Streptococcosis

Gottschalk, Marcelo; Segura, Mariela

doi:10.1002/9781119350927.ch61

Cited by 83 publications

(142 citation statements)

References 142 publications

Supporting

Mentioning

116

Contrasting

Unclassified

Order By: Relevance

“…A high genetic and phenotypic diversity of S. suis strains within serotype 2 is reported according to geographical distribution [4]. Pigs are affected generally between 5 and 10 weeks of age, when passive immunity provided by colostrum decreases [5,6]. The pathogenesis of S. suis infection is not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants

et al. 2017

Self Cite

View full text Add to dashboard Cite

An in vitro porcine bone marrow-derived dendritic cell (DC) culture was developed as a model for evaluating immune polarization induced by adjuvants when administered with immunogens that may become vaccine candidates if appropriately formulated. The swine pathogen Streptococcus suis was chosen as a prototype to evaluate proposed S. suis vaccine candidates in combination with the adjuvants Poly I:C, Quil A ®, Alhydrogel ®, TiterMax Gold ® and Stimune ®. The toll-like receptor ligand Poly I:C and the saponin Quil A ® polarized swine DC cytokines towards a type 1 phenotype, with preferential production of IL-12 and TNF-α. The water-in-oil adjuvants TiterMax Gold ® and Stimune ® favoured a type 2 profile as suggested by a marked IL-6 release. In contrast, Alhydrogel ® induced a type 1/type 2 mixed cytokine profile. The antigen type differently modified the magnitude of the adjuvant effect, but overall polarization was preserved. This is the first comparative report on swine DC immune activation by different adjuvants. Although further swine immunization studies would be required to better characterize the induced responses, the herein proposed in vitro model is a promising approach that helps assessing behaviour of the vaccine formulation rapidly at the pre-screening stage and will certainly reduce numbers of animals used while advancing vaccinology science.

show abstract

Section: Introductionmentioning

confidence: 99%

Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Streptococcus suis ( S. suis ) is a pathogen for both pigs and humans, that is endemically present in commercial pig herds worldwide [ 1 ], and seems to be emerging in humans [ 2 ]. The natural habitat of S. suis in pigs is the upper respiratory tract (especially the tonsils and nasal cavities), as well as the gastrointestinal and genital tracts [ 1 , 3 , 4 , 5 ]. The respiratory route is generally thought to be the main route of horizontal transmission among pigs, and especially the tonsils are considered important reservoirs of S. suis [ 1 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…The natural habitat of S. suis in pigs is the upper respiratory tract (especially the tonsils and nasal cavities), as well as the gastrointestinal and genital tracts [ 1 , 3 , 4 , 5 ]. The respiratory route is generally thought to be the main route of horizontal transmission among pigs, and especially the tonsils are considered important reservoirs of S. suis [ 1 , 5 ]. According to the most accepted hypothesis, S. suis breaches the mucosal epithelium at either the upper respiratory tract or the gastrointestinal tract, and reaches the bloodstream, leading to systemic infection [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Simultaneous Exposure of Pigs to Streptococcus suis Serotypes 2 and 9 on Their Colonization and Transmission, and on Mortality

et al. 2017

View full text Add to dashboard Cite

The distribution of Streptococcus suis serotypes isolated from clinically infected pigs differs between geographical areas, and varies over time. In several European countries, predomination of serotype 2 has changed to serotype 9. We hypothesize a relation, with one serotype affecting the other in colonization and invasion. The aim of this study was to evaluate whether simultaneous exposure of pigs to serotypes 2 and 9 affects colonization and transmission of each type, and mortality. Thirty-six caesarean-derived/colostrum-deprived piglets were randomly assigned to three groups, and there housed pair-wise. At six weeks old, one pig per pair was inoculated with either one (serotype 2 or 9; mono-group) or two serotypes simultaneously (dual-group); the other pig was contact-exposed. Tonsillar and nasal samples were collected within three weeks post inoculation. Bacterial loads in samples were quantified using multiplex real-time polymerase chain reaction (PCR). Transmission rates of the serotypes among pigs were estimated using a mathematical Susceptible-Infectious (SI) model. Bacterial loads and transmission rates did not differ significantly between serotypes. Compared to the mono-group, in the dual-group the average serotype 2 load in tonsillar samples from contact pigs was reduced on days 1 to 4 and on day 6. Simultaneous exposure to the serotypes reduced the mortality hazard 6.3 times (95% C.I.: 2.0–19.8) compared to exposure to serotype 2 only, and increased it 6.6 times (95% C.I.: 1.4–30.9) compared to exposure to serotype 9 only. This study indicates that serotype 2 load and mortality were affected in pigs exposed to these two serotypes.

show abstract

“…S. suis disease is a zoonotic infectious disease caused by S. suis, which can induce the death in humans, and is described as a pathogen for people in contact with swine or raw pork products [1]. S. suis, a Gram-positive encapsulated coccus, is a causative agent of serious zoonotic diseases with clinical manifestations of meningitis, septicemia, arthritis, pneumonia, endocarditis, and even acute death in pigs and humans [2].…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity of a Strain of Streptococcus suis Type 2 to Mice and Swine Isolated from Jingzhou City

Liu

Yang

et al. 2019

BJI

View full text Add to dashboard Cite

Aims: The objective of this study is to identify S. suis type 2 and evaluate the virulence of ZHJ01 strain isolation, and verity the clinical and pathological outcome of a systemic infection caused by one serotype 2 when simultaneously inoculated with ZHJ01 strain. We also want to clarify the epidemiologic, clinical, microbiologic characteristics and the pathogenesis mechanism of S. suis type 2 in Hubei province, China. Study Design: Pigs suspected of being infected with S. suis in Jingzhou regions of Hubei province, China were studied. S. suis type 2 isolation was obtained from the suspicion of diseased pig. The case of S. suis type 2 was detected by the virulence factor amplification based on PCR detection and bacterial isolation, identification in the laboratory. According to the experimental infections of mice and piglets, pathogenicity of this S. suis type 2 isolation to mice and swine was monitored. This study was conducted in the key laboratory of pathogenic microbiology, College of Animal Science of Yangtze University, and Institute of Black Pigs Research, Yangtze University. Methodology: Proper serological typing can be performed using a co-agglutination test. The typical colonies purificated and cultured were inoculated with Glucose, Lactose, Raffinos, Sorbitol, D (+)-sucrose, Trehalose, 6.5%NaCl, D (-)-Salicin, Hippurate, Esculin hydrate, V-p, etc., then the test results were recorded. Detection of virulence factors were performed using PCR amplification and DNA sequencing. S.suis type 2 isolation was inoculated to mice and piglets for the virulence test, and the observation of the clinical signs and pathological changes. Results: The virulence factor of extracellular protein factor (EF) was determined from ZHJ01 strain based on PCR detection. Sequence analysis indicated that the isolate was very similar to nucleotide homology with others SS2 strains from different county or contries, and there was not much variation. LD50 of S. suis type 2 for mice was 2.5 x 107cfu. LD50 of S. suis type 2 for piglets was 3.92 x 109cfu. Conclusion: The results show that Swine S. suis type 2 has a relatively strong pathogenicity to pigs in Hubei province, China. This study can be, in part, sufficient to explain the pathogenicity for ZHJ01 strain in area of Zhijing, Jingzhou city, China, which may provide insights into the pathogenesis SS2 and more valid data to support the development of S. suis vaccine as well as the epidemiological investigation, further monitoring and effective prevention to S. suis.

show abstract

Streptococcosis

Cited by 83 publications

References 142 publications

Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants

Porcine Dendritic Cells as an In Vitro Model to Assess the Immunological Behaviour of Streptococcus suis Subunit Vaccine Formulations and the Polarizing Effect of Adjuvants

Effect of Simultaneous Exposure of Pigs to Streptococcus suis Serotypes 2 and 9 on Their Colonization and Transmission, and on Mortality

Pathogenicity of a Strain of Streptococcus suis Type 2 to Mice and Swine Isolated from Jingzhou City

Contact Info

Product

Resources

About