Streptococcus moroccensis sp. nov. and Streptococcus rifensis sp. nov., isolated from raw camel milk

Abstract

Two catalase- and oxidase-negative Streptococcus -like strains, LMG 27682T and LMG 27684T, were isolated from raw camel milk in Morocco. Comparative 16S rRNA gene sequencing assigned these bacteria to the genus Streptococcus with Streptococcus rupicaprae 2777-2-07T as their closest phylogenetic neighbour (95.9 % and 95.7 % similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.7 %. Although strains LMG 27682T and LMG 27684T shared a DNA–DNA hybridization value that corresponded to the threshold level for species delineation (68 %), the two strains could be distinguished by multiple biochemical tests, sequence analysis of the phenylalanyl-tRNA synthase (pheS), RNA polymerase (rpoA) and ATP synthase (atpA) genes and by their MALDI-TOF MS profiles. On the basis of these considerable phenotypic and genotypic differences, we propose to classify both strains as novel species of the genus Streptococcus , for which the names Streptococcus moroccensis sp. nov. (type strain, LMG 27682T = CCMM B831T) and Streptococcus rifensis sp. nov. (type strain, LMG 27684T = CCMM B833T) are proposed.

Streptococcus oriloxodontae sp. nov., isolated from the oral cavities of elephants

“…Huch et al, 2013;Kadri et al, 2014). In general, these studies confirm that housekeeping genes, especially when applied in a multi-locus approach, have a taxonomic resolution superior to that of 16S rRNA gene sequence analysis.…”

“…Sequence analysis of protein encoding genes is increasingly used in taxonomic studies of various lactic acid bacteria including streptococci (e.g. Huch et al, 2013;Kadri et al, 2014). In general, these studies confirm that housekeeping genes, especially when applied in a multi-locus approach, have a taxonomic resolution superior to that of 16S rRNA gene sequence analysis.…”

Streptococcus tangierensis sp. nov. and Streptococcus cameli sp. nov., two novel Streptococcus species isolated from raw camel milk in Morocco

“…Taxa 1 Streptococcus cameli CCMM B834 T ;2 Streptococcus tangierensis CCMM B832 T ; 3 Streptococcus minor LMG 21734 T (data from the present study)/CCUG 47487 T (data from (Vancanneyt et al 2004); 4 Streptococcus ovis LMG 19174 T (data from the present study)/CCUG 39485 T (data from Collins et al (2001)); 5 Streptococcus gallinaceus LMG 21849 T (data from the present study)/ CCUG 42692 T (data from Collins et al (2002)); 6 Streptococcus hyovaginalis SHV515 T (Devriese et al 1997); 7 Streptococcus henryi 126 T (Milinovich et al 2008); 8 Streptococcus sanguinis SK1 T (Kilian et al 1989); 9 Streptococcus infantis 0-122 T (Kawamura et al 1998); 10 Streptococcus rupicaprae 2777-2-07 T (Vela et al 2011); 11 Streptococcus moroccensis LMG27682 T (Kadri et al 2014); 12 Streptococcus rifensis LMG27684 T (Kadri et al 2014) ? The GenBank accession number for the 16S rRNA sequence of strain CCMM B834 T is KF999657.…”

“…Moreover, raw camel milk could be an additional source of typical dairy lactic acid bacteria (LAB) species (Janoskova and Kmet 2004). thermophilus, Streptococcus agalactiae, Streptococcus moroccensis and Streptococcus rifensis have been isolated from raw camel milk (Khedid et al 2009;Jans et al 2012;Kadri et al 2014). In recent studies, Streptococcus salivarius subsp.…”