Streptavidin-exposed magnetic nanoparticles for lectin magnetic separation (LMS) of Staphylococcus aureus prior to three quantification strategies

Yang, Guotai; Huang, Min; Wang, Yutong; Chen, Guanhua; Zhao, Yu; Xu, Hengyi

doi:10.1007/s00604-019-3978-4

Cited by 8 publications

(4 citation statements)

References 22 publications

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“…During cell separation, MNPs are bound to specific biorecognition molecules such as antigens, glycoproteins, and specific structural components on the cell surface by Magnetic Activated Cell Sorting (MACS) [ 110 , 111 , 112 ], nucleic acid probes, antibiotics, phages [ 113 ], and lectins [ 114 , 115 ]. Subsequently, the MNP-labeled cells are separated from the mixtures through the application of a magnetic field, leading to the enrichment and purification of the target cells.…”

Section: Applications Of Mnps In Biomedical Fieldsmentioning

confidence: 99%

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

Hong,

Wang,

Zheng

et al. 2024

Materials

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Magnetic nanoparticles (MNPs) have found extensive application in the biomedical domain due to their enhanced biocompatibility, minimal toxicity, and strong magnetic responsiveness. MNPs exhibit great potential as nanomaterials in various biomedical applications, including disease detection and cancer therapy. Typically, MNPs consist of a magnetic core surrounded by surface modification coatings, such as inorganic materials, organic molecules, and polymers, forming a nucleoshell structure that mitigates nanoparticle agglomeration and enhances targeting capabilities. Consequently, MNPs exhibit magnetic responsiveness in vivo for transportation and therapeutic effects, such as enhancing medical imaging resolution and localized heating at the site of injury. MNPs are utilized for specimen purification through targeted binding and magnetic separation in vitro, thereby optimizing efficiency and expediting the process. This review delves into the distinctive functional characteristics of MNPs as well as the diverse bioactive molecules employed in their surface coatings and their corresponding functionalities. Additionally, the advancement of MNPs in various applications is outlined. Additionally, we discuss the advancements of magnetic nanoparticles in medical imaging, disease treatment, and in vitro assays, and we anticipate the future development prospects and obstacles in this field. The objective is to furnish readers with a thorough comprehension of the recent practical utilization of MNPs in biomedical disciplines.

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Section: Applications Of Mnps In Biomedical Fieldsmentioning

confidence: 99%

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

Hong,

Wang,

Zheng

et al. 2024

Materials

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“…Hyun et al (2020) devised a method for selectively eliminating harmful bacteria by using bacterial lectin-targeting glycoconjugates containing an epitope or a photosensitizer to enhance antibody-dependent cellular cytotoxicity (ADCC) or photodynamic treatment (PDT). Furthermore, Yang et al (2019a) employed wheat germ agglutinin lectin (WGA) as a bio-recognition agent for S. aureus enrichment and found that even at 100-1000 CFU/ml of S. aureus, WGA demonstrated high enrichment efficiency. Later, this unique enrichment was applied in a quantitative and selective detection technique to detect S. aureus with a detection limit of 3.5 102 CFU/ml using double stranded DNA (dsDNA) stabilized gold nanoparticles (AuNPs).…”

Section: Applications Of Lectins In Food Safety Pathogen Separationmentioning

confidence: 99%

Monitoring of Microbial Safety of Foods Using Lectins: A Review

Vishweswaraiah

Tenguria

Chandrasekhar

et al. 2022

Front. Food. Sci. Technol.

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Food-borne diseases are on the rise, and these will likely continue as a public health concern into the coming decades. Majority of foodborne outbreaks are linked to infections by emerging foodborne pathogens such as Campylobacter, Salmonella, Listeria monocytogenes, and Escherichia coli O157:H7. Foodborne pathogen identification becomes crucial in such scenarios to control these pathogens, associated outbreaks, and diseases. Pathogen detection systems have evolved as essential food safety tools to combat microbial threats and experts are striving to develop robust, accurate and ergonomic rapid pathogen-detection kits. Lectin, a ubiquitous biomolecule (sugar binding proteins) present in almost all domains of life is a promising alternative to molecular based methods as a bio-recognition molecule in detection of foodborne pathogens for biosensor applications, owing to its multivalency and spatial organization of ligands. Due to their extensive prevalence, lectin-based biosensors have become the most sought-after bio-recognition molecules in biosensor applications because of increased sensitivity and reduced cost when compared to immune-based biosensors. The current paper discusses the claimed benefits of lectin as a superior bio-recognition molecule, as well as its numerous applications in biosensor creation.

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“…However, the expensive, unstable, and difficult-to-store properties of antibodies limit their application in underdeveloped regions. Therefore, a number of low-cost, stable antibody alternatives have been developed for simultaneous recognition and separation of multiple bacteria, such as antibiotics [20], lectins [21], aptamer [22], and polymers [23]. Studies have shown that the bacterial surface is rich in lipopolysaccharide, teichoic acid, and other components, which make the bacterial surface negatively charged [24].…”

Section: Introductionmentioning

confidence: 99%

Rapid-Response Magnetic Enrichment Strategy for Significantly Improving Sensitivity of Multiplex PCR Analysis of Pathogenic Listeria Species

et al. 2022

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Listeria monocytogenes and Listeria ivanovii are important pathogenic Listeria spp. that cause infections in humans and animals. Establishing a rapid and sensitive method for the simultaneous screening of pathogenic Listeria spp. is of great significance for ensuring food safety. Multiplex polymerase chain reaction (mPCR) has been extensively reported to simultaneously detect several pathogens in food with high sensitivity, but a time-consuming pre-enrichment process is necessary. In this study, we report the usage of surface-modified polyethyleneimine-coated positively charged magnetic nanoparticles (PEI-MNPs) for rapid enrichment of pathogenic Listeria spp. through electrostatic interactions. The enrichment process takes only 10 min with high capture efficiency (more than 70%) at a wide pH range and ionic strength. Combined with mPCR analysis, the PEI-MNPs-mPCR strategy can simultaneously, rapidly, and sensitively detect pathogenic Listeria spp. without a time-consuming pre-concentration process. Under the optimal conditions, the detection limits of L. monocytogenes and L. ivanovii in lettuce were both as low as 101 CFU/mL, which was a hundred times lower than that without magnetic enrichment. In conclusion, the magnetic enrichment strategy based on charge interaction combined with mPCR analysis has great application potential in shortening the pre-concentration time of foodborne pathogens and improving the detection sensitivity.

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Streptavidin-exposed magnetic nanoparticles for lectin magnetic separation (LMS) of Staphylococcus aureus prior to three quantification strategies

Cited by 8 publications

References 22 publications

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

The Recent Applications of Magnetic Nanoparticles in Biomedical Fields

Monitoring of Microbial Safety of Foods Using Lectins: A Review

Rapid-Response Magnetic Enrichment Strategy for Significantly Improving Sensitivity of Multiplex PCR Analysis of Pathogenic Listeria Species

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