Streptavidin-biotin system-mediated immobilization of a bivalent nanobody onto magnetosomes for separation and analysis of 3-phenoxybenzoic acid in urine

Tang, Fang; Wang, Yating; Wang, Di; Yang, Yayun; Chang, Jiashu; Sun, Huabo; Gu, Shaopeng; He, Jinxin

doi:10.1039/d4ay00026a

Anal. Methods

2024

DOI: 10.1039/d4ay00026a

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Streptavidin-biotin system-mediated immobilization of a bivalent nanobody onto magnetosomes for separation and analysis of 3-phenoxybenzoic acid in urine

Fang Tang,

Yating Wang,

Di Wang

et al.

Abstract: The compound 3-phenoxybenzoic acid (3-PBA) is frequently utilized as a biomarker to detect exposure to various pyrethroids. In this study, bivalent nanobody (Nb2) specifically targeting 3-PBA were biotinylated and immobilized...

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Cited by 3 publications

References 30 publications

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Enhancing the sensitivity of immunomagnetic assay for 3-phenoxybenzonic acid: a novel approach utilizing magnetosome-nanobody complexes and gold nanoparticles probes

He,

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Zhao

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Enhancing the sensitivity of immunomagnetic assay for 3-phenoxybenzonic acid: a novel approach utilizing magnetosome-nanobody complexes and gold nanoparticles probes

He,

Wang,

Zhao

et al. 2024

Microchim Acta

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Determination of 3-Phenoxybenzoic Acid by an Improved Enzyme-Linked Immunosorbent Assay (ELISA) by the Immobilization of Nanobodies with Streptavidin and Biotin

Tang,

Chen,

Zhao

et al. 2024

Analytical Letters

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Biominerals and Bioinspired Materials in Biosensing: Recent Advancements and Applications

Abdelhamid,

Ki,

Pack

2024

IJMS

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Inspired by nature’s remarkable ability to form intricate minerals, researchers have unlocked transformative strategies for creating next-generation biosensors with exceptional sensitivity, selectivity, and biocompatibility. By mimicking how organisms orchestrate mineral growth, biomimetic and bioinspired materials are significantly impacting biosensor design. Engineered bioinspired materials offer distinct advantages over their natural counterparts, boasting superior tunability, precise controllability, and the ability to integrate specific functionalities for enhanced sensing capabilities. This remarkable versatility enables the construction of various biosensing platforms, including optical sensors, electrochemical sensors, magnetic biosensors, and nucleic acid detection platforms, for diverse applications. Additionally, bioinspired materials facilitate the development of smartphone-assisted biosensing platforms, offering user-friendly and portable diagnostic tools for point-of-care applications. This review comprehensively explores the utilization of naturally occurring and engineered biominerals and materials for diverse biosensing applications. We highlight the fabrication and design strategies that tailor their functionalities to address specific biosensing needs. This in-depth exploration underscores the transformative potential of biominerals and materials in revolutionizing biosensing, paving the way for advancements in healthcare, environmental monitoring, and other critical fields.

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Streptavidin-biotin system-mediated immobilization of a bivalent nanobody onto magnetosomes for separation and analysis of 3-phenoxybenzoic acid in urine

Abstract: The compound 3-phenoxybenzoic acid (3-PBA) is frequently utilized as a biomarker to detect exposure to various pyrethroids. In this study, bivalent nanobody (Nb2) specifically targeting 3-PBA were biotinylated and immobilized...

Cited by 3 publications

References 30 publications

Enhancing the sensitivity of immunomagnetic assay for 3-phenoxybenzonic acid: a novel approach utilizing magnetosome-nanobody complexes and gold nanoparticles probes

Enhancing the sensitivity of immunomagnetic assay for 3-phenoxybenzonic acid: a novel approach utilizing magnetosome-nanobody complexes and gold nanoparticles probes

Determination of 3-Phenoxybenzoic Acid by an Improved Enzyme-Linked Immunosorbent Assay (ELISA) by the Immobilization of Nanobodies with Streptavidin and Biotin

Biominerals and Bioinspired Materials in Biosensing: Recent Advancements and Applications

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