Strenuous exercise and quercetin intake stimulates aortic NFkB signaling

Abstract

NF‐kB belongs to a family of structurally related inducible transcription factors known to be activated in the vessel wall in atherosclerosis and other diseases NF‐kB activation is mediated by diverse extracellular signals including oxidized LDL, and inflammatory cytokines. In the current study we combined quercetin and exercise to examine the effect of the combination.Study Design and MethodsWe investigated the combination of exercise and quercetin intake in C57BL6 normal male mice fed normal mouse chow. Mice were divided into four groups. These groups are as follows: Control mice, left untreated; control quercetin group, orally supplied with 100 μg/day of quercetin without exercising; exercise group without quercetin, and exercise group with quercetin supplements. The exercise groups were run on a treadmill for 30 minutes, 30m/m/ 5 days/week for 60 days. At the end of the study, mice were sacrificed tissues such as the aorta, adipose and livers were collected for gene expressions for genes associated with NFkB signaling, inflammation, and lipoprotein metabolism were analyze.Results and DiscussionNFkB mRNA levels was significantly (P<0.05) upregulated with the combination of exercise and quercetin intake, however on the other hand exercise or quercetin intake alone have resulted in significant (P<0.05) down regulation of its mRNA levels.ConclusionThis study demonstrated that quercetin intake with exercise modulate NFkB signaling and its associated genes.