Streptococcus porci sp. nov., isolated from swine sources

The lincosamide-resistant and macrolide-susceptible phenotype has not been described to date in Streptococcus pyogenes (group A Streptococcus; GAS). The aim of this study was to characterize a GAS isolate susceptible to macrolides but resistant to lincosamide, streptogramin A and pleuromutilin antibiotics (LS A P phenotype). The antimicrobial susceptibility was tested by the microdilution broth method and the resistance phenotype by D-test. The GAS2887HUB isolate was subjected to whole-genome sequencing. The isolate showed a positive Gots' test (clindamycin inactivation). WGS revealed the strain was ST10 and emm93 and had five resistance genes (lnu(B), ant(6)-Ia, aph(3')-III, tet(M), and dfrG). The tet(M) gene was located into a Tn916-like transposon. The lsa(E)lnu(B)-containing sequence (inserted downstream of the rumA gene) was formed by a 39.6-kb prophage, followed by a gene cluster encoding aminoglycoside-streptothricin resistance [ant(6)Iasat4aph(3')III] and lsa(E)lnu(B) genes. This structure was not transferred by conjugation. In conclusion, we have described a new genetic element carrying a determinant of lincosamide resistance in a GAS. Further 3 molecular epidemiological surveys are needed to determine the prevalence of this mechanism of resistance in GAS.

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“…The presence of a prophage highly similar to the one previously found in animal isolates of S. porci DSM 23759 [19], and E. rhusiopathiae [16,20]…”

Section: Wgs Analysis: Genetic Context Of Lsa(e)-lnu(b) Genessupporting

confidence: 62%

“…When comparing the characterized structures harbouring lsa(E)lnu (B) genes with GAS2887HUB (Fig. 1C), three findings were notable [8,16,18,19]. First, the orf4orf5lsa(E)lnu(B) structure was highly conserved (95% identity) among them.…”

Section: Wgs Analysis: Genetic Context Of Lsa(e)-lnu(b) Genesmentioning

confidence: 93%

A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics

Berbel

Càmara

Garcı́a

et al. 2019

International Journal of Antimicrobial Agents

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“…Other Streptococcus species have been isolated from clinical specimens in swine. Streptococcus plurextorum has been isolated from lesions associated with pneumonia and septicemia [ 6 ] and Streptococcus porci and Streptococcus porcorum have also been associated with pneumonia in pigs [ 7 , 8 ]. However, there are no data on the prevalence and clinical significance of these three latter species.…”

Section: Introductionmentioning

confidence: 99%

Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs

Vela

García‐Seco²,

González³

et al. 2017

PLoS ONE

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The application of MALDI-TOF MS for identifying streptococcal isolates recovered from clinical specimens of diseased pigs was evaluated. For this proposal, the MALDI BDAL Database (Bruker Daltoniks, Germany) was supplemented with the main spectrum profiles (MSP) of the reference strains of S. porci, S. porcorum and S. plurextorum associated with pneumonia and septicemia. Although these three species showed similar MALDI profiles, several peaks were recognized that can be useful for their differentiation: S. porci (4113, 6133, 7975 and 8228 m/z Da), S. plurextorum (3979, 4078, 4665, 6164, 6491, 6812, 7959 and 9330 m/z Da) and S. porcorum (3385, 3954, 4190, 6772, 7908, and 8381 m/z Da). After adding these MSPs, an evaluation was conducted to determine the accuracy of MALDI-TOF MS for the identification of streptococci from diseased pigs using 74 field isolates. Isolates were identified as S. suis, S. porcinus, S. dysgalactiae, S. hyovaginalis, S. porcorum, S. alactolyticus, S. hyointestinalis and S. orisratti. This is the first time that the latter three species have been reported from clinical specimens of pigs. Overall, there was good concordance (95.9%) between the results obtained from MALDI-TOF MS identification (best hint) and those from genotyping. Our results demonstrate the good performance of MALDI-TOF MS (100% sensitivity and specificity) for identifying most of the species of streptococci that can frequently be isolated from diseased pigs. However, conflicting results were observed in the correct identification of some isolates of S. dysgalactiae and S. alactolyticus.

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“…Public sequences with identity higher than 95% for 16S rRNA and 85% for rpoB were selected for phylogenetic study. Sequence alignments, maximum likelihood (ML) and the maximum parsimony (MP) phylogenetic trees were constructed following standard procedures (Vela et al, 2010). The reliability of the phylogenetic trees was confirmed using 1,000 bootstrap replications.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Phylogenetic Characterization of Streptococcus halichoeri from a European Badger (Meles meles) with Pyogranulomatous Pleuropneumonia

Moreno¹,

Bolea²,

Morales³

et al. 2015

Journal of Comparative Pathology

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Clinical and pathological studies in European badgers (Meles meles) are limited. Badgers play a significant role in the epidemiology of bovine tuberculosis (TB) in some countries and an accurate diagnosis is needed for this infection. However, the lesions of bovine TB are similar to those associated with other pathogens, making pathological diagnosis difficult. In the present study, Streptococcus halichoeri was isolated from a European badger with pyogranulomatous pleuropneumonia and suspected of having tuberculosis. TB and other pathogens able to induce similar lesions were ruled out. Comparative 16S rRNA and rpoB gene sequencing studies showed an identity of 99.51% and 98.28%, respectively, with S. halichoeri. This report represents the third description of this bacterium and the first in an animal species other than the grey seal (Halichoerus grypus). It also shows that S. halichoeri can be associated with a pathological process characterized by granulomatous inflammation and resembling tuberculosis.

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Streptococcus porci sp. nov., isolated from swine sources

Cited by 16 publications

References 26 publications

A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics

A novel genomic island harbouring lsa(E) and lnu(B) genes and a defective prophage in a Streptococcus pyogenes isolate resistant to lincosamide, streptogramin A and pleuromutilin antibiotics

Usefulness of MALDI-TOF MS as a Diagnostic Tool for the Identification of Streptococcus Species Recovered from Clinical Specimens of Pigs

Isolation and Phylogenetic Characterization of Streptococcus halichoeri from a European Badger (Meles meles) with Pyogranulomatous Pleuropneumonia

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