Streptococcosis in cultured turbot, Scopthalmus maximus (L.), associated with Streptococcus parauberis

Domeénech, A; Derenaáandez‐Garayzábal, J F; Pascual, Cristina; García, José Ángel López; Cutuli, M. Teresa; Moreno, Miguel A.; Collins, Matthew D.; Domı́nguez, Lucas

doi:10.1111/j.1365-2761.1996.tb00117.x

Cited by 112 publications

(84 citation statements)

References 14 publications

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“…This species seems to be more frequent among British dairy herds (11). Isolation of S. parauberis from diseased fish was described by Doménech et al (9). According to the results of the present study, the species-specific regions of the described target genes of S. uberis and S. parauberis did not show any sequence variations.…”

supporting

confidence: 74%

“…In addition, 51 streptococci obtained from routine mastitis diagnostics and also S. parauberis strain 94/16 were included. The latter, kindly obtained from J. F. Fernández-Garayzábal (Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain), was originally isolated from a diseased turbot (9). The cultures isolated from routine diagnostics were preliminarily identified as S. uberis by using conventional methods (15,16).…”

mentioning

confidence: 99%

“…The latter also included the S. parauberis strain 94/16, which was originally isolated from a diseased turbot (9).…”

mentioning

confidence: 99%

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Evaluation of PCR Methods for Rapid Identification and Differentiation of Streptococcus uberis and Streptococcus parauberis

et al. 2001

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Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.The use of PCR as a diagnostic tool for the detection of bacterial pathogens has become more frequent during the past few years. The PCR technique allows the amplification of preselected, species-specific DNA regions which can be used for genotypic identification of bacteria. A molecule most suited for these purposes appears to be the gene encoding the 16S rRNA. According to Bentley et al. (4) and Bentley and Leigh (6), the sequence variability of the V2 region of the 16S rRNA allows a differentiation of 31 species of the genus Streptococcus, including the species S. uberis and S. parauberis. Both species, formerly classified as S. uberis types I and II (19), are well known as causative agents of bovine mastitis. According to biochemical and serological characteristics, the two species are almost indistinguishable (19). A molecular identification of both species can be performed by analysis of restriction fragment length polymorphisms (RFLPs) of the 16S rRNA gene (13,14,18) or by the use of species-specific oligonucleotide probes (5, 6). A major disadvantage of these procedures is the additional manipulation of the samples subsequent to the PCR and the time-consuming preparation of the gene probes. In the present study, species-specific regions of the 16S and 23S rRNA genes and the 16S-23S rRNA intergenic spacer region of S. uberis and S. parauberis were used to construct speciesspecific oligonucleotide primers. These oligonucleotide primers were used to identify and differentiate both species.A total of 17 S. uberis and 2 S. parauberis strains were used in this study, all of which were obtained from the Institute's strain collection. The cultures were identified and differentiated into both species as described previously (15, 18). In addition, 51 streptococci obtained from routine mastitis diagnostics and also S. parauberis strain 94/16 were included. The latter, kindly obtained from J. F. Fernández-Garayzábal (Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain), was originally isolated from a diseased turbot (9). The cultures isolated from routine diagnostics were preliminarily identified as S. uberis by using conventional methods (15, 16). These methods included carbohydrate fermentation tests for arabinose, fructose, inulin, lactose maltose, mannitol, raffinose, ribose, saccharose, salicin, sorbitol, and trehalose; esculin and sodium hippurate hydrolysis; determination of arginine hydrolysis; and analysis of the enzymes ␤-D-glucuronidase, pyrrolydonylaminopeptidase, and hyaluronidase. The ␤-D-glucuronidase and pyrrolydonylaminopeptidase enzyme activities were inves...

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confidence: 99%

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Evaluation of PCR Methods for Rapid Identification and Differentiation of Streptococcus uberis and Streptococcus parauberis

et al. 2001

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“…The streptococcal infections usually cause high mortality and last a long period of time (Yanong and Francis-Floyd, 2006). To date, streptococcal pathogens of fish comprise Streptococcus iniae, Streptococcus milleri (Yanong and Francis-Floyd, 2006), Streptococcus parauberis (Doménech et al, 1996), Streptococcus agalactiae (Evans et al, 2002), Streptococcus ictaluri (Shewmaker et al, 2007), and Streptococcus dysgalactiae (Nomoto et al, 2004;Vossen et al, 2004;Zhou et al, 2007). Among these species, S. dysgalactiae has been reported as fish pathogen with low frequency while S. iniae reported with high frequency.…”

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confidence: 99%

Isolation and characterization of Streptococcus dysgalactiae from diseased Acipenser schrenckii

Yang

2009

Aquaculture

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“…canis is also very much greater than that which may be expected between different strains of the same species. For example, in the case of streptococcal species, levels of sequence divergence of 0n5 % or less, have been reported for partial and\or near complete 16S rRNAs of strains of the same species [Streptococcus pluranimalium (Devriese et al, 1999) ; Streptococcus parasanguinis (Fernandez Garayzabal et al, 1998) ; S. parauberis (Domenech et al, 1996) ; Streptococcus hyovaginalis and Streptococcus thoraltensis (Devriese et al, 1997) ; Streptococcus infantis and Streptococcus peroris (Kawamura et al, 1998)]. This is also the case for all other Gram-positive, catalasenegative, coccus-shaped taxa for which intra-specific sequence divergence values are available (e.g.…”

Section: Streptococcus Agalactiae S Canis Streptococcus Dysgalactimentioning

confidence: 99%

An unusual Streptococcus from human urine, Streptococcus urinalis sp. nov.

Collins¹,

Hutson²,

Falsen³

et al. 2000

International Journal of Systematic and Evolutionary Microbiology

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Biochemical, molecular chemical and molecular genetic studies were performed on an unknown Gram-positive, catalase-negative, chain-forming coccus isolated from the urine of a patient suffering from cystitis. Comparative 16S rRNA gene sequencing showed that the organism is a member of the ' pyogenic subgroup ' of the genus Streptococcus and has a close affinity with Streptococcus pyogenes and Streptococcus canis. The unknown coccus was, however, readily distinguished from these species and other streptococci by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phenotypic and phylogenetic evidence, it is proposed that the unknown bacterium be classified as a new species of the genus Streptococcus, Streptococcus urinalis sp. nov. The type strain of Streptococcus urinalis is CCUG 41590 T .

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Streptococcosis in cultured turbot, Scopthalmus maximus (L.), associated with Streptococcus parauberis

Cited by 112 publications

References 14 publications

Evaluation of PCR Methods for Rapid Identification and Differentiation of Streptococcus uberis and Streptococcus parauberis

Evaluation of PCR Methods for Rapid Identification and Differentiation of Streptococcus uberis and Streptococcus parauberis

Isolation and characterization of Streptococcus dysgalactiae from diseased Acipenser schrenckii

An unusual Streptococcus from human urine, Streptococcus urinalis sp. nov.

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