Streptococcus agalactiae isolates of serotypes Ia, III and V from human and cow are able to infect tilapia

Chen, Ming; Wang, Rui; Luo, Fuguang; Huang, Yan; Liang, Weizheng; Huang, Ting; Lei, Aiying; Xi, Gan; Li, Liping

doi:10.1016/j.vetmic.2015.07.033

Cited by 29 publications

(26 citation statements)

References 24 publications

(40 reference statements)

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“…Experimental studies showed the transmissibility of various bovine and human GBS strains to fish [26][27][28] and characterization and genotyping studies suggested occasional transmission between humans and cattle [23,24]. Very recently, transmission of S. agalactiae through ingestion of raw fish sushi was reported to have led to severe infections in humans (Kalimuddin et al, 2017).…”

Section: Morach 2017mentioning

confidence: 99%

“…Case reports and some GBS typing data indicate possible transmission not only between human hosts and cows, but also human hosts and dogs, cats, and crocodiles [21][22][23][24][25]. In addition, experimental studies have evidenced transmission of bovine and human S. agalactiae strains to fish [26][27][28]. Still, data on interspecies transmission is scarce and strain typing studies involving a diverse set of hosts and geographical areas are missing.…”

Section: Introductionmentioning

confidence: 99%

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Population structure and virulence gene profiles of Streptococcus agalactiae collected from different hosts worldwide

Morach

Stephan

Schmitt

et al. 2017

Eur J Clin Microbiol Infect Dis

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Streptococcus agalactiae is a leading cause of morbidity and mortality among neonates and causes severe infections in pregnant women and nonpregnant predisposed adults, in addition to various animal species worldwide. Still, information on the population structure of S. agalactiae and the geographical distribution of different clones is limited. Further data are urgently needed to identify particularly successful clones and obtain insights into possible routes of transmission within one host species and across species borders. We aimed to determine the population structure and virulence gene profiles of S. agalactiae strains from a diverse set of sources and geographical origins. To this end, 373 S. agalactiae isolates obtained from humans and animals from five different continents were typed by DNA microarray profiling. A total of 242 different S. agalactiae strains were identified and further analyzed. Particularly successful clonal lineages, hybridization patterns, and strains were identified that were spread across different continents and/or were present in more than one host species. In particular, several strains were detected in both humans and cattle, and several canine strains were also detected in samples from human, bovine, and porcine hosts. The findings of our study suggest that although S. agalactiae is well adapted to various hosts including humans, cattle, dogs, rodents, and fish, interspecies transmission is possible and occurs between humans and cows, dogs, and rabbits. The virulence and resistance gene profiles presented enable new insights into interspecies transmission and make a crucial contribution to the identification of suitable targets for therapeutic agents and vaccines.

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Section: Morach 2017mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Population structure and virulence gene profiles of Streptococcus agalactiae collected from different hosts worldwide

Morach

Stephan

Schmitt

et al. 2017

Eur J Clin Microbiol Infect Dis

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“…A previous study showed that fish consumption caused a 7.3-fold increased risk for acquiring GBS serotype Ia and Ib colonization in humans ( 14 ). GBS serotypes Ia, III, and V of clonal complexes (CC) 19, CC23, and CC103 from a human and a cow have been shown to infect tilapia experimentally ( 15 ); these cross-infections support GBS pathogenicity across different species. Epidemiologic investigations and comparative genomics on a wider scale of animal and human strains may further reveal evolutionary relationships between these GBS lineages.…”

Section: The Studymentioning

confidence: 99%

Hypervirulent Clone of Group BStreptococcusSerotype III Sequence Type 283, Hong Kong, 1993–2012

Ip¹,

Ang²,

Fung³

et al. 2016

Emerg. Infect. Dis.

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“…Streptococcus agalactiae (group B streptococcus, GBS) is a leading pathogen causing sepsis and meningitis in neonates and immunocompromised patients, mastitis in dairy cattle and severe septicaemia and haemorrhaging in farmed fishes (Martinez et al ; Chen et al ; Chu et al ; Puertas‐Prieto et al ). GBS infection is restricted to the intestine and vagina in humans, to the mammary glands of cows, and to aquatic environments in which they adhere to fish.…”

Section: Introductionmentioning

confidence: 99%

“…Streptococcus agalactiae (group B streptococcus, GBS) is a leading pathogen causing sepsis and meningitis in neonates and immunocompromised patients, mastitis in dairy cattle and severe septicaemia and haemorrhaging in outbreaks of septicaemia and streptococcal toxic shock syndrome following transmission from diseased pig in China in 1998(Fong 2017), GBS is more hostspecific. However, theories have proposed that human and fish GBS may have originated from the GBS of cattle, and some paradoxical evidence even demonstrated that GBS could be transmitted among those three hosts (Martinez et al 2000;Chen et al 2015;Chu et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Investigation ofStreptococcus agalactiaeusingpcsB‐based LAMP in milk, tilapia and vaginal swabs in Haikou, China

Wang

Yang

et al. 2019

J Appl Microbiol

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Aims To develop a pcsB‐based Loop‐mediated isothermal amplification (LAMP) method for the detection of Streptococcus agalactiae (GBS) in milk, tilapia and vaginal swabs. Methods and Results The sensitivity of the LAMP method using real‐time turbidity monitoring was 1 pg of template within 1 h at 64°C, 100‐fold higher than conventional PCR. The sensitivity of visual detection dropped an order of magnitude using SYBR Green I or hydroxynaphthol blue. The validity of the visual LAMP assay was assessed by the detection of GBS in 180 vaginal swabs from one hospital, 14 brain tissues samples of diseased tilapias from two fishponds and fresh milk of 67 dairy cattle from one farm. In total, 17 samples (4 vaginal swabs, 13 tilapia brain tissues but no milk sample) tested positive for GBS. Subsequent bacterial identification confirmed the specificity and reliability of the LAMP method. Molecular serotyping and multilocus sequence typing demonstrated that all 13 tilapia GBS isolates were identical (serotype Ia, ST7), whereas the four human GBS isolates were more diverse and could be classified into two serotypes (Ia, III) and four sequence types (ST19, ST23, ST24, ST862). Virulence gene testing showed that only the bac, rib and lmb genes were not present in all isolates. Antimicrobial susceptibility profiles of the isolates were basically consistent with their genotypes, except for sulphonamide and fluoroquinolone. Conclusions We developed a reliable pcsB‐based LAMP assay for GBS detection. Our results demonstrated that the prevalence of GBS was 92·9% among diseased tilapia, 2·2% among female patients and 0% on a dairy farm in Hainan. Significance and Impact of the Study The pcsB‐based LAMP method is suitable for GBS detection and contains great potential of application in dairy industry, aquiculture and clinical.

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Streptococcus agalactiae isolates of serotypes Ia, III and V from human and cow are able to infect tilapia

Cited by 29 publications

References 24 publications

Population structure and virulence gene profiles of Streptococcus agalactiae collected from different hosts worldwide

Population structure and virulence gene profiles of Streptococcus agalactiae collected from different hosts worldwide

Hypervirulent Clone of Group BStreptococcusSerotype III Sequence Type 283, Hong Kong, 1993–2012

Investigation ofStreptococcus agalactiaeusingpcsB‐based LAMP in milk, tilapia and vaginal swabs in Haikou, China

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