GAS strain B514-Sm is a spontaneous streptomycin-resistant derivative of B514/33, an M type 50 strain originally isolated from an epizootic infection of a mouse colony (22,23). UAB039 is an acapsular mutant of B514-Sm constructed by insertion of a nonreplicating plasmid within the hasA (hyaluronate synthase) gene (17).…”

Section: Methodsmentioning

confidence: 99%

CD44 as a receptor for colonization of the pharynx by group A Streptococcus

Cywes

Stamenkovic²,

Wessels³

2000

J. Clin. Invest.

123

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Section: Methodsmentioning

confidence: 99%

CD44 as a receptor for colonization of the pharynx by group A Streptococcus

Cywes

Stamenkovic²,

Wessels³

2000

J. Clin. Invest.

123

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“…B514Sm is not lethal in this model of GAS infection, although infected mice do develop necrotic lesions at the site of inoculation (data not shown). In the second model, intratracheal inoculation, B514Sm is lethal because it causes pneumonia, usually within 3 days of infection (19). The third model, intranasal inoculation, results in either long-term throat colonization or lethal pneumonia, depending on the strain of mouse used (19).…”

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confidence: 99%

“…In the second model, intratracheal inoculation, B514Sm is lethal because it causes pneumonia, usually within 3 days of infection (19). The third model, intranasal inoculation, results in either long-term throat colonization or lethal pneumonia, depending on the strain of mouse used (19). Both the intranasal and intratracheal infections mimic aspects of human GAS disease.…”

mentioning

confidence: 99%

“…Both the intranasal and intratracheal infections mimic aspects of human GAS disease. Intranasal inoculation provides information about early stages of infection, such as pharyngeal colonization, and intratracheal inoculation addresses later stages of disease, including the massive infiltration of the lung by neutrophils and macrophages, destruction of lung tissue, and spread of the GAS to cause bacteremia (19). Since GAS infections sometimes result in lethal pneumonia in humans (4,8), these models are useful for studying a specific human disease, as well as for studying the progression from pharyngitis to invasive disease and general host inflammatory responses to GAS infection (19).…”

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confidence: 99%

“…We compared the relative abilities of B514Sm and JRS584 to induce pneumonia in mice. Fifteen (B514Sm) or 21 (JRS584) female C3HeB/FeJ mice were inoculated intratracheally with 5 ϫ 10 7 to 1 ϫ 10 8 CFU of each strain as described previously (19). By 4 days postinfection, 40% of the mice inoculated with B514Sm, and 35% of the mice inoculated with JRS584, were alive (Fig.…”

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confidence: 99%

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Restoration of Mga Function to aStreptococcus pyogenesStrain (M Type 50) That Is Virulent in Mice

et al. 2001

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The Mga protein in B514Sm, a Streptococcus pyogenes strain isolated as a mouse pathogen, contains amino acid substitutions at conserved sites that render the protein defective. Replacement of mga50 with the functional homolog mga4.1 restored full expression of Mga-regulated proteins. Restoration of Mga function did not affect fibrinogen binding, nor did it affect virulence in several mouse models of group A streptococcus infection.

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Murine Models of Streptococcus pyogenes Infection

et al. 2006

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This unit describes procedures for testing virulence of Streptococcus pyogenes in mice. S. pyogenes is an important human pathogen and causes one of the most common childhood diseases. The syndromes that result from S. pyogenes infection are diverse, ranging from mild, superficial throat or skin infection to severe, invasive disea/se that is often lethal. Thus, a greater understanding of the virulence factors of this bacterium and development of modalities to prevent or relieve the infections it causes are important. Since S. pyogenes is a strictly human pathogen (with the exception of a single strain), the value of all animal models is limited. This unit describes a model for long‐term throat colonization following the natural route of infection (inhalation), one for pneumonia and systemic dissemination following intratracheal inoculation, and one for systemic dissemination following the more natural route of skin infection. In addition, methods are presented for culturing S. pyogenes from tissues of the infected animal.

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Streptococcus pyogenesinfection in mice

Cited by 21 publications

References 0 publications

CD44 as a receptor for colonization of the pharynx by group A Streptococcus

CD44 as a receptor for colonization of the pharynx by group A Streptococcus

Restoration of Mga Function to aStreptococcus pyogenesStrain (M Type 50) That Is Virulent in Mice

Murine Models of Streptococcus pyogenes Infection

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