StreptoBase: An Oral Streptococcus mitis Group Genomic Resource and Analysis Platform

Zheng, Wenning; Tan, Tze King; Paterson, Ian C.; Mutha, Naresh V.R.; Siow, Cheuk Chuen; Tan, Shi; Old, Lesley A.; Jakubovics, Nicholas S.; Choo, Siew Woh

doi:10.1371/journal.pone.0151908

Cited by 30 publications

(34 citation statements)

References 41 publications

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“…Mitis group streptococci are genetically similar species and include S. mitis , S. pneumoniae , S. australis , S. peroris , S. cristatus , S. gordonii , S. infantis , S. oligofermentans , S. oralis , S. parasanguinis , S. pseudopneumoniae , S. tigurinus , and S. sanguinis [ 13 ]. These species are colonized into oral cavity and stimulate mucosal immunity permanently.…”

Section: Discussionmentioning

confidence: 99%

Autolysin (lytA) recombinant protein: a potential target for developing vaccines against pneumococcal infections

Afshar

Rafiee

Kheirandish

et al. 2020

Clin Exp Vaccine Res

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Purpose N-acetylmuramoyl-l-alanine amidase known as lytA, is an immunogenic protein that plays an important role in the pathogenesis of Streptococcus pneumoniae . It is highly conserved among S. pneumoniae strains and is absent among other Streptococcus species. In the present study, the level of antibodies against the lytA recombinant protein was evaluated in healthy individuals' sera. Materials and Methods DNA was extracted from S. pneumoniae ATCC 49619 to amplify lyt A gene by polymerase chain reaction assay. The lytA amplicon and p ET28a vector were separately double digested using Nde -1 and Xho 1 restriction enzymes and then ligated together with ligase enzyme. The recombinant plasmid was expressed in Escherichia coli BL21 strain and the lytA recombinant protein purified using nickel-nitrilotriacetic acid affinity chromatography. Western blot was carried to detect lytA recombinant protein. Sixty healthy individual's sera (at three age groups: group 1, <2; group 2, 2–40; and group 3, 60–90 years old) were collected and the titers of anti-lytA antibodies were determined. Results The lyt A gene was highly expressed in E. coli BL21 host. The recombinant lytA protein was purified and confirmed by western blotting. Tukey test analysis showed that there were no significant differences among the age groups considering the anti-lytA titer of 10. However, at the anti-lytA titer of 60, significant differences were observed between group 1 vs. group 2 (p<0.001); group 1 vs. group 3 (p=0.003), and group 2 vs. group 3 (p=0.024). Conclusion The lytA protein seems to be a highly immunogenic antigen and a potential target for developing vaccines against pneumococcal infections.

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Section: Discussionmentioning

confidence: 99%

Autolysin (lytA) recombinant protein: a potential target for developing vaccines against pneumococcal infections

Afshar

Rafiee

Kheirandish

et al. 2020

Clin Exp Vaccine Res

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“…gordonii (SK12) was isolated from the oral cavity of a volunteer in Denmark by Kilian and colleagues (6). The genomic DNA was extracted as previously described (7). The SK12 strain was sequenced using Illumina HiSeq 2000 platform (8).…”

Section: Methodsmentioning

confidence: 99%

Genome Analysis of Streptococcus gordonii SK12

et al. 2014

ADUM

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“…In general, these micro-organisms show low pathogenicity. Nevertheless, in some cases, they can enter the bloodstream, potentially causing infective endocarditis, brain abscesses or abdominal infections, as well as other diseases [3].…”

Section: Full-textmentioning

confidence: 99%

Streptococcus downii sp. nov., isolated from the oral cavity of a teenager with Down syndrome

Martínez-Lamas

Limeres

Dios

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A new α-haemolytic streptococcal strain has been isolated from the dental plaque of a teenager with Down syndrome. Genetic and taxonomic analyses place this Streptococcus within the oralis group. It is a Gram-stain-positive, non-motile, non-spore-forming spherical alpha-haemolytic coccus arranged in chains, and it ferments a large number of monosaccharides and disaccharides, as well as polymeric carbohydrates. It differs biochemically from closely related species of Streptococcus due to its production of α-galactosidase, β-galactosidase and N-acetyl-β-d-glucosaminidase and by the absence of arginine dihydrolase deiminase and IgA1-protease. It grows in a temperature range of 25 to 40 °C (optimal growth temperature at 37 °C) and in a pH range of 4.5 to 8 (optimal pH at 7.0). A phylogenetic analysis based on its 16S and 23S rRNA gene sequences placed it close to Streptococcus dentisani CECT 7747T. The ANIb and ANIm values were 93.19 and 93.61 %, respectively, both below the accepted threshold to designate it as a new species of bacteria. A phylogenetic tree based on its core genome placed it close to Streptococcus oralis subsp. dentisani strain CECT 7747T with a distance in the expanded core phylogeny of 0.1298. The in silico DNA–DNA hybridization value was 52.2 % with respect to the closest species, S. oralis subsp. dentisani CECT 7747T. Based on these data, a new species of bacteria within the genus Streptococcus , family Streptococcaceae and order Lactobacillales is described, for which the name of Streptococcus downii sp. nov. is proposed (type strain CECT 9732T=CCUG 73139T).

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StreptoBase: An Oral Streptococcus mitis Group Genomic Resource and Analysis Platform

Cited by 30 publications

References 41 publications

Autolysin (lytA) recombinant protein: a potential target for developing vaccines against pneumococcal infections

Autolysin (lytA) recombinant protein: a potential target for developing vaccines against pneumococcal infections

Genome Analysis of Streptococcus gordonii SK12

Streptococcus downii sp. nov., isolated from the oral cavity of a teenager with Down syndrome

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